ChemInform Abstract: Synthesis of Casein-Related Peptides and Phosphopeptides. Part 10. A Modified Method for the Synthesis of Ser(P)-Containing Peptides Through 4-Bromobenzyl Phosphate Protection.

ChemInform ◽  
2010 ◽  
Vol 22 (25) ◽  
pp. no-no
Author(s):  
J. W. PERICH ◽  
R. B. JOHNS
Keyword(s):  
Modified Method ◽  
Phosphate Protection

Synthesis of Casein-Related Peptides and Phosphopeptides. X. A Modified Method for the Synthesis of Ser(P)-Containing Peptides Through 4-Bromobenzyl Phosphate Protection

1991 ◽  
Vol 44 (3) ◽  
pp. 389 ◽  
Author(s):  
JW Perich ◽  
RB Johns
Keyword(s):  
Acetic Acid ◽  
Formic Acid ◽  
Peptide Synthesis ◽  
Acid Solutions ◽  
Isobutyl Alcohol ◽  
Modified Method ◽  
Step Procedure ◽  
One Step ◽  
Phosphate Protection

The three 4-halobenzyl phosphoramidite reagents di (4-fluorobenzyl) N,N- diisopropylphosphor-amidite, di (4-chlorobenzyl) N,N- diisopropylphosphoramidite and di (4-bromobenzyl) N,N-diethylphosphoramidite were prepared and used for the efficient phosphite-triester phosphorylation of isobutyl alcohol. While all three 4-halobenzyl groups were cleaved at similar rates from the 4-halobenzyl phosphorotriesters by 4 M HCl/dioxan or 50% CF3CO2H/CH2Cl2, the 4-bromobenzyl group had greater stability than either the 4-fluorobenzyl or 4-chlorobenzyl groups in formic acid or 1 M HCl /acetic acid solutions. The protected 4-bromobenzyl derivative, Boc-Ser(PO3BrBzl2)-OH, was prepared by a novel one-step procedure which featured di-4-bromobenzyl N,N- diethylphosphoramidite as phosphitylating agent. This derivative was used for the synthesis of Boc-Glu ( OBzl )-Ser(PO3BrBzl2)- Leu-OBzl by the Boc mode of peptide synthesis with 98% formic acid being used for the cleavage of the Boc group. Palladium-catalysed hydrogenolysis of the protected Ser(PO3BrBzl2) tripeptide in formic acid gave zwitterionic Glu -Ser(P)-Leu in high overall yield.

Proposal for Objective Evaluation of the Performance of Various Functional APC-resistance Tests in Genotyped Patients

1997 ◽  
Vol 78 (05) ◽  
pp. 1360-1365 ◽  
Author(s):  
G Freyburger ◽  
S Javorschi ◽  
S Labrouche ◽  
P Bernard
Keyword(s):  
Protein S ◽  
Objective Evaluation ◽  
Original Method ◽  
Screening Methods ◽  
Factor Xii ◽  
Factor V ◽  
Likelihood Ratios ◽  
Modified Method ◽  
Apc Resistance ◽  
Two Parameters

SummaryThe aim of the present study was to evaluate the relative performance of five screening methods for APC resistance caused by the factor V:Q506 mutation: the original method Coatest® APC™ Resistance Chromogenix, a modified method using the same reagents but a predilution 1+4 of the plasma in a factor V deficient plasma from Stago (Stago deficient V) or from Chromogenix (V-DEF Plasma), the Coatest® APC™ Resistance V (Chromogenix), and Accelerimat™ from bioMérieux. Normalization was done against a pool of normal plasmas for the methods from Chromogenix. The study included 350 subjects, 219 were genotyped (174 FV:R506R, 42 FV:Q506R, 3 FV:Q506Q) and most of them were assessed by more than one method. Uncertainty in predicting the FV genotype was evaluated by statistical analysis, which provided a way to quantitate the performance of the different diagnostic approaches. Performance of each test was evaluated by its sensitivity, specificity, R.O.C. curves, positive and negative likelihood ratios (LR), and the overall performance was determined by two parameters derived from the LR curves : the maximum LR value obtained at the crossover of the two curves, and the distance between the two curves for LR = 10. Coatest® APC™ Resistance V and Accelerimat™ were proven to be the methods most able to discriminate for factor V:Q506, while normalization was not shown to improve the screening performance. The original method from Chromogenix was confirmed to undergo many influences (factor XII, PAI-1, thrombin- antithrombin complexes, antithrombin III, hematocrit). Although a very good improvement was provided by the newest methods, they were shown to be influenced by protein S and/or factor V levels in the sample plasma.

