Genomic organization, alternative splicing, and expression of human and mouseN-RAP, a nebulin-related LIM protein of striated muscle

2003 ◽  
Vol 55 (3) ◽  
pp. 200-212 ◽  
Author(s):  
Saidi A. Mohiddin ◽  
Shajia Lu ◽  
John-Paul Cardoso ◽  
Stefanie Carroll ◽  
Sanjaya Jha ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Genomic Organization ◽  
Striated Muscle ◽  
Lim Protein

Alternative splicing of PDLIM3/ALP, for α-actinin-associated LIM protein 3, is aberrant in persons with myotonic dystrophy

2011 ◽  
Vol 409 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Natsumi Ohsawa ◽  
Michinori Koebis ◽  
Satoshi Suo ◽  
Ichizo Nishino ◽  
Shoichi Ishiura
Keyword(s):  
Alternative Splicing ◽  
Myotonic Dystrophy ◽  
Lim Protein

Genomic organization, expression of the human CBFA1 gene, and evidence for an alternative splicing event affecting protein function

1998 ◽  
Vol 9 (1) ◽  
pp. 54-57 ◽  
Author(s):  
V. Geoffroy ◽  
D. A. Corral ◽  
L. Zhou ◽  
B. Lee ◽  
G. Karsenty
Keyword(s):  
Alternative Splicing ◽  
Protein Function ◽  
Genomic Organization

scholarly journals Erratum to: Genomic organization and alternative splicing of the human and mouse RPTPρ genes: Correction

BMC Genomics ◽  
2001 ◽  
Vol 2 (1) ◽  
Author(s):  
Julie A Besco ◽  
Adrienne Frostholm ◽  
Magdalena C Popesco ◽  
Arthur HM Burghes ◽  
Andrej Rotter
Keyword(s):  
Alternative Splicing ◽  
Genomic Organization ◽  
Human And Mouse

scholarly journals The CELF Family of RNA Binding Proteins Is Implicated in Cell-Specific and Developmentally Regulated Alternative Splicing

2001 ◽  
Vol 21 (4) ◽  
pp. 1285-1296 ◽  
Author(s):  
Andrea N. Ladd ◽  
Nicolas Charlet-B. ◽  
Thomas A. Cooper
Keyword(s):  
Alternative Splicing ◽  
Heart Development ◽  
Binding Proteins ◽  
Rna Binding ◽  
Striated Muscle ◽  
Rna Binding Proteins ◽  
Binding Protein ◽  
Protein Isoforms ◽  
Developmentally Regulated ◽  
Exon Inclusion

ABSTRACT Alternative splicing of cardiac troponin T (cTNT) exon 5 undergoes a developmentally regulated switch such that exon inclusion predominates in embryonic, but not adult, striated muscle. We previously described four muscle-specific splicing enhancers (MSEs) within introns flanking exon 5 in chicken cTNT that are both necessary and sufficient for exon inclusion in embryonic muscle. We also demonstrated that CUG-binding protein (CUG-BP) binds a conserved CUG motif within a human cTNT MSE and positively regulates MSE-dependent exon inclusion. Here we report that CUG-BP is one of a novel family of developmentally regulated RNA binding proteins that includes embryonically lethal abnormal vision-type RNA binding protein 3 (ETR-3). This family, which we call CELF proteins for CUG-BP- and ETR-3-like factors, specifically bound MSE-containing RNAs in vitro and activated MSE-dependent exon inclusion of cTNT minigenes in vivo. The expression of two CELF proteins is highly restricted to brain. CUG-BP, ETR-3, and CELF4 are more broadly expressed, and expression is developmentally regulated in striated muscle and brain. Changes in the level of expression and isoforms of ETR-3 in two different developmental systems correlated with regulated changes in cTNT splicing. A switch from cTNT exon skipping to inclusion tightly correlated with induction of ETR-3 protein expression during differentiation of C2C12 myoblasts. During heart development, the switch in cTNT splicing correlated with a transition in ETR-3 protein isoforms. We propose that ETR-3 is a major regulator of cTNT alternative splicing and that the CELF family plays an important regulatory role in cell-specific alternative splicing during normal development and disease.

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