ChemInform Abstract: Studies on Physiologically Active Substrates in Citrus Fruit Peel. Part 23. Isolation and Characterization of New Limonoid Glycosides (I) and (II) from Citrus unshiu Peels.

ChemInform ◽  
2010 ◽  
Vol 30 (36) ◽  
pp. no-no
Author(s):  
Akiyoshi Sawabe ◽  
Masanori Morita ◽  
Tatsuya Kiso ◽  
Hideki Kishine ◽  
Yoshikazu Ohtsubo ◽  
...  
Keyword(s):  
Citrus Fruit ◽  
Citrus Unshiu ◽  
Fruit Peel ◽  
Isolation And Characterization

Isolation and characterization of germacrene A synthases gene in Citrus unshiu Marc

2012 ◽  
Vol 145 ◽  
pp. 102-108 ◽  
Author(s):  
Takehiko Shimada ◽  
Tomoko Endo ◽  
Ana Rodríguez ◽  
Hiroshi Fujii ◽  
Michiharu Nakano ◽  
...  
Keyword(s):  
Citrus Unshiu ◽  
Isolation And Characterization

Isolation and characterization of new limonoid glycosides from Citrus unshiu peels

1999 ◽  
Vol 315 (1-2) ◽  
pp. 142-147 ◽  
Author(s):  
Akiyoshi Sawabe ◽  
Masanori Morita ◽  
Tatsuya Kiso ◽  
Hideki Kishine ◽  
Yoshikazu Ohtsubo ◽  
...  
Keyword(s):  
Citrus Unshiu ◽  
Isolation And Characterization

Isolation and characterization of (E)-beta-ocimene and 1,8 cineole synthases in Citrus unshiu Marc

Plant Science ◽  
2005 ◽  
Vol 168 (4) ◽  
pp. 987-995 ◽  
Author(s):  
Takehiko Shimada ◽  
Tomoko Endo ◽  
Hiroshi Fujii ◽  
Masakazu Hara ◽  
Mitsuo Omura
Keyword(s):  
Citrus Unshiu ◽  
Isolation And Characterization

scholarly journals Characterization of a caffeoyl-CoA O-methyltransferase-like enzyme involved in biosynthesis of polymethoxylated flavones in Citrus reticulata

2020 ◽  
Vol 71 (10) ◽  
pp. 3066-3079 ◽  
Author(s):  
Xiaojuan Liu ◽  
Chenning Zhao ◽  
Qin Gong ◽  
Yue Wang ◽  
Jinping Cao ◽  
...  
Keyword(s):  
Citrus Fruit ◽  
Citrus Reticulata ◽  
Fruit Peel ◽  
Polymethoxylated Flavones ◽  
Transient Overexpression ◽  
Highly Correlated

Abstract Polymethoxylated flavones (PMFs), which accumulate exclusively in fruit peel of citrus, play important physiological and pharmacological roles but the genetic basis for the methylation of flavonoids has not been fully elucidated in citrus. Here we characterize a caffeoyl-CoA O-methyltransferase-like enzyme, designated CrOMT1. The expression pattern of CrOMT1 was highly correlated with the concentration of the three major PMFs in two different citrus fruit tissues during fruit maturation. Exposure of fruit to UV-B radiation sharply increased the level of CrOMT1 transcripts and also led to the accumulation of three PMFs. The potential role of CrOMT1 was studied by testing the catalytic activity of recombinant CrOMT1 with numerous possible substrates in vitro. The enzyme could most efficiently methylate flavones with neighboring hydroxy moieties, with high catalytic efficiencies found with 6-OH- and 8-OH-containing compounds, preferences that correspond precisely with the essential methylation sites involved in the synthesis of the three naturally occurring PMFs in Citrus reticulata. This indicates that CrOMT1 is capable of in vitro methylation reactions required to synthesize PMFs in vivo. Furthermore, transient overexpression of CrOMT1 increased levels of the three major PMFs in fruit, indicating that CrOMT1 is likely to play an essential role in the biosynthesis of PMFs in citrus.

Isolation and characterization of the somatic embryogenesis receptor-like kinase gene homologue (CitSERK1) from Citrus unshiu Marc.

2005 ◽  
Vol 103 (2) ◽  
pp. 233-238 ◽  
Author(s):  
Takehiko Shimada ◽  
Toshiro Hirabayashi ◽  
Tomoko Endo ◽  
Hiroshi Fujii ◽  
Masayuki Kita ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Citrus Unshiu ◽  
Kinase Gene ◽  
Isolation And Characterization ◽  
Receptor Like Kinase ◽  
Gene Homologue

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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