Hydroamination: The Direct Addition of Amines to Alkenes and Alkynes

ChemInform ◽  
2005 ◽  
Vol 36 (28) ◽  
Author(s):  
Thomas E. Mueller
Keyword(s):  
Direct Addition

scholarly journals CLytA-DAAO Chimeric Enzyme Bound to Magnetic Nanoparticles. A New Therapeutical Approach for Cancer Patients?

2021 ◽  
Vol 22 (3) ◽  
pp. 1477
Author(s):  
María Fuentes-Baile ◽  
Elizabeth Pérez-Valenciano ◽  
Pilar García-Morales ◽  
Camino de Juan Romero ◽  
Daniel Bello-Gil ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Colorectal Carcinoma ◽  
Acid Oxidase ◽  
Oxygen Species ◽  
Cell Models ◽  
Amino Acid Oxidase ◽  
Direct Addition ◽  
Alginate Capsules ◽  
Anti Cancer ◽  
The Stability

D-amino acid oxidase (DAAO) is an enzyme that catalyzes the oxidation of D-amino acids generating H2O2. The enzymatic chimera formed by DAAO bound to the choline-binding domain of N-acetylmuramoyl-L-alanine amidase (CLytA) induces cytotoxicity in several pancreatic and colorectal carcinoma and glioblastoma cell models. In the current work, we determined whether the effect of CLytA-DAAO immobilized in magnetic nanoparticles, gold nanoparticles, and alginate capsules offered some advantages as compared to the free CLytA-DAAO. Results indicate that the immobilization of CLytA-DAAO in magnetic nanoparticles increases the stability of the enzyme, extending its time of action. Besides, we compared the effect induced by CLytA-DAAO with the direct addition of hydrogen peroxide, demonstrating that the progressive generation of reactive oxygen species by CLytA-DAAO is more effective in inducing cytotoxicity than the direct addition of H2O2. Furthermore, a pilot study has been initiated in biopsies obtained from pancreatic and colorectal carcinoma and glioblastoma patients to evaluate the expression of the main genes involved in resistance to CLytA-DAAO cytotoxicity. Based on our findings, we propose that CLytA-DAAO immobilized in magnetic nanoparticles could be effective in a high percentage of patients and, therefore, be used as an anti-cancer therapy for pancreatic and colorectal carcinoma and glioblastoma.

Properties of films from fish gelatin prepared by molecular modification and direct addition of oxidized linoleic acid

2019 ◽  
Vol 88 ◽  
pp. 291-300 ◽  
Author(s):  
Wipawee Theerawitayaart ◽  
Thummanoon Prodpran ◽  
Soottawat Benjakul ◽  
Pornsatit Sookchoo
Keyword(s):  
Linoleic Acid ◽  
Fish Gelatin ◽  
Molecular Modification ◽  
Direct Addition

scholarly journals Stereoselective Synthesis of α-Silylamines by the Direct Addition of Silyl Anions to Activated Imines.

ChemInform ◽  
2005 ◽  
Vol 36 (33) ◽  
Author(s):  
David M. Ballweg ◽  
Rebecca C. Miller ◽  
Danielle L. Gray ◽  
Karl A. Scheidt
Keyword(s):  
Stereoselective Synthesis ◽  
Direct Addition
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