Chiral HPLC Separation and Modeling of Four Stereomers of DL-Leucine-DL-Tryptophan Dipeptide on Amylose Chiral Column

Chirality ◽  
2016 ◽  
Vol 28 (9) ◽  
pp. 642-648 ◽  
Author(s):  
Mohammed F. Alajmi ◽  
Afzal Hussain ◽  
Mohd. Suhail ◽  
Sofi Danish Mukhtar ◽  
Dibya Ranjan Sahoo ◽  
...  
Keyword(s):  
Chiral Hplc ◽  
Hplc Separation ◽  
Chiral Column

scholarly journals Simple and Rapid HPLC Separation and Quantification of Flavonoid, Flavonolignans, and 2,3-Dehydroflavonolignans in Silymarin

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 116 ◽  
Author(s):  
Petrásková ◽  
Káňová ◽  
Biedermann ◽  
Křen ◽  
Valentová
Keyword(s):  
Analytical Methods ◽  
Fourth Century ◽  
Preparative Chromatography ◽  
Hplc Separation ◽  
Silybum Marianum ◽  
Chiral Column ◽  
Disease Treatment ◽  
First Time ◽  
Analytical Separation

Herbal preparations from Silybum marianum have been used since the fourth century BC in liver disease treatment and against numerous other pathologies. Consumption of silymarin containing drugs and food supplements continues to increase. Precise, fast, reliable, and complex determination of all components of silymarin preparations is paramount for assessing its pharmacological quality. We present here simple and fast HPLC-DAD and LC-MS analytical methods for the determination and quantification of all known silymarin components, including 2,3-dehydroflavonolignans that has not been achieved so far. The first method, using a common C18 column, allows baseline separation of previously inseparable silychristin A, B, isosilychristin, and silydianin. Moreover, this method allowed detection of three so far unknown silymarin components. In addition, the first analytical separation of enantiomers of 2,3-dehydrosilybin was achieved using a Lux 3μ Cellulose-4 chiral column, providing even more accurate description of silymarin composition. 2,3-Dehydroflavonolignans were isolated for the first time from silymarin using preparative chromatography on C18 and ASAHIPAK columns, and 2,3-dehydrosilychristin and 2,3-dehydrosilybin were for the first time conclusively confirmed by HPLC, MS, and NMR to be silymarin components. Using the optimized analytical methods, six various silymarin preparations were analyzed showing substantial differences in the composition.

Effective control of optical purity by chiral HPLC separation for ester-based liquid crystalline materials forming anticlinic smectic phases

2020 ◽  
pp. 1-11
Author(s):  
Terézia Vojtylová-Jurkovičová ◽  
Petra Vaňkátová ◽  
Magdalena Urbańska ◽  
Věra Hamplová ◽  
David Sýkora ◽  
...  
Keyword(s):  
Liquid Crystalline ◽  
Optical Purity ◽  
Effective Control ◽  
Chiral Hplc ◽  
Hplc Separation ◽  
Crystalline Materials ◽  
Smectic Phases ◽  
Liquid Crystalline Materials

Chiral HPLC Separation on Derivatized Cyclofructan Versus Cyclodextrin Stationary Phases

2012 ◽  
Vol 45 (16) ◽  
pp. 2344-2358 ◽  
Author(s):  
Jiří Vozka ◽  
Květa Kalíková ◽  
Lucie Janečková ◽  
Daniel W. Armstrong ◽  
Eva Tesařová
Keyword(s):  
Stationary Phases ◽  
Chiral Hplc ◽  
Hplc Separation

The first chiral HPLC separation of dicarba-nido-undecarborate anions and their chromatographic behavior

Talanta ◽  
2021 ◽  
Vol 222 ◽  
pp. 121652
Author(s):  
Ondřej Horáček ◽  
Monika Papajová-Janetková ◽  
Bohumír Grüner ◽  
Lukáš Lochman ◽  
Petra Štěrbová-Kovaříková ◽  
...  
Keyword(s):  
Chromatographic Behavior ◽  
Chiral Hplc ◽  
Hplc Separation

scholarly journals Development and Validation of a Chiral Liquid Chromatographic Assay for Enantiomeric Separation and Quantification of Verapamil in Rat Plasma: Stereoselective Pharmacokinetic Application

