Thermochemistry of Organic and Heteroorganic Species. Part 19. Structural Aspects and Thermochemical Approach to Isomerization and Fragmentation of Negative Ions: Skeletal Rearrangements, Molecular Ion and Artifacts

ChemInform ◽  
2006 ◽  
Vol 37 (25) ◽  
Author(s):  
Dmitry Ponomarev ◽  
Viatcheslav Takhistov
Keyword(s):  
Negative Ions ◽  
Molecular Ion ◽  
Skeletal Rearrangements ◽  
Structural Aspects

scholarly journals High-speed solar wind streams and polar mesosphere winter echoes at Troll, Antarctica

2015 ◽  
Vol 33 (6) ◽  
pp. 609-622 ◽  
Author(s):  
S. Kirkwood ◽  
A. Osepian ◽  
E. Belova ◽  
Y.-S. Lee
Keyword(s):  
Solar Wind ◽  
Electron Density ◽  
High Speed ◽  
Negative Ions ◽  
Solar Proton ◽  
Ion Chemistry ◽  
Molecular Ion ◽  
Speed Solar Wind ◽  
Solar Proton Events ◽  
High Speed Solar Wind

Abstract. A small, 54 MHz wind-profiler radar, MARA, was operated at Troll, Antarctica (72° S, 2.5° E), continuously from November 2011 to January 2014, covering two complete Antarctic winters. Despite very low power, MARA observed echoes from heights of 55–80 km (polar mesosphere winter echoes, PMWE) on 60% of all winter days (from March to October). This contrasts with previous reports from radars at high northern latitudes, where PWME have been reported only by very high power radars or during rare periods of unusually high electron density at PMWE heights, such as during solar proton events. Analysis shows that PWME at Troll were not related to solar proton events but were often closely related to the arrival of high-speed solar wind streams (HSS) at the Earth, with PWME appearing at heights as low as 56 km and persisting for up to 15 days following HSS arrival. This demonstrates that HSS effects penetrate directly to below 60 km height in the polar atmosphere. Using local observations of cosmic-noise absorption (CNA), a theoretical ionization/ion-chemistry model and a statistical model of precipitating energetic electrons associated with HSS, the electron density conditions during the HSS events are estimated. We find that PMWE detectability cannot be explained by these variations in electron density and molecular-ion chemistry alone. PWME become detectable at different thresholds depending on solar illumination and height. In darkness, PWME are detected only when the modelled electron density is above a threshold of about 1000 cm−3, and only above 75 km height, where negative ions are few. In daylight, the electron density threshold falls by at least 2 orders of magnitude and PWME are found primarily below 75 km height, even in conditions when a large proportion of negative ions is expected. There is also a strong dawn–dusk asymmetry with PWME detected very rarely during morning twilight but often during evening twilight. This behaviour cannot be explained if PMWE are caused by small-scale structure in the neutral/molecular-ion gas alone but may be explained by the presence of charged meteoric dust.

Structural aspects of osmoregulation in porphyra

1978 ◽  
Vol 36 (2) ◽  
pp. 412-413
Author(s):  
C. Wiencke ◽  
A. Lauchli
Keyword(s):  
Culture Medium ◽  
Point Of View ◽  
Chemical Fixation ◽  
Cellular Organization ◽  
Osmotic Adaptation ◽  
Osmotic Balance ◽  
Activity Of Enzymes ◽  
Osmotic Agents ◽  
Vacuolar System ◽  
Structural Aspects

Osmoregulatory mechanisms in algae were investigated mainly from a physiological point of view (KAUSS 1977, HELLEBUST 1976). In Porphyra two osmotic agents, i. e. floridoside/isofloridoside (KAUSS 1968) and certain ions, such as K+ and Na+(EPPLEY et al. 1960) are considered for osmotic balance. Accumulations of ions (particularly Na+) in the cytoplasm during osmotic adaptation is improbable, because the activity of enzymes is generally inhibited by high ionic concentrations (FLOWERS et al. 1977).The cellular organization of Porphyra was studied with special emphasis on the development of the vacuolar system under different hyperosmotic conditions. Porphyra was cultivated at various strengths of the culture medium ASP 12 (PROVASOLI 1961) ranging from normal to 6 times concentrated (6x) culture medium. Por electron microscopy freeze fracturing was used (specimens fixed in 2% glutaraldehyde and incubated in 30% glycerol, preparation in a BALZERS BA 360 M apparatus), because chemical fixation gave poor results.

