Strong cytoskeleton activity on millisecond timescales upon particle binding revealed by ROCS microscopy

Cytoskeleton ◽  
2018 ◽  
Vol 75 (9) ◽  
pp. 410-424 ◽  
Author(s):  
Felix Jünger ◽  
Alexander Rohrbach
Keyword(s):  
Particle Binding

An electrochemical sensor for selective TNT sensing based on Tobacco mosaic virus-like particle binding agents

2014 ◽  
Vol 50 (85) ◽  
pp. 12977-12980 ◽  
Author(s):  
Faheng Zang ◽  
Konstantinos Gerasopoulos ◽  
Xiao Zhu Fan ◽  
Adam D. Brown ◽  
James N. Culver ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Electrochemical Sensor ◽  
Mosaic Virus ◽  
Virus Like Particle ◽  
Binding Agents ◽  
Particle Binding

scholarly journals Influence of dsDNA fragment length on particle binding in an evanescent field biosensing system

The Analyst ◽  
2014 ◽  
Vol 139 (7) ◽  
pp. 1672-1677 ◽  
Author(s):  
Marjo Koets ◽  
Kim van Ommering ◽  
Liqin Wang ◽  
Emilie Testori ◽  
Toon H. Evers ◽  
...  
Keyword(s):  
Fragment Length ◽  
Evanescent Field ◽  
Particle Binding ◽  
Biosensing System

scholarly journals Macrophage and Retinal Pigment Epithelium Phagocytosis

1999 ◽  
Vol 190 (6) ◽  
pp. 861-874 ◽  
Author(s):  
Silvia C. Finnemann ◽  
Enrique Rodriguez-Boulan
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Insoluble Fraction ◽  
Αvβ3 Integrin ◽  
Apoptotic Cells ◽  
Integrin Receptor ◽  
Photoreceptor Outer Segment ◽  
Particle Binding ◽  
Pkc Activation

Noninflammatory monocyte macrophages use αvβ3 integrin to selectively bind apoptotic cells, initiating their phagocytic removal. In a related process, the retinal pigment epithelium (RPE) employs αvβ5 integrin to recognize spent photoreceptor outer segment particles (OS). Here, we show that apoptotic cells and OS compete for binding to these receptors, indicating that OS and apoptotic cells expose surface signals recognizable by αvβ3 and αvβ5. Particle binding to αvβ5 required protein kinase C (PKC) activation. In RPE, αvβ5 binding was maximally activated even before any phagocytic challenge and was reduced by PKC inhibitors. In macrophages, it was dormant but became activated upon PKC stimulation. PKC-activated αvβ5-mediated binding in macrophages differed from constitutive binding to the same integrin receptor in RPE cells in that the former followed much faster kinetics, similar to particle binding mediated by αvβ3. Activation of αvβ5 for particle binding correlated with its recruitment into a detergent-insoluble fraction, a process sensitive to pharmacological modulation of PKC in both types of phagocytes. Furthermore, αvβ5 but not αvβ3 particle binding required actin microfilaments. These data constitute the first evidence that noninflammatory phagocytes actively regulate the earliest phase of phagocytic clearance, particle binding, by controlling receptor activity.

scholarly journals Vascular-targeted particle binding efficacy in the presence of rigid red blood cells: Implications for performance in diseased blood

2018 ◽  
Vol 12 (4) ◽  
pp. 042217 ◽  
Author(s):  
Mario Gutierrez ◽  
Lauro Sebastian Ojeda ◽  
Omolola Eniola-Adefeso
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Particle Binding
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