ChemInform Abstract: Asymmetric Reduction of Substituted Fluorenones with Aluminum Lithium Hydride in the Presence of Chiral Amino Alcohols.

ChemInform ◽  
2000 ◽  
Vol 31 (52) ◽  
pp. no-no
Author(s):  
Zhanru Yu ◽  
Francisco Lopez-Calahorra ◽  
Dolores Velasco
Keyword(s):  
Asymmetric Reduction ◽  
Amino Alcohols ◽  
Lithium Hydride ◽  
Aluminum Lithium ◽  
Chiral Amino Alcohols

ChemInform Abstract: Asymmetric Reduction of Ketones with Sodium Aluminum Hydride Modified with Chiral Amino Alcohols.

ChemInform ◽  
2010 ◽  
Vol 33 (1) ◽  
pp. no-no
Author(s):  
M. G. Vinogradov ◽  
O. V. Mikhalev ◽  
V. A. Pavlov ◽  
V. A. Ferapontov ◽  
O. R. Malyshev ◽  
...  
Keyword(s):  
Asymmetric Reduction ◽  
Aluminum Hydride ◽  
Amino Alcohols ◽  
Sodium Aluminum Hydride ◽  
Reduction Of Ketones ◽  
Chiral Amino Alcohols

scholarly journals Biosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in E. coli

2022 ◽  
Vol 9 ◽  
Author(s):  
Feifei Tong ◽  
Zongmin Qin ◽  
Hongyue Wang ◽  
Yingying Jiang ◽  
Junkuan Li ◽  
...  
Keyword(s):  
Reductive Amination ◽  
Asymmetric Reduction ◽  
Amino Alcohols ◽  
Saturation Mutagenesis ◽  
Preparative Scale ◽  
One Step ◽  
Amine Dehydrogenase ◽  
The One ◽  
Total Turnover ◽  
Chiral Amino Alcohols

Chiral amino alcohols are prevalent synthons in pharmaceuticals and synthetic bioactive compounds. The efficient synthesis of chiral amino alcohols using ammonia as the sole amino donor under mild conditions is highly desired and challenging in organic chemistry and biotechnology. Our previous work explored a panel of engineered amine dehydrogenases (AmDHs) derived from amino acid dehydrogenase (AADH), enabling the one-step synthesis of chiral amino alcohols via the asymmetric reductive amination of α-hydroxy ketones. Although the AmDH-directed asymmetric reduction is in a high stereoselective manner, the activity is yet fully excavated. Herein, an engineered AmDH derived from a leucine dehydrogenase from Sporosarcina psychrophila (SpAmDH) was recruited as the starting enzyme, and the combinatorial active-site saturation test/iterative saturation mutagenesis (CAST/ISM) strategy was applied to improve the activity. After three rounds of mutagenesis in an iterative fashion, the best variant wh84 was obtained and proved to be effective in the asymmetric reductive amination of 1-hydroxy-2-butanone with 4-fold improvements in kcat/Km and total turnover number (TTN) values compared to those of the starting enzyme, while maintaining high enantioselectivity (ee >99%) and thermostability (T5015 >53°C). In preparative-scale reaction, the conversion of 100 and 200 mM 1-hydroxy-2-butanone catalyzed by wh84 was up to 91–99%. Insights into the source of an enhanced activity were gained by the computational analysis. Our work expands the catalytic repertoire and toolbox of AmDHs.

Asymmetric reduction of ketoxime ethers to optically active O-substituted hydroxylamines with reagents prepared from borane and chiral amino alcohols

1997 ◽  
Vol 8 (4) ◽  
pp. 497-499 ◽  
Author(s):  
John T. Dougherty ◽  
Joseph R. Flisak ◽  
Jerome Hayes ◽  
Ivan Lantos ◽  
Li Liu ◽  
...  
Keyword(s):  
Asymmetric Reduction ◽  
Amino Alcohols ◽  
Optically Active ◽  
Chiral Amino Alcohols

Bile acid amides derived from chiral amino alcohols: novel antimicrobials and antifungals

2004 ◽  
Vol 14 (3) ◽  
pp. 773-777 ◽  
Author(s):  
BrajaG. Hazra ◽  
VandanaS. Pore ◽  
SanjeevKumar Dey ◽  
Suchitra Datta ◽  
MahendraP. Darokar ◽  
...  
Keyword(s):  
Bile Acid ◽  
Amino Alcohols ◽  
Chiral Amino Alcohols
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