Actin assembly mediated by a nucleation promoting factor WASH is involved in MTOC–TMA formation during Xenopus oocyte maturation

Cytoskeleton ◽  
2018 ◽  
Vol 75 (3) ◽  
pp. 131-143 ◽  
Author(s):  
Yuka Yamagishi ◽  
Hiroshi Abe
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte ◽  
Actin Assembly ◽  
Nucleation Promoting Factor

c-Mos and cyclin B/cdc2 connections during Xenopus oocyte maturation

2001 ◽  
Vol 93 (1-2) ◽  
pp. 15-25 ◽  
Author(s):  
Anna Castro ◽  
Marion Peter ◽  
Thierry Lorca ◽  
Elisabeth Mandart
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte ◽  
Cyclin B

scholarly journals Regulation of Src kinase activity during Xenopus oocyte maturation

2005 ◽  
Vol 278 (2) ◽  
pp. 289-300 ◽  
Author(s):  
Alexander Tokmakov ◽  
Tetsushi Iwasaki ◽  
Shuji Itakura ◽  
Ken-Ichi Sato ◽  
Mikako Shirouzu ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte ◽  
Kinase Activity ◽  
Src Kinase

In vitro facilitation of Xenopus oocyte maturation by subthreshold doses of progesterone

1977 ◽  
Vol 59 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Jean Marot ◽  
Robert Bellé ◽  
René Ozon
Keyword(s):  
Oocyte Maturation ◽  
Xenopus Oocyte

scholarly journals Mechanism and cellular function of Bud6 as an actin nucleation–promoting factor

2011 ◽  
Vol 22 (21) ◽  
pp. 4016-4028 ◽  
Author(s):  
Brian R. Graziano ◽  
Amy Grace DuPage ◽  
Alphee Michelot ◽  
Dennis Breitsprecher ◽  
James B. Moseley ◽  
...  
Keyword(s):  
Actin Assembly ◽  
Actin Cable ◽  
Polarized Cell Growth ◽  
Cable Assembly ◽  
Nucleation Promoting Factor ◽  
Actin Cables ◽  
Elongation Phase

Formins are a conserved family of actin assembly–promoting factors with diverse biological roles, but how their activities are regulated in vivo is not well understood. In Saccharomyces cerevisiae, the formins Bni1 and Bnr1 are required for the assembly of actin cables and polarized cell growth. Proper cable assembly further requires Bud6. Previously it was shown that Bud6 enhances Bni1-mediated actin assembly in vitro, but the biochemical mechanism and in vivo role of this activity were left unclear. Here we demonstrate that Bud6 specifically stimulates the nucleation rather than the elongation phase of Bni1-mediated actin assembly, defining Bud6 as a nucleation-promoting factor (NPF) and distinguishing its effects from those of profilin. We generated alleles of Bud6 that uncouple its interactions with Bni1 and G-actin and found that both interactions are critical for NPF activity. Our data indicate that Bud6 promotes filament nucleation by recruiting actin monomers to Bni1. Genetic analysis of the same alleles showed that Bud6 regulation of formin activity is critical for normal levels of actin cable assembly in vivo. Our results raise important mechanistic parallels between Bud6 and WASP, as well as between Bud6 and other NPFs that interact with formins such as Spire.

scholarly journals Reorganization of actin filaments by ADF/cofilin is involved in formation of microtubule structures during Xenopus oocyte maturation

2015 ◽  
Vol 26 (24) ◽  
pp. 4387-4400 ◽  
Author(s):  
Yuka Yamagishi ◽  
Hiroshi Abe
Keyword(s):  
Oocyte Maturation ◽  
Actin Filament ◽  
Actin Filaments ◽  
Xenopus Oocyte ◽  
Germinal Vesicle ◽  
Meiotic Spindle ◽  
Base Region ◽  
Microtubule Organizing Center ◽  
Cytoplasmic Actin ◽  
Organizing Center

We examined the reorganization of actin filaments and microtubules during Xenopus oocyte maturation. Surrounding the germinal vesicle (GV) in immature oocytes, the cytoplasmic actin filaments reorganized to accumulate beneath the vegetal side of the GV, where the microtubule-organizing center and transient microtubule array (MTOC-TMA) assembled, just before GV breakdown (GVBD). Immediately after GVBD, both Xenopus ADF/cofilin (XAC) and its phosphatase Slingshot (XSSH) accumulated into the nuclei and intranuclear actin filaments disassembled from the vegetal side with the shrinkage of the GV. As the MTOC-TMA developed well, cytoplasmic actin filaments were retained at the MTOC-TMA base region. Suppression of XAC dephosphorylation by anti-XSSH antibody injection inhibited both actin filament reorganization and proper formation and localization of both the MTOC-TMA and meiotic spindles. Stabilization of actin filaments by phalloidin also inhibited formation of the MTOC-TMA and disassembly of intranuclear actin filaments without affecting nuclear shrinkage. Nocodazole also caused the MTOC-TMA and the cytoplasmic actin filaments at its base region to disappear, which further impeded disassembly of intranuclear actin filaments from the vegetal side. XAC appears to reorganize cytoplasmic actin filaments required for precise assembly of the MTOC and, together with the MTOC-TMA, regulate the intranuclear actin filament disassembly essential for meiotic spindle formation.

The mechanism for increased protein synthesis during Xenopus oocyte maturation

1982 ◽  
Vol 89 (1) ◽  
pp. 159-167 ◽  
Author(s):  
Joel D. Richter ◽  
William J. Wasserman ◽  
L.Dennis Smith
Keyword(s):  
Protein Synthesis ◽  
Oocyte Maturation ◽  
Xenopus Oocyte
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