ChemInform Abstract: In situ Neutralization in Boc-Chemistry - Solid Phase Peptide Synthesis.

ChemInform ◽  
2010 ◽  
Vol 24 (7) ◽  
pp. no-no
Author(s):  
M. SCHNOELZER ◽  
P. ALEWOOD ◽  
A. JONES ◽  
D. ALEWOOD ◽  
S. B. H. KENT
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis

scholarly journals Robust Magnetized Graphene Oxide Platform for In Situ Peptide Synthesis and FRET-Based Protease Detection

Sensors ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 5275
Author(s):  
Seongsoo Kim ◽  
Sang-Myung Lee ◽  
Je Pil Yoon ◽  
Namhun Lee ◽  
Jinhyo Chung ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Resonance Energy ◽  
Precursor Solution ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Biomarker Analysis

Graphene oxide (GO)/peptide complexes as a promising disease biomarker analysis platform have been used to detect proteolytic activity by observing the turn-on signal of the quenched fluorescence upon the release of peptide fragments. However, the purification steps are often cumbersome during surface modification of nano-/micro-sized GO. In addition, it is still challenging to incorporate the specific peptides into GO with proper orientation using conventional immobilization methods based on pre-synthesized peptides. Here, we demonstrate a robust magnetic GO (MGO) fluorescence resonance energy transfer (FRET) platform based on in situ sequence-specific peptide synthesis of MGO. The magnetization of GO was achieved by co-precipitation of an iron precursor solution. Magnetic purification/isolation enabled efficient incorporation of amino-polyethylene glycol spacers and subsequent solid-phase peptide synthesis of MGO to ensure the oriented immobilization of the peptide, which was evaluated by mass spectrometry after photocleavage. The FRET peptide MGO responded to proteases such as trypsin, thrombin, and β-secretase in a concentration-dependent manner. Particularly, β-secretase, as an important Alzheimer’s disease marker, was assayed down to 0.125 ng/mL. Overall, the MGO platform is applicable to the detection of other proteases by using various peptide substrates, with a potential to be used in an automated synthesis system operating in a high throughput configuration.

Evaluation of COMU as a coupling reagent for in situ neutralization Boc solid phase peptide synthesis

2012 ◽  
Vol 18 (3) ◽  
pp. 199-207 ◽  
Author(s):  
Claudia U. Hjørringgaard ◽  
Andreas Brust ◽  
Paul F. Alewood
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Coupling Reagent

In situ neutralization in Boc-chemistry solid phase peptide synthesis

2009 ◽  
Vol 40 (3-4) ◽  
pp. 180-193 ◽  
Author(s):  
MARTINA SCHNölzer ◽  
PAUL ALEWOOD ◽  
ALUN JONES ◽  
DIANNE ALEWOOD ◽  
STEPHEN B.H. KENT
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis

In Situ Neutralization in Boc-chemistry Solid Phase Peptide Synthesis

2007 ◽  
Vol 13 (1-2) ◽  
pp. 31-44 ◽  
Author(s):  
Martina Schnölzer ◽  
Paul Alewood ◽  
Alun Jones ◽  
Dianne Alewood ◽  
Stephen B. H. Kent
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis

A Strategy for Thioamide Incorporation During Fmoc Solid-Phase Peptide Synthesis with Robust Stereochemical Integrity

2019 ◽  
Author(s):  
luis camacho III ◽  
Bryan J. Lampkin ◽  
Brett VanVeller
Keyword(s):  
Amino Acid ◽  
Functional Groups ◽  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Side Chains ◽  
Amino Acid Side Chains ◽  
Peptide Elongation

We describe a method to protect the sensitive stereochemistry of the thioamide—in analogy to the protection of the functional groups of amino acid side chains—in order to preserve the thioamide moiety during peptide elongation.<br>

Analytical Methods for Solid Phase Peptide Synthesis

2004 ◽  
Vol 8 (4) ◽  
pp. 291-301 ◽  
Author(s):  
Giuseppina Sabatino ◽  
Mario Chelli ◽  
Alberto Brandi ◽  
Anna Papini
Keyword(s):  
Peptide Synthesis ◽  
Analytical Methods ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis
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