Development of a New Smartphone Photometer for Enzymatic Bioassays

2016 ◽  
Vol 88 (9) ◽  
pp. 1307-1308
Author(s):  
M. Schäfer ◽  
R. Ulber
Keyword(s):  
Enzymatic Bioassays

Highly sensitive colorimetric detection of glucose and uric acid in biological fluids using chitosan-modified paper microfluidic devices

The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4749-4756 ◽  
Author(s):  
Ellen F. M. Gabriel ◽  
Paulo T. Garcia ◽  
Thiago M. G. Cardoso ◽  
Flavio M. Lopes ◽  
Felipe T. Martins ◽  
...  
Keyword(s):  
Uric Acid ◽  
Biological Fluids ◽  
Colorimetric Detection ◽  
Microfluidic Devices ◽  
Analytical Performance ◽  
Highly Sensitive ◽  
Colorimetric Measurements ◽  
Paper Microfluidic ◽  
Enzymatic Bioassays

This paper describes the modification of microfluidic paper-based analytical devices (μPADs) with chitosan to improve the analytical performance of colorimetric measurements associated with enzymatic bioassays.

scholarly journals Set of Enzymatic Bioassays for Assessment of Soil Contamination

2019 ◽  
Vol 489 (1) ◽  
pp. 165-168
Author(s):  
E. M. Kolosova ◽  
O. S. Sutormin ◽  
E. N. Esimbekova ◽  
V. I. Lonshakova-Mukina ◽  
V. A. Kratasyuk
Keyword(s):  
Soil Contamination ◽  
Enzymatic Bioassays

scholarly journals Behavioral and enzymatic bioassays with Serjania erecta Radlk., Sapindaceae, correlated with cognitive dysfunctions

2010 ◽  
Vol 20 (4) ◽  
pp. 519-528 ◽  
Author(s):  
Luciana S. C. Broggini ◽  
Renata S. Fernandes ◽  
Thiago Nogueira ◽  
Fernanda R. Suzano ◽  
Ariadiny L. Caetano ◽  
...  
Keyword(s):  
Cognitive Dysfunctions ◽  
Enzymatic Bioassays

scholarly journals Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1499
Author(s):  
Lyubov S. Bondarenko ◽  
Ekaterina S. Kovel ◽  
Kamila A. Kydralieva ◽  
Gulzhian I. Dzhardimalieva ◽  
Erzsébet Illés ◽  
...  
Keyword(s):  
Magnetite Nanoparticles ◽  
Biological Effects ◽  
Photobacterium Phosphoreum ◽  
Hydrodynamic Diameter ◽  
Enzymatic Reactions ◽  
Bacterial Cells ◽  
Low Concentration ◽  
Negative Surface ◽  
Negative Surface Charge ◽  
Enzymatic Bioassays

Current paper presents biological effects of magnetite nanoparticles (MNPs). Relations of MNP’ characteristics (zeta-potential and hydrodynamic diameters) with effects on bacteria and their enzymatic reactions were the main focus. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc.

scholarly journals Set of enzymatic bioassays for assessment of soil pollution

2019 ◽  
Vol 489 (1) ◽  
pp. 103-107
Author(s):  
Е. М. Kolosova ◽  
О. S. Sutormin ◽  
Е. N. Esimbekova ◽  
V. I. Lonshakova-Mukina ◽  
V. А. Kratasyuk
Keyword(s):  
Enzymatic Reactions ◽  
Toxic Substances ◽  
Test Functions ◽  
Luminous Bacteria ◽  
Enzymatic Bioassays ◽  
Living Organisms ◽  
The Impact ◽  
Potential Test ◽  
Test Objects ◽  
Live Organism

A concept of the comprehensive assessment of soil contamination is proposed. In this concept, the conclusion regarding the presence of toxic substances in a sample, which is being analyzed, is connected with the inhibition of enzymatic reactions that are responsible for various functions of a live organism, such as luminescence, respiration, etc., these functions are taken as test functions in classical bioassays, which are using live objects (luminous bacteria, daphnia, algae, etc.). The regularities of the impact of different classes of toxicants on the activity of individual enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects - markers of pollution. The set of enzymatic bioassays consists of three enzyme systems with maximum sensitivity to different classes of toxicants: butyrylcholinesterase, NAD(P)H:FMN‑oxidoreductase + luciferase and lactate dehydrogenase + NAD(P)H:FMN‑oxidoreductase + luciferase. The possibility of using enzymes instead of living organisms in the bioassay of natural complex systems is shown.

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