scholarly journals Functional regeneration of tissue engineered skeletal muscle in vitro is dependent on the inclusion of basement membrane proteins

Cytoskeleton ◽  
2019 ◽  
Vol 76 (6) ◽  
pp. 371-382 ◽  
Author(s):  
Jacob W. Fleming ◽  
Andrew J. Capel ◽  
Rowan P. Rimington ◽  
Darren J. Player ◽  
Alexandra Stolzing ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Membrane Proteins ◽  
Basement Membrane ◽  
Basement Membrane Proteins ◽  
Functional Regeneration

Cancer progression is associated with increased expression of basement membrane proteins in three-dimensional in vitro models of human oral cancer

2009 ◽  
Vol 54 (10) ◽  
pp. 924-931 ◽  
Author(s):  
Keerthi K. Kulasekara ◽  
Ochiba M. Lukandu ◽  
Evelyn Neppelberg ◽  
Olav K. Vintermyr ◽  
Anne Christine Johannessen ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Membrane Proteins ◽  
Basement Membrane ◽  
Cancer Progression ◽  
Three Dimensional ◽  
In Vitro Models ◽  
Basement Membrane Proteins

Matrix metalloproteinase activity is required for activity-induced angiogenesis in rat skeletal muscle

2000 ◽  
Vol 279 (4) ◽  
pp. H1540-H1547 ◽  
Author(s):  
T. L. Haas ◽  
M. Milkiewicz ◽  
S. J. Davis ◽  
A. L. Zhou ◽  
S. Egginton ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Membrane Proteins ◽  
Basement Membrane ◽  
Critical Role ◽  
Rat Skeletal Muscle ◽  
Protein Levels ◽  
Capillary Basement Membrane ◽  
Basement Membrane Proteins ◽  
Muscle Inhibition ◽  
Membrane Type

Proteolysis of the capillary basement membrane is a hallmark of inflammation-mediated angiogenesis, but it is undetermined whether proteolysis plays a critical role in the process of activity-induced angiogenesis. Matrix metalloproteinases (MMPs) constitute the major class of proteases responsible for degradation of basement membrane proteins. We observed significant elevations of mRNA and protein levels of both MMP-2 and membrane type 1 (MT1)-MMP (2.9 ± 0.7- and 1.5 ± 0.1-fold above control, respectively) after 3 days of chronic electrical stimulation of rat skeletal muscle. Inhibition of MMP activity via the inhibitor GM-6001 prevented the growth of new capillaries as assessed by the capillary-to-fiber ratio (1.34 ± 0.08 in GM-6001-treated muscles compared with 1.69 ± 0.03 in control 7-day-stimulated muscles). This inhibition correlated with a significant reduction in the number of capillaries with observable breaks in the basement membrane, as assessed by electron microscopy (0.27 ± 0.27% in GM-6001-treated muscles compared with 3.72 ± 0.65% in control stimulated muscles). Proliferation of capillary-associated cells was significantly elevated by 2 days and remained elevated throughout 14 days of stimulation. Capillary-associated cell proliferation during muscle stimulation was not affected by MMP inhibition (80.3 ± 9.3 nuclei in control and 63.5 ± 8.5 nuclei in GM-6001-treated animals). We conclude that MMP proteolysis of capillary basement membrane proteins is a critical component of physiological angiogenesis, and we postulate that capillary-associated proliferation precedes and occurs independently of endothelial cell sprout formation.

scholarly journals Intestinal cells produce basement membrane proteins in vitro.

Gut ◽  
1987 ◽  
Vol 28 (Suppl) ◽  
pp. 143-151 ◽  
Author(s):  
U Hahn ◽  
D Schuppan ◽  
E G Hahn ◽  
H J Merker ◽  
E O Riecken
Keyword(s):  
Membrane Proteins ◽  
Basement Membrane ◽  
Intestinal Cells ◽  
Basement Membrane Proteins
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