ChemInform Abstract: STUDIES ON METAL CARBOXYLATES. 13. A HIGH-YIELD SYNTHESIS OF RHENIUM(III) IODIDE AND THE ISOLATION AND CHARACTERIZATION OF β-MOLYBDENUM(II) IODIDE

1978 ◽  
Vol 9 (15) ◽  
Author(s):  
H. D. GLICKSMAN ◽  
R. A. WALTON
Keyword(s):  
High Yield ◽  
Metal Carboxylates ◽  
Isolation And Characterization

Nine- and Eight-Vertex Polyhedral Dicarbaborane Chemistry: New arachno and hypho Dicarbaboranes from arachno-4,5-C2B7H13: Isolation and Characterization of the [arachno-4,5-C2B7H12]- and [hypho-7,8-C2B6H13]- Anions, and of the Neutral Ligand Derivatives exo-6-(MeNC)-arachno-4,5-C2B7H11 and exo-5-L-hypho-4,9-C2B7H13 (L = NMe3 and NEt3)

1994 ◽  
Vol 59 (7) ◽  
pp. 1584-1595 ◽  
Author(s):  
Tomáš Jelínek ◽  
Josef Holub ◽  
Bohumil Štíbr ◽  
Xavier L. R. Fontaine ◽  
John D. Kennedy
Keyword(s):  
Nmr Spectroscopy ◽  
Selective Removal ◽  
High Yield ◽  
Two Dimensional ◽  
Neutral Ligand ◽  
Tertiary Amines ◽  
Proton Sponge ◽  
H Nmr ◽  
Isolation And Characterization

Deprotonation of neutral arachno-4,5-C2B7H13 (1) either with 1, 8-(NMe2)2C10H6 (proton sponge, PS) or with a mixture of aqueous K2CO3 and [NMe4]Cl leads to the isolation in high yield of the [arachno-4,5-C2B7H12]- anion (2). Isostructural with this anion is the ligand derivative exo-6-(MeNC)-arachno-4,5-C2B7H11 (3), which is prepared in 20% yield from the reaction between arachno-4,5-C2B7H13 and MeNC in dichloromethane. Under comparable conditions compound 1 with tertiary amines gives the first representatives of the nine-vertex hypho family of dicarbaboranes, the ligand derivatives exo-5-(NR3)-hypho-4,9-C2B7H13 (4a and 4b, where R = Me and Et, respectively) in moderate yields (20 - 55%), whereas the reaction between 1 and aqueous NaCN results in the selective removal of one boron vertex to yield the eight-vertex [hypho-7,8-C2B6H13]- anion (5) in 61% yield. All compounds isolated were characterized by 11B and 1H NMR spectroscopy, with two-dimensional and selective decoupling techniques giving unambiguous assignments.

Isolation and characterization of glioblastoma nuclei and chromosomes in the lyophilized state

1971 ◽  
Vol 34 (3) ◽  
pp. 301-309 ◽  
Author(s):  
Wolff M. Kirsch ◽  
Demoy Schulz ◽  
Paul Nakane ◽  
Robert Lasher ◽  
Tadami Yamamoto
Keyword(s):  
Water Soluble ◽  
High Yield ◽  
Nonaqueous Media ◽  
Biosynthetic Activity ◽  
Content Type ◽  
Isolation And Characterization ◽  
Subcellular Organelle ◽  
Fine Print

✓ Intact lyophilized nuclei and chromosomes were obtained from glioblastomas or brain, either in situ or in culture, by freezing at −156°C, drying at −25°C, and mechanical disassociation in glycerol at 2°C. Nuclear or chromosomal isolation was accomplished in hygroscopic nonaqueous media of high density. The method gave homogeneous nuclear and chromosomal preparations in high yield with preservation of labile, water-soluble constituents and residual biosynthetic activity. Unique opportunities for quantitative cytochemical studies at the level of the subcellular organelle are made available by the method.

Isolation and Characterization of the Photoalteration Products of cis- and trans-Chlordane

1975 ◽  
Vol 58 (1) ◽  
pp. 6-9
Author(s):  
Francis I Onuska ◽  
Michael E Comba
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Thin Layer ◽  
High Yield ◽  
Isolation And Characterization ◽  
The Individual ◽  
Cis And Trans ◽  
Layer Chromatography

Abstract Ultraviolet irradiation of cis- and trares-chlordane yielded 3 photolysis products. The expected half-caged analog of cis-chlordane was formed in high yield, and 2 minor photoproducts of trans-chlordane were observed. One of these products was a half-caged isomer. The individual photoproducts were isolated by thin layer chromatography and characterized by infrared, nuclear magnetic resonance, and mass spectrometry.

scholarly journals Isolation and characterization of an endo-β-galactosidase from Bacteroides fragilis

