ChemInform Abstract: Synthesis of Enantiomerically Pure γ-Amino-β-hydroxybutyric Acid Using Malic Acid as the Chiral Precursor.

1986 ◽  
Vol 17 (25) ◽  
Author(s):  
B. RAJASHEKHAR ◽  
E. T. KAISER
Keyword(s):  
Malic Acid ◽  
Hydroxybutyric Acid ◽  
Enantiomerically Pure

Poly(β-L-malate) hydrolase from Plasmodia of Physarum polycephalum

1995 ◽  
Vol 41 (13) ◽  
pp. 192-199 ◽  
Author(s):  
Christian Korherr ◽  
Michael Roth ◽  
Eggehard Holler
Keyword(s):  
Hydrophobic Interaction ◽  
Malic Acid ◽  
Physarum Polycephalum ◽  
Culture Medium ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Reduced Form ◽  
Serine Esterase ◽  
Hydroxybutyric Acid ◽  
Ph Optimum

A 68-kDa extracellular glycoprotein from Physarum polycephalum that hydrolyses specifically poly(β-L-malic acid) by removing monomers of L-malic acid in an exolytic manner has been purified and characterized. The enzyme was purified 1740-fold from the culture medium by ammonium sulfate precipitation, hydrophobic interaction chromatography on butyl-Toyopearl, and gel permeation chromatography on Superdex 200 to a specific activity of 9.0 μmol∙min−1∙mg−1. The hydrolase was also purified from the cytosol, which contained 1 mg in 43 g cells in contrast to 1 mg extracellular enzyme in 28 L of culture medium. The pH optimum was pH 3.5 as a result of the effect of an acidic side chain on Vmax and the preferred binding of poly(β-L-malate) in the ionized form. Intracellular hydrolase was only marginally active on [14C]poly(β-L-malate) that had been injected into plasmodia. Poly(L-aspartate), poly(L-glutamate), poly(vinyl sulfate), and poly(acrylate) were neither bound nor degraded by the hydrolase. Poly(β-hydroxybutyric acid), which was considered the reduced form of poly(β-L-malate), was not a substrate. The enzyme is neither a metallo- nor a serine-esterase, and is distinct from poly(3-hydroxybutyric acid) depolymerases. It is related to a glucosidase with respect to hydrophobic interaction chromatography, the pH-activity dependence, and its inhibition with mercuribenzoate, N-bromosuccinimide, and D-gluconolactone, but not the use of the substrates.Key words: poly(β-L-malate), polymalatase, Physarum polycephalum, biodegradative polymer.

Tu1474 Reduced 2-Hydroxybutyric Acid and Increased Malic Acid Correlate With Diabetes Remission After Roux-en-Y Gastric Bypass

2015 ◽  
Vol 148 (4) ◽  
pp. S-901-S-902
Author(s):  
Wei-Chih Liao ◽  
Ching-Hua Kuo ◽  
San-Yuan Wang ◽  
Chi-Ming Tai ◽  
Yufeng J Tseng ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Malic Acid ◽  
Diabetes Remission ◽  
Hydroxybutyric Acid

ChemInform Abstract: Regio- and Stereocontrolled Hydrocyanation of Chiral 2-Alkylglycidamides with Et2AlCN: Synthesis of Enantiomerically Pure Mono- and Disubstituted Malic Acid Derivatives.

ChemInform ◽  
2010 ◽  
Vol 33 (49) ◽  
pp. no-no
Author(s):  
Jose L. Garcia Ruano ◽  
M. Angeles Fernandez-Ibanez ◽  
Ana M. Martin-Castro ◽  
Jesus H. Rodriguez Ramos ◽  
Ana C. Rubio Flamarique
Keyword(s):  
Malic Acid ◽  
Enantiomerically Pure
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