Synthesis of Pyrimidine Derivatives Possessing an Antioxidative Property and Their Inhibitory Effects on Picryl Chloride-Induced Contact Hypersensitivity Reaction.

ChemInform ◽  
2004 ◽  
Vol 35 (17) ◽  
Author(s):  
Yoshiaki Isobe ◽  
Kosaku Hirota
Keyword(s):  
Hypersensitivity Reaction ◽  
Contact Hypersensitivity ◽  
Inhibitory Effects ◽  
Pyrimidine Derivatives ◽  
Picryl Chloride ◽  
Antioxidative Property

Inhibitory Activities of Novel Pyrimidine Derivatives on the Contact Hypersensitivity Reaction.

ChemInform ◽  
2003 ◽  
Vol 34 (33) ◽  
Author(s):  
Yoshiaki Isobe ◽  
Masanori Tobe ◽  
Yoshifumi Inoue ◽  
Yuso Goto ◽  
Fumihiro Obara ◽  
...  
Keyword(s):  
Hypersensitivity Reaction ◽  
Contact Hypersensitivity ◽  
Pyrimidine Derivatives ◽  
Inhibitory Activities

scholarly journals Inhibitory Activities of Novel Pyrimidine Derivatives on the Contact Hypersensitivity Reaction

2003 ◽  
Vol 51 (3) ◽  
pp. 309-312 ◽  
Author(s):  
Yoshiaki Isobe ◽  
Masanori Tobe ◽  
Yoshifumi Inoue ◽  
Yuso Goto ◽  
Fumihiro Obara ◽  
...  
Keyword(s):  
Hypersensitivity Reaction ◽  
Contact Hypersensitivity ◽  
Pyrimidine Derivatives ◽  
Inhibitory Activities

scholarly journals SBF-1 inhibits contact hypersensitivity in mice through down-regulation of T-cell-mediated responses

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Wei Chen ◽  
Xianying Fang ◽  
Yuan Gao ◽  
Ke Shi ◽  
Lijun Sun ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Contact Hypersensitivity ◽  
Immunosuppressive Activity ◽  
Con A ◽  
Activated T Cells ◽  
Picryl Chloride

Abstract Background T lymphocytes play an important role in contact hypersensitivity. This study aims to explore the immunosuppressive activity of SBF-1, an analog of saponin OSW-1, against T lymphocytes in vitro and in vivo. Methods Proliferation of T lymphocytes from lymph nodes of mice was determined by MTT assay. Flow cytometry analysis was performed to assess T cell activation and apoptosis. Levels of cytokines were determined by PCR and ELISA. BALB/c mice were sensitized and challenged with picryl chloride and thickness of left and right ears were measured. Results SBF-1 effectively inhibited T lymphocytes proliferation induced by concanavalin A (Con A) or anti-CD3 plus anti-CD28 at a very low dose (10 nM) but exhibited little toxicity in non-activated T lymphocytes at concentrations up to 10 μM. In addition, SBF-1 inhibited the expression of CD25 and CD69, as well as he phosphorylation of AKT in Con A-activated T cells. SBF-1 also induced apoptosis of activated T cells. In addition, SBF-1 also downregulated the induction of the T cell cytokines, IL-2 and IFN-γ in a dose-dependent manner. Furthermore, SBF-1 significantly suppressed ear swelling and inflammation in a mouse model of picryl chloride-induced contact hypersensitivity. Conclusions Our findings suggest that SBF-1 has an unique immunosuppressive activity both in vitro and in vivo mainly through inhibiting T cell proliferation and activation. Its mechanism appears to be related to the blockage of AKT signaling pathway.

scholarly journals Low zone tolerance to contact allergens in mice: a functional role for CD8+ T helper type 2 cells.

1996 ◽  
Vol 183 (3) ◽  
pp. 759-768 ◽  
Author(s):  
K Steinbrink ◽  
C Sorg ◽  
E Macher
Keyword(s):  
T Cells ◽  
Normal Skin ◽  
Suppressor Cells ◽  
Immunological Tolerance ◽  
Contact Hypersensitivity ◽  
Suitable Model ◽  
Lymph Node Cells ◽  
Picryl Chloride ◽  
State Of Affairs

Normal skin is permeable to low molecular hydrophobic substances, including allergenic chemicals. Whereas such foreign matter appears to enter the skin naturally, it rarely induces contact hypersensitivity. This suggests that immunological tolerance would be the normal state of affairs. In search of a suitable model, we painted picryl chloride or oxazolone once or repeatedly on normal skin of BALB/c or C57B1/6 mice and found subsensitizing doses to be tolerogenic. The most effective doses in inducing tolerance were doses between those at the point of inflection from no responses to threshold sensitivity. But even doses three orders of magnitude lower than these suppressed subsequent sensitization if applied repeatedly. C57B1/6 mice (low responders) were consistently easier to make tolerant than BALB/c mice (high responders). The tolerant state established by a single painting was found to be fully developed at 48 h after initiation and long-lasting (>14 d). It could be adoptively transferred by intravenous injection of total spleen cells (SC), lymph node cells (LNC), or purified T cells and shown to be hapten specific. Pretreatment with cyclophosphamide (Cy) prevented tolerization. The T cells capable of transferring suppressive activity were found to be generated irrespective of the dose applied. On day 2 after painting, tolerance could be transferred with LNC from both tolerant and sensitized animals. On day 5, however, only cells from tolerant donors transferred tolerance. But by action of Cy, suppression was shown to be part of every sensitization, although masked. Production of hapten-specific antibodies was suppressed as well. Through depletion by monoclonal antibody in vitro the T suppressor cells were shown to belong to the murine CD8+ subset (Lyt2+). Upon restimulation in vitro by haptenized and irradiated normal SC, LNC from tolerant donors produced predominantly interleukin (IL)-4, IL-5, and IL-10. In contrast, LNC from sensitized donors produced preferentially IL-2 and interferon-gamma. Thus we demonstrate that painting subsensitizing doses of contact sensitizers on normal murine skin generates CD8+ Th2-like cells that give rise to hapten-specific tolerance. The model may have broader significance and apply to other species, including humans.

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