Molecular Electron Affinities and the Calculation of the Temperature Dependence of the Electron-Capture Detector Response

ChemInform ◽  
2004 ◽  
Vol 35 (37) ◽  
Author(s):  
E. C. M. Chen ◽  
E. S. Chen
Keyword(s):  
Temperature Dependence ◽  
Electron Capture ◽  
Electron Capture Detector ◽  
Detector Response ◽  
Electron Affinities ◽  
Molecular Electron

Capillary Gas Chromatographic Determination with Electron Capture Detector of β-Propiolactone in Biological Materials

1991 ◽  
Vol 74 (4) ◽  
pp. 595-599
Author(s):  
Aziz Geahchan ◽  
Bruno Fouillet ◽  
Paul Chambon ◽  
Renee Chambon ◽  
Bahia Nouri
Keyword(s):  
Electron Capture ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Biological Materials ◽  
Electron Capture Detector ◽  
Detector Response ◽  
Limit Of Quantitation ◽  
Low Levels ◽  
Ppm Level

Abstract A method has been developed for the determination of β- propiolactone by derivatizing it to the volatile N-hexyl-3- heptafluorobutanoyloxypropanamide, which can be separated and Identified by a capillary CP-Sil 8 column, and detected by an electron capture detector (ECD). First, β- propiolactone is reacted with N-hexylamine to yield N-hexyl- 3-hydroxypropanamide. The fluorobutanoyl ester derivative is next prepared by using heptafluorobutyrlc acid anhydride in the presence of trimethylamine. The method Is very sensitive, simple, and specific, and can be used to detect and quantitate residual β-propiolactone In Iyophilized biological materials. The limit of detection is 0.2 ppm β-proplolactone in a 50 mg sample; however, because of variability at low levels, the limit of quantitation is 1 ppm. Detector response was linear for 2-500 ng β-propiolactone. Recoveries were 98% or greater from Iyophilized vaccines spiked at the 2-20 ppm level. No side products or interference peaks were observed in the derivatization reaction.

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