1983 ◽  
Vol 213 (2) ◽  
pp. 485-494 ◽  
Author(s):  
P Scudder ◽  
K Uemura ◽  
J Dolby ◽  
M N Fukuda ◽  
T Feizi
Keyword(s):  
Bacteroides Fragilis ◽  
High Yield ◽  
Keratan Sulphate ◽  
Common Structure ◽  
Isolation And Characterization ◽  
The Common ◽  
Beta Galactosidase

Six strains of Bacteroides fragilis were examined and all found to produce endo-beta-galactosidase, an enzyme that hydrolyses internal β-galactosidic linkages of oligosaccharides belonging to the poly-N-acetyl-lactosamine series, with the common structure GlcNAc β 1 leads to 3Gal β 1 leads to 4GlcNAc/Glc. The enzyme was produced without the addition of an inducer such as keratan sulphate. It was purified 7000-fold from the culture supernatant and obtained with a yield 4-10-fold greater than from sources described previously. The specificity of the enzyme towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide closely resembled that of the endo-beta-galactosidase isolated from Escherichia freundii. A novel observation was that both enzymes hydrolysed the type 2 sequence, Gal β 1 leads to 4GlcNAc β 1 leads to 3Gal β 1 leads to 4Glc, at about twice the rate of the type 1 isomer, Gal β 1 leads to 3GlcNAc β 1 leads to 3Gal β 1 leads to 4Glc. Because of the ease of purification of the enzyme and high yield in the absence of contaminating glycosidases and proteinases, Bacteroides fragilis is a valuable source of endo-beta-galactosidase for the structural analysis of carbohydrate chains.

o-Nitrophenylethylene glycol as photoremovable protective group for aldehydes and ketones: syntheses, scope, and limitations

1983 ◽  
Vol 61 (2) ◽  
pp. 400-410 ◽  
Author(s):  
D. Gravel ◽  
J. Hebert ◽  
D. Thoraval
Keyword(s):  
Point Of View ◽  
High Yield ◽  
Protective Group ◽  
Isolation And Characterization ◽  
Usual Manner ◽  
Aldehydes And Ketones ◽  
Inert Solvent ◽  
Acidic Conditions ◽  
High Yields

The preparation of o-nitrophenylethylene glycol is described along with its application as a photolabile protective group for aldehydes and ketones. Formation of the acetals and ketals is achieved in good to high yields in the usual manner and deprotection is carried out in fair to high yield, by photolysis at 350 nm in an inert solvent such as benzene. Because of the particular nature of the present protective group, its stability to basic and acidic conditions has been examined and is reported to complete the scope and limitations aspect. From a mechanistic point of view, the isolation and characterization of o-nitroso-α-hydroxyacetophenone as the spent reagent demonstrates a mechanistic link with the known o-nitrobenzaldehyde to o-nitrosobenzoic acid photorearrangement.

scholarly journals Isolation and characterization of Ty1-copia retrotransposons in Saccharum officinarum

2018 ◽  
Author(s):  
Ke Chen ◽  
Fan Yu ◽  
Yongji Huang ◽  
Xianglin Wu ◽  
Shan Yang ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Saccharum Officinarum ◽  
Plant Genome ◽  
High Yield ◽  
Dot Blot ◽  
Chromosomal Distribution ◽  
Breeding Programs ◽  
Sugarcane Breeding ◽  
Isolation And Characterization

Background. Saccharum officinarum is the most significant resource for sugar and high-yield genes in sugarcane breeding programs. However, the unknown information of evolution and genome organization remain largely in the sugarcane, which has limited progress in sugarcane breeding. Retrotransposons occupy a large proportion of the plant genome; therefore, characterization of Ty1-copia retrotransposons will improve understanding of the evolution and organization of plant genomes. Methods. The present study isolated conserved domains of Ty1-copia retrotransposon-encoded reverse transcriptase genes from S. officinarum to characterize their phylogenetic diversity, genomic abundance, and chromosomal distribution. Results. In total, 42 Ty1-copia reverse transcriptase sequences with 35-100% similarity and high levels of heterogeneity were obtained. Of them, 11 (26%) were disrupted by stop codons and/or frameshift mutations. Phylogenetic analysis revealed these sequences could be split into four distinct evolutionary lineages (Tork/TAR, Tork/Angela, Sire/Maximus, and Retrofit/Ale). Dot blot analysis showed that Ty1-copia retrotransposons represent a significant portion of the S. officinarum genome, with copy numbers as high as 1.7 × 105. Fluorescence in situ hybridization revealed that Ty1-copia retrotransposons were dispersed within heterochromatic regions among all S. officinarum chromosomes, with around 30 obvious signals clustering in terminal regions. However, Ty1-copia retrotransposons were not found in nucleolar organizing regions of 45S rDNA. Discussion. These results serve to enhance our understanding of the chromosomal distribution and evolution of the S. officinarum genome as well as promote possible utilization of retrotransposons in sugarcane breeding programs.

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