scholarly journals En route to personalized medicine: uncovering distinct IgE reactivity pattern to house dust mite components in Brazilian and Austrian allergic patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carina S. Pinheiro ◽  
Eduardo S. Silva ◽  
Emília M. M. de Andrade Belitardo ◽  
Luis G. C. Pacheco ◽  
Eric R. G. R. Aguiar ◽  
...  
Keyword(s):  
Personalized Medicine ◽  
House Dust ◽  
House Dust Mite ◽  
Reactivity Pattern ◽  
Ige Reactivity ◽  
Dust Mite

The allergen-specific IgE reactivity pattern of Chinese house dust mite allergic patients

2007 ◽  
Vol &NA; ◽  
pp. S262
Author(s):  
Chunqing Zhang ◽  
Birgitte Gjesing ◽  
Henrik Ipsen ◽  
Helene Henmar ◽  
Michael Spangfort ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Specific Ige ◽  
Reactivity Pattern ◽  
Ige Reactivity ◽  
Dust Mite

The allergen-specific IgE reactivity pattern of Chinese house dust mite allergic patients

Allergy ◽  
2008 ◽  
Vol 63 (12) ◽  
pp. 1640-1641 ◽  
Author(s):  
C. Zhang ◽  
B. Gjesing ◽  
M. D. Spangfort ◽  
J. Xu ◽  
N. Zhong
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Specific Ige ◽  
Reactivity Pattern ◽  
Ige Reactivity ◽  
Dust Mite

scholarly journals Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus

2009 ◽  
Vol 74 (5) ◽  
pp. 513-522 ◽  
Author(s):  
Katarina Milovanovic ◽  
Lidija Burazer ◽  
Olga Vuckovic ◽  
Marina Atanaskovic-Markovic ◽  
Tanja Cirkovic-Velickovic ◽  
...  
Keyword(s):  
Molecular Mass ◽  
House Dust ◽  
House Dust Mite ◽  
Blot Analysis ◽  
High Molecular Mass ◽  
House Dust Mites ◽  
House Dust Mite Allergy ◽  
Ige Reactivity ◽  
Mite Allergy ◽  
Dust Mite

House dust mites (HDM) represent a major source of allergens, contributing to the increasing incidence of type I hypersensitivity disease worldwide. Over 30 different IgE-binding proteins from the HDM extract were detected. Although group 1 and 2 have been identified as major allergens, due to the safety and efficacy of allergy diagnosis and immunotherapy, there is a need to carefully evaluate the clinical relevance of other allergens present in the HDM extract. In regard to this, a high molecular mass allergen of about 68 kD was purified from the HDM extract using a combination of gel permeation chromatography and reversed-phase chromatography. The IgG and IgE reactivity of the purified protein were preserved during the purification process, as confirmed by Western blot analysis with polyclonal rabbit antibodies and dot blot analysis with a pool of sera from subjects with house dust mite allergy, respectively. In addition, the IgE reactivity was confirmed using ELISA testing with nine patient sera. The biological potency of the 68 kD allergen was confirmed by skin prick testing in five allergic subjects, suggesting that the high molecular mass allergen is a good candidate for component-resolved diagnosis of house dust mite allergy and eventual therapeutic treatment.

IgE reactivity profiles among house dust mite allergic patients in Bavaria

2013 ◽  
Vol 270 (12) ◽  
pp. 3177-3182 ◽  
Author(s):  
Sven Becker ◽  
Matthias F. Kramer ◽  
Miriam Havel ◽  
Christian Welz ◽  
Sabine Markmann ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Ige Reactivity ◽  
Dust Mite

scholarly journals IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational

2021 ◽  
Vol 12 ◽  
Author(s):  
Mirela Curin ◽  
Huey-Jy Huang ◽  
Tetiana Garmatiuk ◽  
Sandra Gutfreund ◽  
Yvonne Resch-Marat ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Basophil Activation ◽  
House Dust Mite Allergen ◽  
Ige Reactivity ◽  
Activation Assay ◽  
Dust Mite ◽  
Allergenic Activity ◽  
Specific Igg ◽  
Specific Igg Antibodies

BackgroundSeveral studies indicate that Der p 7 is an important and clinically relevant allergen of Dermatophagoides pteronyssinus which should be included in vaccines for treatment of house dust mite (HDM) allergy. Aim of this study was to characterize the IgE epitopes of Der p 7.MethodsRecombinant Der p 7 was expressed and purified, analyzed for fold by circular dichroism and tested for its allergenic activity by basophil activation. Seven overlapping, surface-exposed peptides (P1–P7) with a length of 27 to 37 amino acids, which spanned the Der p 7 sequence, were synthesized and tested for IgE reactivity and allergenic activity by basophil activation assay. Carrier-bound peptides were studied for their ability to induce allergen-specific IgG antibodies in rabbits. Peptide-specific antibodies were used to inhibit allergic patients` IgE binding to Der p 7 by ELISA for mapping of IgE epitopes.ResultsrDer p 7 showed high allergenic activity comparable with Der p 5, Der p 21, and Der p 23. None of the seven tested peptides showed any IgE reactivity or allergenic activity when tested with HDM- allergic patients indicating lack of sequential IgE epitopes on Der p 7. IgE inhibition experiments using anti-peptide specific IgGs and molecular modeling enabled us to identify discontinuous, conformational IgE epitopes of Der p 7.Conclusion and Clinical RelevanceIgE epitopes of Der p 7 belong to the conformational and discontinuous type whereas sequential Der p 7 peptides lack IgE reactivity. It should thus be possible to construct hypoallergenic vaccines for Der p 7 based on carrier-bound allergen peptides.

scholarly journals IgE reactivity to shrimp allergens in infants and their cross-reactivity to house dust mite

2017 ◽  
Vol 28 (7) ◽  
pp. 703-707 ◽  
Author(s):  
Sandip D. Kamath ◽  
Elecia B. Johnston ◽  
Swati Iyer ◽  
Patrick M. Schaeffer ◽  
Jennifer Koplin ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Cross Reactivity ◽  
Ige Reactivity ◽  
Dust Mite

Specific IgE reactivity to fourteen recombinant allergens from house dust mite, Blomia tropicalis

2002 ◽  
Vol 109 (1) ◽  
pp. S207-S207
Author(s):  
Wl Wang ◽  
KT Kuay ◽  
HS Shang ◽  
BL Lua ◽  
Bee Wah Lee ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Specific Ige ◽  
Recombinant Allergens ◽  
Blomia Tropicalis ◽  
Ige Reactivity ◽  
Dust Mite

Heterogeneous proteolytic specificity and activity of the house dust mite proteinase allergen Der p I

1997 ◽  
Vol 27 (2) ◽  
pp. 202-207 ◽  
Author(s):  
C. R. A. HEWITT ◽  
H. HORTON ◽  
R. M. JONES ◽  
D. I. PRITCHARD
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Dust Mite

Characterization of lipophilic house dust mite-allergens with regard to the allergic phenotype

Pneumologie ◽  
2014 ◽  
Vol 68 (06) ◽  
Author(s):  
S Kull ◽  
A Petersen ◽  
S Vrtala ◽  
U Jappe
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Dust Mite
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