scholarly journals Optimal Conditional Screening in High-Throughput Bioprocess Development

2018 ◽  
Vol 90 (9) ◽  
pp. 1224-1224 ◽  
Author(s):  
S. Hans ◽  
E. Anane ◽  
F. Glauche ◽  
P. Neubauer ◽  
M. N. Cruz-Bournazou ◽  
...  
Keyword(s):  
High Throughput ◽  
Bioprocess Development ◽  
High Throughput Bioprocess Development

Automated disposable small scale reactor for high throughput bioprocess development: A proof of concept study

2013 ◽  
Vol 110 (12) ◽  
pp. 3126-3138 ◽  
Author(s):  
Rachel Bareither ◽  
Neil Bargh ◽  
Robert Oakeshott ◽  
Kathryn Watts ◽  
David Pollard
Keyword(s):  
High Throughput ◽  
Small Scale ◽  
Proof Of Concept ◽  
Bioprocess Development ◽  
Concept Study ◽  
Scale Reactor ◽  
High Throughput Bioprocess Development

High-Throughput Bioprocess Development

2016 ◽  
Vol 36 (7) ◽  
pp. 30-31
Author(s):  
Christopher Kistler ◽  
Jennifer Pollard ◽  
Lee Sin Ng ◽  
Mathieu Streefland
Keyword(s):  
High Throughput ◽  
Bioprocess Development ◽  
High Throughput Bioprocess Development

Identifying analytics for high throughput bioprocess development studies

2013 ◽  
Vol 110 (7) ◽  
pp. 1924-1935 ◽  
Author(s):  
Spyridon Konstantinidis ◽  
Simyee Kong ◽  
Nigel Titchener-Hooker
Keyword(s):  
High Throughput ◽  
Development Studies ◽  
Bioprocess Development ◽  
High Throughput Bioprocess Development

scholarly journals Optimal operation of parallel mini-bioreactors in bioprocess development using multi-stage MPC

2021 ◽  
Author(s):  
Niels Krausch ◽  
Jong Woo Kim ◽  
Sergio Lucia ◽  
Sebastian Gross ◽  
Tilman Barz ◽  
...  
Keyword(s):  
High Throughput ◽  
Growth Models ◽  
Optimal Operation ◽  
Scenario Tree ◽  
Process Conditions ◽  
Bioprocess Development ◽  
Early Screening ◽  
Feedback Information ◽  
Control Approach ◽  
Multi Stage

Bioprocess development is commonly characterized by long development times, especially in the early screening phase. After promising candidates have been pre-selected in screening campaigns, an optimal operating strategy has to be found and verified under conditions similar to production. Cultivating cells with pulse-based feeding and thus exposing them to oscillating feast and famine phases has shown to be a powerful approach to study microorganisms closer to industrial bioreactor conditions. In view of the large number of strains and the process conditions to be tested, high-throughput cultivation systems provide an essential tool to sample the large design space in short time. We have recently presented a comprehensive platform, consisting of two liquid handling stations coupled with a model-based experimental design and operation framework to increase the efficiency in High Throughput bioprocess development. Using calibrated macro-kinetic growth models, the platform has been successfully used for the development of scale-down fed-batch cultivations in parallel mini-bioreactor systems. However, it has also been shown that parametric uncertainties in the models can significantly affect the prediction accuracy and thus the reliability of optimized cultivation strategies. To tackle this issue, we implemented a multi-stage Model Predictive Control (MPC) strategy to fulfill the experimental objectives under tight constraints despite the uncertainty in the parameters and the measurements. Dealing with uncertainties in the parameters is of major importance, since constraint violation would easily occur otherwise, which in turn could have adverse effects on the quality of the heterologous protein produced. Multi-stage approaches build up scenario tree, based on the uncertainty that can be encountered and computing optimal inputs that satisfy the constrains despite of such uncertainties. Using the feedback information gained through the evolution along the tree, the control approach is significantly more robust than standard MPC approaches without being overly conservative. We show in this study that the application of multi-stage MPC can increase the number of successful experiments, by applying this methodology to a mini-bioreactor cultivation operated in parallel.

The development and application of high throughput cultivation technology in bioprocess development

2014 ◽  
Vol 192 ◽  
pp. 323-338 ◽  
Author(s):  
Quan Long ◽  
Xiuxia Liu ◽  
Yankun Yang ◽  
Lu Li ◽  
Linda Harvey ◽  
...  
Keyword(s):  
High Throughput ◽  
Bioprocess Development ◽  
Cultivation Technology

High-Throughput Technologies in Bioprocess Development

2010 ◽  
Author(s):  
Trent Carrier ◽  
Eva Heldin ◽  
Mattias Ahnfelt ◽  
Eggert Brekkan ◽  
Richard Hassett ◽  
...  
Keyword(s):  
High Throughput ◽  
Bioprocess Development

A microfluidic approach to high throughput quantification of host cell protein impurities for bioprocess development

2014 ◽  
Vol 2 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Jun Hyuk Heo ◽  
Xiaodun Mou ◽  
Fengqiang Wang ◽  
John M Troisi ◽  
Christopher W Sandifer ◽  
...  
Keyword(s):  
Host Cell ◽  
High Throughput ◽  
Cell Protein ◽  
Bioprocess Development ◽  
Host Cell Protein
Sign in / Sign up

Export Citation Format

Share Document