ChemInform Abstract: Synthesis and Photophysical Properties of New Fluorinated Benzo[c]xanthene Dyes as Intracellular pH Indicators.

ChemInform ◽  
2010 ◽  
Vol 33 (11) ◽  
pp. no-no
Author(s):  
Jixiang Liu ◽  
Zhenjun Diwu ◽  
Wai-Yee Leung
Keyword(s):  
Intracellular Ph ◽  
Photophysical Properties ◽  
Xanthene Dyes ◽  
Ph Indicators

Photophysical properties and interactions of xanthene dyes in aqueous micelles

2012 ◽  
Vol 247 ◽  
pp. 8-15 ◽  
Author(s):  
Diogo Silva Pellosi ◽  
Bianca Martins Estevão ◽  
Juliana Semensato ◽  
Divinomar Severino ◽  
Mauricio S. Baptista ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Xanthene Dyes ◽  
Aqueous Micelles

scholarly journals Xanthene-dye-labelled phosphatidylethanolamines as probes of interfacial pH. Studies in phospholipid vesicles

1989 ◽  
Vol 258 (3) ◽  
pp. 683-687 ◽  
Author(s):  
C G Knight ◽  
T Stephens
Keyword(s):  
Phospholipid Vesicles ◽  
Amino Group ◽  
Cell Surfaces ◽  
Vesicle Membrane ◽  
Xanthene Dyes ◽  
Ph Values ◽  
Ph Indicators ◽  
Membrane Bound ◽  
Single Emission ◽  
Hasselbalch Equation

We have been developing the use of plasma-membrane-bound fluorescent probes to measure the pH values at the surfaces of living chondrocytes. For this purpose, three lipophilic pH indicators were made by covalently binding the xanthene dyes fluorescein, eosin or dichlorofluorescein to the amino group of phosphatidylethanolamine. The probes were incorporated into phospholipid vesicles and the effect of pH on the fluorescence was characterized. Fluorescence was measured at a single emission wavelength during excitation at two wavelengths, and the ratio of the intensities was calculated. The experimentally observed pKobs. values were determined by fitting the fluorescence ratios to the Henderson-Hasselbalch equation. All three probes acted as pH indicators, and the eosinyl-, dichlorofluoresceinyl- and fluoresceinylphosphatidylethanolamines had pKobs. values of 3.5, 6.3 and 7.5 respectively. At physiological salt concentrations, changes in the composition of the vesicle membrane had little effect on these values. We concluded that these probes were promising candidates for the measurement of pH values at cell surfaces.

A novel method for absolute calibration of intracellular pH indicators

1989 ◽  
Vol 413 (5) ◽  
pp. 553-558 ◽  
Author(s):  
D. A. Eisner ◽  
N. A. Kenning ◽  
S. C. O'Neill ◽  
G. Pocock ◽  
C. D. Richards ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Absolute Calibration ◽  
Ph Indicators ◽  
Novel Method

MIGRATION OF THE ELECTRONIC EXCITATION ENERGY IN LANGMUIR – BLODGETT FILMS OF XANTHENE DYES

2001 ◽  
Vol 15 (28n30) ◽  
pp. 3948-3951 ◽  
Author(s):  
N. Kh. IBRAYEV ◽  
D. Zh. SATYABALDINA ◽  
A. M. ZHUNUSBEKOV
Keyword(s):  
Excitation Energy ◽  
Photophysical Properties ◽  
Delayed Fluorescence ◽  
Triplet States ◽  
Electronic Excitation Energy ◽  
Decay Kinetics ◽  
Xanthene Dyes ◽  
Lb Films ◽  
Langmuir Blodgett ◽  
Langmuir Blodgett Films

The concentration and temperature dependencies of the photophysical properties of three new amphiphilic xanthene dyes in the Langmuir-Blodgett (LB) films have been studied. The mechanisms of excitation-energy relaxation were discussed in terms of the excitons migration among energetically disordered monomers and the energy trapping by molecular aggregates. The experimental results were analyzed in view of carried out molecular mechanics and MO calcuations. Triplet states of eosin in the LB films were investigated. The observed nonexponential decay kinetics of delayed fluorescence (DF) and phosphorescence were analyzed in the framework of an annihilation model for both exchange and multipolar interactions.

Silicon-substituted Xanthene Dyes and Their Unique Photophysical Properties for Fluorescent Probes

2017 ◽  
Vol 12 (13) ◽  
pp. 1435-1446 ◽  
Author(s):  
Takayuki Ikeno ◽  
Tetsuo Nagano ◽  
Kenjiro Hanaoka
Keyword(s):  
Fluorescent Probes ◽  
Photophysical Properties ◽  
Xanthene Dyes

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

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