Differential organelle movement on the actin cytoskeleton in lily pollen tubes

2007 ◽  
Vol 64 (3) ◽  
pp. 217-232 ◽  
Author(s):  
Alenka Lovy-Wheeler ◽  
Luis Cárdenas ◽  
Joseph G. Kunkel ◽  
Peter K. Hepler
Keyword(s):  
Actin Cytoskeleton ◽  
Pollen Tubes ◽  
Organelle Movement ◽  
Lily Pollen

scholarly journals Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

2021 ◽  
Vol 40 (2) ◽  
pp. 205-222
Author(s):  
Monica Scali ◽  
Alessandra Moscatelli ◽  
Luca Bini ◽  
Elisabetta Onelli ◽  
Rita Vignani ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Membrane Trafficking ◽  
Tip Growth ◽  
Male Gametophyte ◽  
Pollen Tubes ◽  
Structural Features ◽  
Two Dimensional Gel Electrophoresis ◽  
Depth Analysis

AbstractPollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identifyNicotiana tabacumDifferentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

scholarly journals NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth

2021 ◽  
Author(s):  
Patrick Duckney ◽  
Johan T. Kroon ◽  
Martin R. Dixon ◽  
Timothy J. Hawkins ◽  
Michael J. Deeks ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Cortical Actin

Fine structure of Lily pollen tubes following various fixation and staining procedures

PROTOPLASMA ◽  
1966 ◽  
Vol 61 (1-2) ◽  
pp. 181-191 ◽  
Author(s):  
Walter G. Rosen ◽  
Stanley R. Gawlik
Keyword(s):  
Fine Structure ◽  
Pollen Tubes ◽  
Lily Pollen

scholarly journals Alterations in the Actin Cytoskeleton of Pollen Tubes Are Induced by the Self-Incompatibility Reaction in Papaver rhoeas

2000 ◽  
Vol 12 (7) ◽  
pp. 1239-1251 ◽  
Author(s):  
Anja Geitmann ◽  
Benjamin N. Snowman ◽  
Anne Mie C. Emons ◽  
Vernonica E. Franklin-Tong
Keyword(s):  
Actin Cytoskeleton ◽  
Pollen Tubes ◽  
The Self ◽  
Self Incompatibility ◽  
Papaver Rhoeas ◽  
Incompatibility Reaction

scholarly journals Interactive computer-assisted position acquisition procedure designed for the analysis of organelle movement in pollen tubes

Cytometry ◽  
1998 ◽  
Vol 32 (4) ◽  
pp. 263-267 ◽  
Author(s):  
A.H.N. de Win ◽  
E.S. Pierson ◽  
C. Timmer ◽  
I.K. Lichtscheidl ◽  
J. Derksen
Keyword(s):  
Pollen Tubes ◽  
Computer Assisted ◽  
Organelle Movement

scholarly journals Organelle movement and apical accumulation of secretory vesicles in pollen tubes of Arabidopsis thaliana depend on class XI myosins

2020 ◽  
Vol 104 (6) ◽  
pp. 1685-1697
Author(s):  
Xingjuan Wang ◽  
Xiaojing Sheng ◽  
Xiulin Tian ◽  
Yu Zhang ◽  
Yan Li
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tubes ◽  
Secretory Vesicles ◽  
Organelle Movement

scholarly journals Molecular Cloning of Two Exo-β-glucanases and Their in vivo Substrates in the Cell Walls of Lily Pollen Tubes

2004 ◽  
Vol 45 (4) ◽  
pp. 436-444 ◽  
Author(s):  
Hiroyuki Takeda ◽  
Takuo Yoshikawa ◽  
Xi-Zhen Liu ◽  
Naoki Nakagawa ◽  
Yi-Qin Li ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Cell Walls ◽  
Pollen Tubes ◽  
Lily Pollen

scholarly journals Accelerated sliding of pollen tube organelles along Characeae actin bundles regulated by Ca2+.

1988 ◽  
Vol 106 (5) ◽  
pp. 1539-1543 ◽  
Author(s):  
T Kohno ◽  
T Shimmen
Keyword(s):  
Heat Treatment ◽  
Pollen Tube ◽  
Cytoplasmic Streaming ◽  
Pollen Tubes ◽  
The Other ◽  
Cell Models ◽  
Organelle Movement ◽  
Actin Bundles ◽  
Other Hand ◽  
Order Of Magnitude

Pollen tubes show active cytoplasmic streaming. We isolated organelles from pollen tubes and tested their ability to slide along actin bundles in characean cell models. Here, we show that sliding of organelles was ATP-dependent and that motility was lost after N-ethylmaleimide or heat treatment of organelles. On the other hand, cytoplasmic streaming in pollen tube was inhibited by either N-ethylmaleimide or heat treatment. These results strongly indicate that cytoplasmic streaming in pollen tubes is supported by the "actomyosin"-ATP system. The velocity of organelle movement along characean actin bundles was much higher than that of the native streaming in pollen tubes. We suggested that pollen tube "myosin" has a capacity to move at a velocity of the same order of magnitude as that of characean myosin. Moreover, the motility was high at Ca2+ concentrations lower than 0.18 microM (pCa 6.8) but was inhibited at concentration higher than 4.5 microM (pCa 5.4). In conclusion, cytoplasmic streaming in pollen tubes is suggested to be regulated by Ca2+ through "myosin" inactivation.

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