Molecular Structure of the Outermost Cell Wall Component of Spirillum Serpens

1977 ◽  
Vol 35 ◽  
pp. 342-343
Author(s):  
Wah Chiu ◽  
David Grano
Keyword(s):  
Molecular Structure ◽  
Cell Wall ◽  
Outer Membrane ◽  
Gel Electrophoresis ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Cell Wall Component ◽  
Protein Components ◽  
Exposure Technique ◽  
Structural Aspects

The periodic structure external to the outer membrane of Spirillum serpens VHA has been isolated by similar procedures to those used by Buckmire and Murray (1). From SDS gel electrophoresis, we have found that the isolated fragments contain several protein components, and that the crystalline structure is composed of a glycoprotein component with a molecular weight of ∽ 140,000 daltons (2). Under an electron microscopic examination, we have visualized the hexagonally-packed glycoprotein subunits, as well as the bilayer profile of the outer membrane. In this paper, we will discuss some structural aspects of the crystalline glycoproteins, based on computer-reconstructed images of the external cell wall fragments.The specimens were prepared for electron microscopy in two ways: negatively stained with 1% PTA, and maintained in a frozen-hydrated state (3). The micrographs were taken with a JEM-100B electron microscope with a field emission gun. The minimum exposure technique was essential for imaging the frozen- hydrated specimens.

7-beam lattice images of (110) oriented Ge

1978 ◽  
Vol 36 (1) ◽  
pp. 290-291
Author(s):  
J.R. Parsons ◽  
C.W. Hoelke
Keyword(s):  
Image Features ◽  
Objective Lens ◽  
Lattice Plane ◽  
Lattice Image ◽  
Crystalline Defects ◽  
Transmission Electron ◽  
Lattice Images ◽  
Electron Microscopes ◽  
Structural Aspects ◽  
Beam Lattice

The direct imaging of a crystal lattice has intrigued electron microscopists for many years. What is of interest, of course, is the way in which defects perturb their atomic regularity. There are problems, however, when one wishes to relate aperiodic image features to structural aspects of crystalline defects. If the defect is inclined to the foil plane and if, as is the case with present 100 kV transmission electron microscopes, the objective lens is not perfect, then terminating fringes and fringe bending seen in the image cannot be related in a simple way to lattice plane geometry in the specimen (1).The purpose of the present work was to devise an experimental test which could be used to confirm, or not, the existence of a one-to-one correspondence between lattice image and specimen structure over the desired range of specimen spacings. Through a study of computed images the following test emerged.

Structural aspects of tracheary element differentiation in suspension cultures of Zinnia elegans

1989 ◽  
Vol 47 ◽  
pp. 768-769
Author(s):  
C. H. Haigler ◽  
A. W. Roberts
Keyword(s):  
Tracheary Element ◽  
Vesicle Fusion ◽  
Zinnia Elegans ◽  
Cellulose Synthesis ◽  
Tracheary Elements ◽  
Animal Cells ◽  
Mesophyll Cells ◽  
Fixation Techniques ◽  
Localized Patterns ◽  
Structural Aspects

Tracheary elements, the water-conducting cells in plants, are characterized by their reinforced walls that became thickened in localized patterns during differentiation (Fig. 1). The synthesis of this localized wall involves abundant secretion of Golgi vesicles that export preformed matrix polysaccharides and putative proteins involved in cellulose synthesis. Since the cells are not growing, some kind of endocytotic process must also occur. Many researchers have commented on where exocytosis occurs in relation to the thickenings (for example, see), but they based their interpretations on chemical fixation techniques that are not likely to provide reliable information about rapid processes such as vesicle fusion. We have used rapid freezing to more accurately assess patterns of vesicle fusion in tracheary elements. We have also determined the localization of calcium, which is known to regulate vesicle fusion in plant and animal cells.Mesophyll cells were obtained from immature first leaves of Zinnia elegans var. Envy (Park Seed Co., Greenwood, S.C.) and cultured as described previously with the following exceptions: (a) concentration of benzylaminopurine in the culture medium was reduced to 0.2 mg/l and myoinositol was eliminated; and (b) 1.75ml cultures were incubated in 22 x 90mm shell vials with 112rpm rotary shaking. Cells that were actively involved in differentiation were harvested and frozen in solidifying Freon as described previously. Fractures occurred preferentially at the cell/planchet interface, which allowed us to find some excellently-preserved cells in the replicas. Other differentiating cells were incubated for 20-30 min in 10(μM CTC (Sigma), an antibiotic that fluoresces in the presence of membrane-sequestered calcium. They were observed in an Olympus BH-2 microscope equipped for epi-fluorescence (violet filter package and additional Zeiss KP560 barrier filter to block chlorophyll autofluorescence).

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