Caldesmon is necessary for maintaining the actin and intermediate filaments in cultured bladder smooth muscle cells

2007 ◽  
Vol 64 (12) ◽  
pp. 951-965 ◽  
Author(s):  
Maoxian Deng ◽  
Sunish Mohanan ◽  
Erzsebet Polyak ◽  
Samuel Chacko
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Intermediate Filaments ◽  
Muscle Cells ◽  
Bladder Smooth Muscle

1708: Transfection of Bladder Smooth Muscle Cells with Telokin Alters their Smooth Muscle Phenotype

2004 ◽  
Vol 171 (4S) ◽  
pp. 452-452
Author(s):  
Grace Hyun ◽  
Shaohua Chang ◽  
Stephen A. Zderic ◽  
Alan J. Wein ◽  
Samuel Chacko ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bladder Smooth Muscle

scholarly journals Stretch-induced Calcium Release in Smooth Muscle

2002 ◽  
Vol 119 (6) ◽  
pp. 533-543 ◽  
Author(s):  
Guangju Ji ◽  
Robert J. Barsotti ◽  
Morris E. Feldman ◽  
Michael I. Kotlikoff
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Calcium Release ◽  
Muscle Cells ◽  
Intraluminal Pressure ◽  
Bladder Smooth Muscle ◽  
Urinary Bladder Smooth Muscle ◽  
Inward Currents ◽  
Longitudinal Stretch

Smooth muscle cells undergo substantial increases in length, passively stretching during increases in intraluminal pressure in vessels and hollow organs. Active contractile responses to counteract increased transmural pressure were first described almost a century ago (Bayliss, 1902) and several mechanisms have been advanced to explain this phenomenon. We report here that elongation of smooth muscle cells results in ryanodine receptor–mediated Ca2+ release in individual myocytes. Mechanical elongation of isolated, single urinary bladder myocytes to ∼120% of slack length (ΔL = 20) evoked Ca2+ release from intracellular stores in the form of single Ca2+ sparks and propagated Ca2+ waves. Ca2+ release was not due to calcium-induced calcium release, as release was observed in Ca2+-free extracellular solution and when free Ca2+ ions in the cytosol were strongly buffered to prevent increases in [Ca2+]i. Stretch-induced calcium release (SICR) was not affected by inhibition of InsP3R-mediated Ca2+ release, but was completely blocked by ryanodine. Release occurred in the absence of previously reported stretch-activated currents; however, SICR evoked calcium-activated chloride currents in the form of transient inward currents, suggesting a regulatory mechanism for the generation of spontaneous currents in smooth muscle. SICR was also observed in individual myocytes during stretch of intact urinary bladder smooth muscle segments. Thus, longitudinal stretch of smooth muscle cells induces Ca2+ release through gating of RYR. SICR may be an important component of the physiological response to increases in luminal pressure in smooth muscle tissues.

The Role of Signal Transducer and Activator of Transcription 3 (Stat3) in Stretch Injury to Bladder Smooth Muscle Cells

2007 ◽  
Vol 3 ◽  
pp. S35
Author(s):  
Sarel Halachmi ◽  
Karen Aitken ◽  
Martha Szybowska ◽  
Nesrin Sabha ◽  
Shariff Dessouki ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Signal Transducer ◽  
Bladder Smooth Muscle ◽  
Stretch Injury ◽  
Transcription 3

Growth and stretch response of human exstrophy bladder smooth muscle cells: molecular evidence of normal intrinsic function

2005 ◽  
Vol 95 (1) ◽  
pp. 144-148 ◽  
Author(s):  
Anna Orsola ◽  
Carlos R. Estrada ◽  
Hiep T. Nguyen ◽  
Alan B. Retik ◽  
Michael R. Freeman ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Molecular Evidence ◽  
Bladder Smooth Muscle ◽  
Stretch Response ◽  
Intrinsic Function
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