scholarly journals Anti‐MJ/NXP‐2 antibody‐positive adult‐onset dermatomyositis with lichen myxedematosus and endometrial carcinoma

2021 ◽  
Author(s):  
Kimiko Nakajima ◽  
Mayuko Yamamato ◽  
Risa Ohsawa ◽  
Hideki Nakajima ◽  
Nagamasa Maeda ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Adult Onset ◽  
Lichen Myxedematosus

Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells

1990 ◽  
Vol 48 (3) ◽  
pp. 944-945
Author(s):  
Ichiro Yamamoto ◽  
Toshiaki Tachibana ◽  
Hiroko Maruyama ◽  
Noriyuki Komatsu ◽  
Hiroyuki Kuramoto ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Cell Lines ◽  
Endometrial Adenocarcinoma ◽  
Protein A ◽  
Rabbit Antiserum ◽  
Carcinoma Cells ◽  
Affinity Column ◽  
Antibody Technique ◽  
Galactosyl Transferase ◽  
C Terminus

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation.An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO2. The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.

Lichen myxedematosus associated with chronic hepatitis C

2000 ◽  
Vol 39 (3) ◽  
pp. 212-214 ◽  
Author(s):  
Hiroyuki Banno ◽  
Hiromichi Takama ◽  
Yukiko Nitta ◽  
Toshihiko Ikeya ◽  
Yoshifumi Hirooka
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Lichen Myxedematosus

Adult-Onset Asthma Is Common But Largely Underrecognized

Ob Gyn News ◽  
2005 ◽  
Vol 40 (8) ◽  
pp. 46
Author(s):  
KATE JOHNSON
Keyword(s):  
Adult Onset

Large Study Links Rhinitis to Adult-Onset Asthma

2008 ◽  
Vol 38 (21) ◽  
pp. 30
Author(s):  
SHARON WORCESTER
Keyword(s):  
Large Study ◽  
Adult Onset
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