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2091
Author(s):  
Mostafa S. Mohammed ◽  
Mohamed M. Hefnawy ◽  
Abdulrhman A. Al-Majed ◽  
Haitham K. Alrabiah ◽  
Nasser A. Algrain ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Internal Standard ◽  
Enantiomeric Separation ◽  
Rat Plasma ◽  
Core Shell ◽  
Chiral Hplc ◽  
Phase Extraction ◽  
Chiral Column ◽  
Time Curve

A novel, fast and sensitive enantioselective HPLC assay with a new core–shell isopropyl carbamate cyclofructan 6 (superficially porous particle, SPP) chiral column (LarihcShell-P, LSP) was developed and validated for the enantiomeric separation and quantification of verapamil (VER) in rat plasma. The polar organic mobile phase composed of acetonitrile/methanol/trifluoroacetic acid/triethylamine (98:2:0.05: 0.025, v/v/v/v) and a flow rate of 0.5 mL/min was applied. Fluorescence detection set at excitation/emission wavelengths 280/313 nm was used and the whole analysis process was within 3.5 min, which is 10-fold lower than the previous reported HPLC methods in the literature. Propranolol was selected as the internal standard. The S-(−)- and R-(+)-VER enantiomers with the IS were extracted from rat plasma by utilizing Waters Oasis HLB C18 solid phase extraction cartridges without interference from endogenous compounds. The developed assay was validated following the US-FDA guidelines over the concentration range of 1–450 ng/mL (r2 ≥ 0.997) for each enantiomer (plasma) and the lower limit of quantification was 1 ng/mL for both isomers. The intra- and inter-day precisions were not more than 11.6% and the recoveries of S-(−)- and R-(+)-VER at all quality control levels ranged from 92.3% to 98.2%. The developed approach was successfully applied to the stereoselective pharmacokinetic study of VER enantiomers after oral administration of 10 mg/kg racemic VER to Wistar rats. It was found that S-(−)-VER established higher Cmax and area under the concentration-time curve (AUC) values than the R-(+)-enantiomer. The newly developed approach is the first chiral HPLC for the enantiomeric separation and quantification of verapamil utilizing a core–shell isopropyl carbamate cyclofructan 6 chiral column in rat plasma within 3.5 min after solid phase extraction (SPE).

Preparation of (R)-(-)-6,6'-Bis(3,5-dinitrobenzamido)biphenyl-2,2'-dicarboxylic Acid, a Potential Chiral HPLC Selector

1995 ◽  
Vol 60 (12) ◽  
pp. 2161-2164 ◽  
Author(s):  
Miloš Tichý ◽  
Jiří Závada
Keyword(s):  
Stationary Phase ◽  
Dicarboxylic Acid ◽  
Chiral Stationary Phase ◽  
Optically Active ◽  
Chiral Hplc ◽  
Hplc Separation ◽  
Separation Of Enantiomers

The title optically active diacid I has been prepared as a potential selector for chiral stationary phase in the HPLC separation of enantiomers.

A novel chiral column for the HPLC separation of a series of dansyl amino and arylalkanoic acids

Talanta ◽  
2008 ◽  
Vol 76 (5) ◽  
pp. 1261-1264 ◽  
Author(s):  
Yves-Claude Guillaume ◽  
Claire André
Keyword(s):  
Hplc Separation ◽  
Chiral Column

Chiral HPLC separation and CD spectra of the enantiomers of a molecular ‘hamburger’

2004 ◽  
Vol 14 (6) ◽  
pp. 237-239 ◽  
Author(s):  
Salvatore Caccamese ◽  
Nunziatina Parrinello ◽  
Salvatore Failla ◽  
Giuseppe A. Consiglio ◽  
Paolo Finocchiaro
Keyword(s):  
Chiral Hplc ◽  
Cd Spectra ◽  
Hplc Separation
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