Characterization of myosin II regulatory light chain isoforms in HeLa cells

Cytoskeleton ◽  
2015 ◽  
Vol 72 (12) ◽  
pp. 609-620 ◽  
Author(s):  
Tomo Kondo ◽  
Morihiro Okada ◽  
Kayo Kunihiro ◽  
Masayuki Takahashi ◽  
Yoshio Yaoita ◽  
...  
Keyword(s):  
Light Chain ◽  
Hela Cells ◽  
Myosin Ii ◽  
Regulatory Light Chain

scholarly journals CONCENTRATION OF SINGLY PHOSPHORYLATED MYOSIN II REGULATORY LIGHT CHAIN ALONG THE CLEAVAGE FURROW OF DIVIDING HeLa CELLS

1998 ◽  
Vol 19 (2) ◽  
pp. 111-115 ◽  
Author(s):  
MAKI MURATA-HORI ◽  
NORIO MURAI ◽  
SATOSHI KOMATSU ◽  
YOSHIE UJI ◽  
HIROSHI HOSOYA
Keyword(s):  
Light Chain ◽  
Hela Cells ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Cleavage Furrow

scholarly journals Phosphorylation of myosin II regulatory light chain is necessary for migration of HeLa cells but not for localization of myosin II at the leading edge

2003 ◽  
Vol 370 (2) ◽  
pp. 551-556 ◽  
Author(s):  
Katsumi FUMOTO ◽  
Takashi UCHIMURA ◽  
Takahiro IWASAKI ◽  
Kozue UEDA ◽  
Hiroshi HOSOYA
Keyword(s):  
Light Chain ◽  
Hela Cells ◽  
Myosin Ii ◽  
Leading Edge ◽  
Regulatory Light Chain ◽  
Wild Type ◽  
Wound Healing Assay ◽  
Cell Monolayers ◽  
Indirect Immunofluorescence Staining

To investigate the role of phosphorylated myosin II regulatory light chain (MRLC) in living cell migration, these mutant MRLCs were engineered and introduced into HeLa cells. The mutant MRLCs include an unphosphorylatable form, in which both Thr-18 and Ser-19 were substituted with Ala (AA-MRLC), and pseudophosphorylated forms, in which Thr-18 and Ser-19 were replaced with Ala and Asp, respectively (AD-MRLC), and both Thr-18 and Ser-19 were replaced with Asp (DD-MRLC). Mutant MRLC-expressing cell monolayers were mechanically stimulated by scratching, and the cells were forced to migrate in a given direction. In this wound-healing assay, the AA-MRLC-expressing cells migrated much more slowly than the wild-type MRLC-expressing cells. In the case of DD-MRLC- and AD-MRLC-expressing cells, no significant differences compared with wild-type MRLC-expressing cells were observed in their migration speed. Indirect immunofluorescence staining showed that the accumulation of endogenous diphosphorylated MRLC at the leading edge was not observed in AA-MRLC-expressing cells, although AA-MRLC was incorporated into myosin heavy chain and localized at the leading edge. In conclusion, we propose that the phosphorylation of MRLC is required to generate the driving force in the migration of the cells but not necessary for localization of myosin II at the leading edge.

scholarly journals Spatial Localization of Mono-and Diphosphorylated Myosin II Regulatory Light Chain at the Leading Edge of Motile HeLa Cells

2002 ◽  
Vol 27 (6) ◽  
pp. 479-486 ◽  
Author(s):  
Takashi Uchimura ◽  
Katsumi Fumoto ◽  
Yoshihiro Yamamoto ◽  
Kozue Ueda ◽  
Hiroshi Hosoya
Keyword(s):  
Light Chain ◽  
Hela Cells ◽  
Myosin Ii ◽  
Leading Edge ◽  
Spatial Localization ◽  
Regulatory Light Chain

Adenosine induces dephosphorylation of myosin II regulatory light chain in cultured bovine corneal endothelial cells

2004 ◽  
Vol 79 (4) ◽  
pp. 543-551 ◽  
Author(s):  
S.P. Srinivas ◽  
M. Satpathy ◽  
P. Gallagher ◽  
E. Larivière ◽  
W. Van Driessche
Keyword(s):  
Endothelial Cells ◽  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Corneal Endothelial Cells

Telokin/KRP differentially modulates myosin II filament assembly and regulatory light chain phosphorylation in fibroblasts

BIOPHYSICS ◽  
2006 ◽  
Vol 51 (5) ◽  
pp. 764-770
Author(s):  
D. V. Serebryanaya ◽  
O. V. Shcherbakova ◽  
T. V. Dudnakova ◽  
V. P. Shirinsky ◽  
A. V. Vorotnikov
Keyword(s):  
Light Chain ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Filament Assembly ◽  
Regulatory Light Chain Phosphorylation

scholarly journals Effects of the Regulatory Light Chain Phosphorylation of Myosin II on Mitosis and Cytokinesis of Mammalian Cells

2000 ◽  
Vol 275 (44) ◽  
pp. 34512-34520 ◽  
Author(s):  
Satoshi Komatsu ◽  
Takeo Yano ◽  
Masao Shibata ◽  
Richard A. Tuft ◽  
Mitsuo Ikebe
Keyword(s):  
Light Chain ◽  
Mammalian Cells ◽  
Myosin Ii ◽  
Regulatory Light Chain ◽  
Regulatory Light Chain Phosphorylation
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