Novel application of flow cytometry: Determination of muscle fiber types and protein levels in whole murine skeletal muscles and heart

2007 ◽  
Vol 64 (12) ◽  
pp. 914-925 ◽  
Author(s):  
Connie Jackaman ◽  
Kristen J. Nowak ◽  
Gianina Ravenscroft ◽  
Esther M. Lim ◽  
Sophie Clément ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Muscle Fiber ◽  
Skeletal Muscles ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Protein Levels

Effects of Co60 Irradiation on Muscle Fiber Types of the Grass Frog

2000 ◽  
Vol 6 (S2) ◽  
pp. 852-853
Author(s):  
Glenn M. Cohen ◽  
Margaret F. Scott
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Striated Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Mitochondrial Enzymes ◽  
Metabolic Characteristics ◽  
Long Latency ◽  
Low Levels

Striated skeletal muscle has been considered radioresistant because it is highly differentiated and post-mitotic. Striated muscle does, however, respond to irradiation with morphological and biochemical changes after short and long latency periods; vascular and/or neurological impairments might contribute to the delayed responses to irradiation.The objective of the present study was to determine the susceptibility of three amphibian muscle fiber types to Co60 irradiation. In amphibians, the three major fiber types are 1) large twitch fibers, which contain low levels of mitochondrial enzymes and lipids, but intermediate levels of glycogen; 2) small twitch fibers, which contain high levels of both glycolytic and mitochondrial enzymes (FIG. 1); and tonic fibers, which contain low levels of all three histochemical markers. Thus, the determination of susceptibility of different amphibian fiber types to irradiation might indicate whether the metabolic characteristics of the fibers, rather than morphological or electrical properties, could serve as an early indicator of radiation damage.

Relationship between muscle fiber characteristics and meat quality parameters in Turkish native goat breeds

2018 ◽  
Author(s):  
Emre Sirin
Keyword(s):  
Meat Quality ◽  
Muscle Fiber ◽  
Quality Parameters ◽  
Muscle Fiber Types ◽  
Type I ◽  
Fiber Types ◽  
Fiber Number ◽  
Weaning Age ◽  
Positive Correlations

This study was conducted to determine muscle fiber characteristics and their effect on some meat quality parameters in Longissimus dorsi (LD) and Semitendinosus (ST) muscles from kids of some Turkish native goat breeds. Male kids of Hair (n=6), Angora (n=6), Kilis (n=6) and Honamli (n=6) (pure breeds) were used as experimental animals. All kids were slaughtered at 3 months of weaning age and muscles samples were collected for determination of type I, IIA and IIB muscle fibers and some meat quality parameters. It was found that type IIA fiber number of Hair and Honamli kids were higher than those of other breeds in LD muscle. Similarly, Hair kids had higher number of (P less than 0.05) type IIA in ST muscle compared to other breeds. Generally, there were negative correlations between tenderness, pH and number of muscle fiber types in LD and ST muscles of all breeds (P less than0.05). Also, there were positive correlations between intra muscular fat and number of muscle fiber types in LD muscles of all breeds (P less than 0.05). Conclusively, kids of Turkish native goat breeds had different muscle fiber characteristics which can affect meat quality.

NON-INVASIVE DETERMINATION OF HUMAN MUSCLE FIBER TYPES

1983 ◽  
Vol 15 (2) ◽  
pp. 105
Author(s):  
T. Moritani ◽  
F. A. Gaffney ◽  
T. Carmichael
Keyword(s):  
Muscle Fiber ◽  
Human Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Non Invasive

Variation in the distribution of muscle fiber types in equine skeletal muscles

1985 ◽  
Vol 5 (1) ◽  
pp. 34-37 ◽  
Author(s):  
Randel R. Raub ◽  
Peter J. Bechtel ◽  
Laurie M. Lawrence
Keyword(s):  
Muscle Fiber ◽  
Skeletal Muscles ◽  
Muscle Fiber Types ◽  
Fiber Types
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