ChemInform Abstract: Acetals and Ethers. Part 22. An Efficient Method for the Preparation of Pure Long-Chain cis- and trans-2-n-Alkyl-5-hydroxy-1,3-dioxanes.

ChemInform ◽  
2010 ◽  
Vol 28 (22) ◽  
pp. no-no
Author(s):  
A. PIASECKI ◽  
B. BURCZYK ◽  
A. SOKOLOWSKI ◽  
U. KOTLEWSKA
1996 ◽  
Vol 26 (22) ◽  
pp. 4145-4151 ◽  
Author(s):  
Andrzej Piasecki ◽  
Bogdan Burczyk ◽  
Adam Sokolowski ◽  
Urszula Kotlewska

2002 ◽  
Vol 301 (2) ◽  
pp. 168-174 ◽  
Author(s):  
Andrej-Nikolai Spiess ◽  
Richard Ivell

2002 ◽  
Vol 30 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
L. V. Michaelson ◽  
A. J. Longman ◽  
O. Sayanova ◽  
A. K. Stobart ◽  
J. A. Napier

We have isolated a cDNA encoding the Δ8 sphingolipid desaturase from the plant Aquilegia vulgaris L. via a PCR-based strategy using primers designed to target the conserved histidine box regions of microsomal desaturases. The function of the cDNA was confirmed by expression in the yeast, Saccharomyces cerevisiae. Analysis of the long-chain sphingoid bases as their dinitrophenyl derivatives by reverse-phase HPLC demonstrated the accumulation of cis- and trans-desaturated sphingoid bases which were not present in the wild-type yeast cells. The Δ8 desaturated products co-eluted with known Δ8-desaturated phytosphingenine and the molecular mass of these products was confirmed by liquid chromatography-MS. The Δ8 long-chain base desaturase was also able to desaturate dihydrosphingosine substrates. This is the first report of the functional characterization of an A. vulgaris gene product.


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