Differential expression and localization of neuronal intermediate filament proteins within newly developing neurites in dissociated cultures ofXenopus laevis embryonic spinal cord

2001 ◽  
Vol 49 (1) ◽  
pp. 16-32 ◽  
Author(s):  
Jayanthi Undamatla ◽  
Ben G. Szaro
Keyword(s):  
Spinal Cord ◽  
Differential Expression ◽  
Intermediate Filament ◽  
Intermediate Filament Proteins ◽  
Embryonic Spinal Cord ◽  
Neuronal Intermediate Filament Proteins

scholarly journals Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF‐M/H) in Alzheimer's disease by iTRAQ

2010 ◽  
Vol 24 (11) ◽  
pp. 4396-4407 ◽  
Author(s):  
Parvathi Rudrabhatla ◽  
Philip Grant ◽  
Howard Jaffe ◽  
Michael J. Strong ◽  
Harish C. Pant
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Intermediate Filament ◽  
Intermediate Filament Proteins ◽  
Neuronal Intermediate Filament Proteins

scholarly journals Characterisation of neurofilament protein NF70 mRNA from the gastropod Helix aspersa reveals that neuronal and non-neuronal intermediate filament proteins of cerebral ganglia arise from separate lamin-related genes

1995 ◽  
Vol 108 (11) ◽  
pp. 3581-3590 ◽  
Author(s):  
J. Adjaye ◽  
U. Plessmann ◽  
K. Weber ◽  
H. Dodemont
Keyword(s):  
Sequence Analysis ◽  
Intermediate Filament ◽  
Cerebral Ganglion ◽  
Helix Aspersa ◽  
Neurofilament Protein ◽  
Intermediate Filament Proteins ◽  
Nuclear Lamins ◽  
Untranslated Sequence ◽  
Cerebral Ganglia ◽  
Neuronal Intermediate Filament Proteins

The neuronal cytoplasmic intermediate filament (IF) protein HeNF70 of the gastropod Helix aspersa is identified by sequence analysis of the corresponding 4,600 bp cDNA isolated from a cerebral ganglion cDNA library. HeNF70 shares 60% sequence identity with the neuronal LoNF70 protein of the cephalopod Loligo pealei and only 36% identity with the Helix non-neuronal IF-A protein. All three molluscan IF proteins display the lamin-type extended coil 1b subdomain harbouring six additional heptads and all have long C-terminal sequences with substantial homology to the tail domains of nuclear lamins. The lamin-like tail domains of the two neurofilament proteins share a unique motif comprising 13 residues, which is absent from Helix IF-A and all other known non-neuronal IF proteins. HeNF70 is encoded by a 9.5 kb RNA transcript. The very long 7.2 kb 3′-untranslated sequence contains a unique 26 nucleotide repeat extending over 0.5 kb in its 5′-region. The HeNF70 mRNA is expressed at low abundancy in cerebral ganglia but not in any of the 13 non-neuronal tissues tested. In contrast, all tissues express at fairly high levels the same 4.6 and 4.2 kb mRNAs encoding the Helix non-neuronal IF-A/B proteins. Blot hybridisation studies on genomic DNA and ganglion mRNA with subprobes from the cloned HeNF70 cDNA, as well as sequence analysis of an RT-PCR generated partial cDNA encoding a putative HeNF60 protein, indicate at least two different neuronal IF genes in Helix.

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