ChemInform Abstract: Synthetic Profile to Various Thiazoloquinolines

ChemInform ◽  
2016 ◽  
Vol 47 (9) ◽  
pp. no-no
Author(s):  
Bakr F. Abdel-Wahab
Keyword(s):  
Synthetic Profile

Evaluation of the technique of immunosurgery for the isolation of inner cell masses from mouse blastocysts

Development ◽  
1977 ◽  
Vol 37 (1) ◽  
pp. 217-226
Author(s):  
A. H. Handyside ◽  
S. C. Barton
Keyword(s):  
Rabbit Antiserum ◽  
Trophoblast Cell ◽  
Trophoblast Cells ◽  
Rabbit Igg ◽  
Developmental Capacity ◽  
Synthetic Profile

Inner cell masses (ICMs) immunosurgically-isolated from 3½-day mouse blastocysts were examined for trophoblast cell contamination and developmental capacity. Blastocysts were preincubated in rabbit anti-mouse antiserum, washed thoroughly and then incubated in complement. The ICMs were then easily dissected by drawing through a fine pipette. Various experiments confirmed that the trophectoderm had been completely removed by this treatment. Firstly, the ICMs did not bind a fiuorescein-conjugated antibody directed against rabbit IgG, indicating the absence of cells exposed to the rabbit antiserum during the immunosurgical procedure. Secondly, ICMs dissected from blastocysts preincubated in a suspension of melanin granules did not include any of the trophoblast cells that had phagocytosed the granules. And, thirdly, the protein synthetic profile of these ICMs was similar to microsurgically dissected ICMs, and in particular, trophoblast specific spots were absent. The developmental capacity of immunosurgically-isolated ICMs was tested by injecting them into blastocysts and transferring to the uterus of 2½-day pseudopregnant recipients. Extensive chimaerism was detected in the majority of implants, 5–6 days after transfer, but only in ICM-derived tissues. This demonstrates both the lack of trophoblast cell contamination and functional viability of these ICMs.

Peripheral blood lymphocytes of bipolar affective patients have a histone synthetic profile indicative of an active cell state

1998 ◽  
Vol 22 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Thomae G. Sourlingas ◽  
Marietta R. Issidorides ◽  
Sophia Havaki ◽  
George Trikkas ◽  
Kalliope E. Sekeri-Pataryas
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Active Cell ◽  
Blood Lymphocytes ◽  
Cell State ◽  
Synthetic Profile

Synthetic profile of thiadiazoloquinazolines

2017 ◽  
Vol 192 (11) ◽  
pp. 1165-1170 ◽  
Author(s):  
Bakr F. Abdel-Wahab ◽  
Abdelbasset A. Farahat ◽  
Mohamed S. Bekheit
Keyword(s):  
Synthetic Profile

Validation Test Report for the Improved Synthetic Ocean Profile (ISOP) System, Part I: Synthetic Profile Methods and Algorithm

2013 ◽  
Author(s):  
Robert W. Helber ◽  
Tamara L. Townsend ◽  
Charlie N. Barron ◽  
Jan M. Dastugue ◽  
Michael R. Carnes
Keyword(s):  
Test Report ◽  
Validation Test ◽  
System Part ◽  
Synthetic Profile

scholarly journals Expression of embryonic globins by erythroid cells in juvenile chronic myelocytic leukemia

Blood ◽  
1991 ◽  
Vol 77 (12) ◽  
pp. 2569-2576 ◽  
Author(s):  
T Papayannopoulou ◽  
B Nakamoto ◽  
NP Anagnou ◽  
D Chui ◽  
L Dow ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Fetal Hemoglobin ◽  
Colony Growth ◽  
Leukemic Cells ◽  
Chronic Myelocytic Leukemia ◽  
Erythroid Cells ◽  
Myelocytic Leukemia ◽  
Synthetic Profile

Abstract Juvenile chronic myelocytic leukemia (JCML) is a rare hematopoietic neoplasia of early childhood with distinct hematologic and biochemical features. We studied the biologic properties and the globin synthetic profiles of JCML erythroid cells both in vivo and in vitro from a total of 24 patients. In these cases we observed the exuberant colony-forming unit-macrophage (CFU-M) colony growth, as reported previously. Furthermore, in contrast to previous reports, we found significant erythroid colony growth in most of our cases (average: 1,182 burst- forming unit-erythroid [BFUe] per 10(5) plated cells, range: 40 to 6,927). This growth was by and large erythropoietin-dependent and was not greatly influenced by other added cytokines. By several criteria all erythroid colony growth detected in vitro was derived from JCML progenitors. The globin synthetic profile of JCML erythroid cells showed high levels of fetal hemoglobin both in vivo and in vitro (gamma/gamma + beta: 53% to 94% in reticulocytes, 62% to 98% in BFUe- derived cells). In addition (in seven cases studied) we detected embryonic globins (epsilon and zeta) at the protein and messenger RNA level, a novel finding for primary leukemic cells. We speculate that the transformed erythroid cells in JCML harbor a trans environment supporting expression of developmentally earlier genes (fetal, embryonic). However, in contrast to other acute or subacute leukemias, JCML erythroid cells also have the ability to reach full maturation to the red cell level, thus allowing detection of this primitive program in vivo.

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