Synthese des Glykosids 2 ( β ‐Galaktosyl)‐Ethyl Methacrylat mithilfe einer β Galaktosidase aus Pyrococcus woesei für die Glykopolymersynthese

2020 ◽  
Vol 92 (9) ◽  
pp. 1226-1227
Author(s):  
C. Wahl ◽  
M. Hoffmann ◽  
E. Gau ◽  
S. Braun ◽  
A. Pich ◽  
...  
Keyword(s):  
1998 ◽  
Vol 54 (6) ◽  
pp. 1419-1421 ◽  
Author(s):  
Graeme S. Bell ◽  
Rupert J. M. Russell ◽  
Michael Kohlhoff ◽  
Reinhard Hensel ◽  
Michael J. Danson ◽  
...  

Recombinant triosephosphate isomerase (TIM) from a hyperthermophilic Archaeon, Pyrococcus woesei, has been crystallized. Three crystal forms have been obtained: monoclinic, orthorhombic and hexagonal. The monoclinic crystals belong to space group P21 with cell dimensions a = 79.1, b = 89.2, c = 145.4 Å and β = 92.8°, and diffract to at least 2.6 Å. The orthorhombic crystals belong to space group P21212 with a = 89.4, b = 155.9, c = 79.5 Å, and diffract to 2.9 Å. Diffraction from the hexagonal form showed extensive disorder. The monoclinic form contains two tetramers in the asymmetric unit, which are in the same orientation but related by a pseudo-centring. The orthorhombic form contains one tetramer in the asymmetric unit which is in approximately the same orientation as in the monoclinic form. Knowledge of the structure of this hyperthermostable TIM, which is tetrameric in contrast to dimeric forms previously observed, will add to the understanding of protein thermostability.


1991 ◽  
Vol 155 (6) ◽  
pp. 572-578 ◽  
Author(s):  
Rainhard Koch ◽  
Andreas Spreinat ◽  
Karen Lemke ◽  
Garabed Antranikian

1990 ◽  
Vol 70 (3) ◽  
pp. 285-290
Author(s):  
M. Valeria Catani ◽  
Sergio Altamura ◽  
Paola Londei

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