The stability factors for Cr6+ and Cr3+ in microbiological media, including temperature, preservation conditions and medium components were studied in this research project, through potassium permanganate oxidation and DPC (Diphenylcarbazide) spectrophotometry. It shows that the protein component mainly influences Cr6+ content changes at pre- and post- heat sterilization to the chromium ionic liquid medium, other than being impacted basically by inorganic salt ions. It also indicates that the method can be introduced into experiment researches for microbe dechromisation i.e. Chromium ion aqueous solution and fluid medium are sterilized separately, and then are made into the chrome ions liquid as per a certain concentration. The concentration of hexavalent chromium ions is affected by preservation time and temperature also. For this reason, chromium ionic liquid medium is kept at low temperature, and as quickly as possible for the test.