QUANTITATIVE ESTIMATION OF URINARY PREGNANETRIOL, PREGNANETRIOLONE, TETRAHYDRO S AND Δ5-PREGNENETRIOL IN THE INVESTIGATION OF ADRENOCORTICAL FUNCTION

1969 ◽  
Vol 60 (4) ◽  
pp. 657-668 ◽  
Author(s):  
Frances J. Thomas ◽  
A. W. Steinbeck
Keyword(s):  
Congenital Adrenal Hyperplasia ◽  
Cushing’S Syndrome ◽  
Adrenocortical Carcinoma ◽  
Quantitative Estimation ◽  
Cushing's Syndrome ◽  
Adrenocortical Function ◽  
Adrenal Hyperplasia ◽  
Ectopic Acth ◽  
Modified Method ◽  
Idiopathic Hirsutism

ABSTRACT A modified method for the estimation of urinary pregnanetriol, pregnanetriol, pregnanetriolone, Δ5-pregnenetriol and tetrahydro S was investigated. The steroids, separated by chromatography, were measured quantitatively, tetrahydro S by reaction with blue tetrazolium and the other three as acetaldehydogenic substances. The excretion of these steroids was studied in suspected instances of adrenal and/or ovarian disease. Urinary pregnanetriol and tetrahydro S levels were normal in Cushing's syndrome, secondary adrenocortical carcinoma, the Stein-Leventhal syndrome, idiopathic hirsutism and hypertension. Tetrahydro S was doubtfully elevated in an instance of ectopic ACTH Cushing's syndrome. Pregnanetriol excretion was elevated in untreated cases of congenital adrenal hyperplasia and after treatment in some. Pregnanetriolone was found in all patients with congenital adrenal hyperplasia, including those receiving cortisone. Pregnanetriolone was also detected in Cushing's syndrome, secondary adrenocortical carcinoma, the Stein-Leventhal syndrome, ectopic ACTH Cushing's syndrome and suspected congenital adrenal hyperplasia. Δ5-pregnenetriol was present as a glucuronide in the Stein-Leventhal syndrome and idiopathic hirsutism. The diagnostic implications of these results are discussed.

Reduction of Pixel Occurrences in Background Updating

2012 ◽  
Vol 17 (4) ◽  
pp. 45-50
Author(s):  
Zbigniew Bubliński ◽  
Piotr Pawlik
Keyword(s):  
Moving Objects ◽  
Time Rate ◽  
Generation Algorithm ◽  
Modified Method ◽  
Background Updating

Abstract The paper presents the modification of background generation algorithm based on analysis of the frequency of occurrences of pixels. The proposed solution allows the generation of the background and its updating, the introduced parameter allows to adjust the algorithm according to the time rate of changes in the image. The results show that the modified method can be applied in many tasks related to the detection and analysis of moving objects.

A modified method to design the LTE authentication protocol EPS-AKA algorithm using the query vector

2019 ◽  
Vol 15 (4) ◽  
pp. 784-794
Author(s):  
N.V. Kormil'tsev ◽  
◽  
A.D. Uvarov ◽  
A.A. Mikhailov ◽  
M.V. Tumbinskaya ◽  
...  
Keyword(s):  
Authentication Protocol ◽  
Modified Method

Comminuted patella fracture in a patient of advanced age, with xeroderma, treated with a low-invasive, originally modified method using non-absorbable sutures

2019 ◽  
Vol 84 (2) ◽  
pp. 51-55
Author(s):  
Jan Poszepczyński ◽  
◽  
Andrzej Kaźmierczyk ◽  
Krzysztof Andrzejewski ◽  
Michał Krzymiński ◽  
...  
Keyword(s):  
Advanced Age ◽  
Patella Fracture ◽  
Modified Method ◽  
Absorbable Sutures

LYCOPENE POWDER PRODUCING FROM GAC FRUIT TOWARDS APPLICATIONS IN NUTRACEUTICAL AND COSMETICS

2016 ◽  
pp. 51-56
Author(s):  
Thi Le Thuy Nguyen ◽  
Thanh Sinh Do ◽  
Thi Hoa Tran
Keyword(s):  
Key Words ◽  
Propylene Glycol ◽  
Mass Production ◽  
Toxic Substances ◽  
High Concentration ◽  
Concentration Determination ◽  
Modified Method ◽  
Β Carotene ◽  
Key Words Carotenoids

Backgroud: Gac is a fruit containing many antioxidants, especially lycopene, with high concentration. However the variety and value of products from gac are limited. We introduce a modified method to produce lycopene and others carotenoids from gac oil towards available and safe applications in nutraceutical and cosmetics. Materials and method: We study the extraction with different parameters and limit at the saponification of gac oil using less toxic substances such as ethanol, propylene glycol, postasium hydroxide and sodium chlorid. Results: Concentrations of lycopene and β-carotene determined silmutaneously by UVVis spectrophotometer present that the efficiency reaches 65.07% as 20 g of gac oil is saponified with 8 g KOH 12 mol/L in 120 mins. The purity of product is 89.02%. Conclusions: This process could minimize toxic residue in the powder after the precipitation, filtration and washing. Besides, the method is stable and applicable to the mass production. Key words: Carotenoids, Lycopene, safe extraction, UV Vis silmutaneous concentration determination

Modified method of detecting DDoS attacks based on entropy

2010 ◽  
Vol 30 (7) ◽  
pp. 1778-1781 ◽  
Author(s):  
Jie ZHANG ◽  
Zheng QIN
Keyword(s):  
Ddos Attacks ◽  
Modified Method
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