The µRAMOS: A tool for high‐throughput online monitoring of respiration activities in 96‐deepwell microtiter plates

2020 ◽  
Vol 92 (9) ◽  
pp. 1208-1208
Author(s):  
R. Dinger ◽  
C. Lattermann ◽  
D. Flitsch ◽  
J. Büchs
Keyword(s):  
High Throughput ◽  
Online Monitoring ◽  
Microtiter Plates

scholarly journals High-Throughput Peptide Derivatization toward Supramolecular Diversification in Microtiter Plates

ACS Nano ◽  
2021 ◽  
Author(s):  
Yiyang Lin ◽  
Matthew Penna ◽  
Christopher D. Spicer ◽  
Stuart G. Higgins ◽  
Amy Gelmi ◽  
...  
Keyword(s):  
High Throughput ◽  
Microtiter Plates ◽  
Peptide Derivatization

High-Throughput System for Screening of Cephalosporin C High-Yield Strain by 48-Deep-Well Microtiter Plates

2013 ◽  
Vol 169 (5) ◽  
pp. 1683-1695 ◽  
Author(s):  
Jun Tan ◽  
Ju Chu ◽  
Yuyou Hao ◽  
Yuanxin Guo ◽  
Yingping Zhuang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Yield ◽  
Cephalosporin C ◽  
Yield Strain ◽  
Deep Well ◽  
Microtiter Plates

High-Throughput Investigation of Polymerization Kinetics by Online Monitoring of GPC and GC

2004 ◽  
Vol 25 (1) ◽  
pp. 237-242 ◽  
Author(s):  
Richard Hoogenboom ◽  
Martin W. M. Fijten ◽  
Caroline H. Abeln ◽  
Ulrich S. Schubert
Keyword(s):  
High Throughput ◽  
Online Monitoring ◽  
Polymerization Kinetics

scholarly journals A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Anna Gärtner ◽  
Anna Joëlle Ruff ◽  
Ulrich Schwaneberg
Keyword(s):  
Capillary Electrophoresis ◽  
High Throughput ◽  
High Throughput Screening ◽  
Product Formation ◽  
Resonance Spectroscopy ◽  
P450 Bm3 ◽  
Microtiter Plates ◽  
Main Challenge ◽  
Total Turnover ◽  
Screening Systems

Abstract The main challenge that prevents a broader application of directed enzyme evolution is the lack of high-throughput screening systems with universal product analytics. Most directed evolution campaigns employ screening systems based on colorimetric or fluorogenic surrogate substrates or universal quantification methods such as nuclear magnetic resonance spectroscopy or mass spectrometry, which have not been advanced to achieve a high-throughput. Capillary electrophoresis with a universal UV-based product detection is a promising analytical tool to quantify product formation. Usage of a multiplex system allows the simultaneous measurement with 96 capillaries. A 96-multiplexed capillary electrophoresis (MP-CE) enables a throughput that is comparable to traditional direct evolution campaigns employing 96-well microtiter plates. Here, we report for the first time the usage of a MP-CE system for directed P450 BM3 evolution towards increased product formation (oxidation of alpha-isophorone to 4-hydroxy-isophorone; highest reached total turnover number after evolution campaign: 7120 mol4-OH molP450−1). The MP-CE platform was 3.5-fold more efficient in identification of beneficial variants than the standard cofactor (NADPH) screening system.

A high-throughput system for two-hybrid screening based on growth curve analysis in microtiter plates

2003 ◽  
Vol 316 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Claudia Diaz-Camino ◽  
Eddy P. Risseeuw ◽  
Enwu Liu ◽  
William L. Crosby
Keyword(s):  
High Throughput ◽  
Growth Curve ◽  
Curve Analysis ◽  
Growth Curve Analysis ◽  
Microtiter Plates ◽  
Two Hybrid ◽  
Hybrid Screening

scholarly journals Fluorescence Polarization Assays for High Throughput Screening of G Protein-Coupled Receptors

2000 ◽  
Vol 5 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Peter Banks ◽  
Mylene Gosselin ◽  
Linda Prystay
Keyword(s):  
High Throughput ◽  
Fluorescence Polarization ◽  
High Throughput Screening ◽  
Rank Order ◽  
Drug Binding ◽  
G Protein Coupled Receptors ◽  
Microtiter Plates ◽  
High Throughput Drug Screening ◽  
G Protein Coupled ◽  
Speed Of Analysis

Fluorescence polarization assays in 384-well microtiter plates have been demonstrated. The performance is suitable for high throughput drug screening applications with respect to speed of analysis, displaceable signal, precision, and sensitivity to various reagents. Rank order of potency was maintained relative to ['251]-ligand filtration assays, and the effects of the highly colored compounds, tartrazine and Chicago Sky Blue, were insignificant on the polarization signal up to a concentration of 1 tiM. These attributes suggest that accurate assessment of drug binding can be obtained.

Development of a high-throughput assay for measuring lipase activity using natural triacylglycerols coated on microtiter plates

The Analyst ◽  
2013 ◽  
Vol 138 (18) ◽  
pp. 5230 ◽  
Author(s):  
Carole Serveau-Avesque ◽  
Robert Verger ◽  
Jorge A. Rodriguez ◽  
Abdelkarim Abousalham
Keyword(s):  
High Throughput ◽  
Lipase Activity ◽  
Microtiter Plates ◽  
High Throughput Assay

scholarly journals High-Throughput Screening Assay for Inhibitors of TonB-Dependent Iron Transport

2015 ◽  
Vol 21 (3) ◽  
pp. 316-322 ◽  
Author(s):  
Mathew Hanson ◽  
Lorne D. Jordan ◽  
Yan Shipelskiy ◽  
Salete M. Newton ◽  
Phillip E. Klebba
Keyword(s):  
Outer Membrane ◽  
High Throughput ◽  
High Throughput Screening ◽  
Iron Transport ◽  
Iron Acquisition ◽  
Metabolic Inhibitors ◽  
Screening Assay ◽  
Gram Negative ◽  
Microtiter Plates ◽  
High Throughput Screening Assay

The TonB-dependent Gram-negative bacterial outer membrane protein FepA actively transports the siderophore ferric enterobactin (FeEnt) into the periplasm. We developed a high-throughput screening (HTS) assay that observes FeEnt uptake through FepA in living Escherichia coli, by monitoring fluorescence quenching that occurs upon binding of FeEnt, and then unquenching as the bacteria deplete it from solution by transport. We optimized the labeling and spectroscopic methods to screen for inhibitors of TonB-dependent iron uptake through the outer membrane. The assay works like a molecular switch that is on in the presence of TonB activity and off in its absence. It functions in 96-well microtiter plates, in a variety of conditions, with Z factors of 0.8–1.0. TonB-dependent iron transport is energy dependent, and the inhibitory effects of the metabolic inhibitors carbonyl cyanide m-chlorophenylhydrazone, 2,4-dinitrophenol, azide, cyanide, and arsenate on FeEnt uptake were readily detected by the assay. Because iron acquisition is a determinant of bacterial pathogenesis, HTS with this method may identify inhibitors that block TonB function and constitute novel therapeutics against infectious disease caused by Gram-negative bacteria.

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