Full‐Atom Model of the Agonist LPS‐bound Toll‐like Receptor 4 Dimer in a Membrane Environment

Background: The purpose of this study was to determine whether myeloid differentiation factor88-dependent Toll-Like Receptor-4 (TLR-4) signaling contributed to the inhibition of abdominal aortic aneurysm (AAA) by Tanshinone IIA (Tan IIA). Materials and methods: Male Sprague-Dawley rats (n = 12 / group) were randomly distributed into three groups: Tan IIA, control, and sham. The rats from Tan IIA and control groups under-went intra-aortic elastase perfusion to induce AAAs, and those in the sham group were perfused with saline. Only the Tan IIA group received Tan IIA (2 mg / rat / d). Aortic tissue samples were harvested at 24 d after perfusion and evaluated using reverse transcriptase-polymerase chain reaction, Western blot, immunohistochemistry and immunofluorescence. Results: The over-expression of Toll-Like Receptor-4 (TLR-4), Myeloid Differentiation factor 88 (MyD88), Phosphorylated Nuclear Factor κB (pNF-κB) and Phosphorylated IκBα (pIκBα) induced by elastase perfusion were significantly decreased by Tan IIA treatment. Conclusions: Tan IIA attenuates elastase-induced AAA in rats possibly via the inhibition of MyD88-dependent TLR-4 signaling, which may be one potential explanation of why Tan IIA inhibits AAA development through multiple effects.

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An Immunomodulating Polysaccharide in Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae) Activates Macrophages through Toll-like Receptor 4

International Journal of Medicinal Mushrooms ◽

10.1615/intjmedmushr.v8.i3.40 ◽

2006 ◽

Vol 8 (3) ◽

pp. 223-229 ◽

Cited By ~ 5

Author(s):

Masashi Mizuno ◽

Sachiko Kawakami

Keyword(s):

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Agaricus Brasiliensis

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Genetic Polymorphism of Toll-Like Receptor 4 Thr399Ile Variant in Iraqi Kurdish Population: Sulaymaniyah Province

passer ◽

10.24271/psr.08 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Sherko Niranji

Keyword(s):

Genetic Polymorphism ◽

Toll Like Receptor ◽

Toll Like Receptor 4

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Association of the CD14 C159T and the Toll-like receptor 4 Asp299Gly polymorphisms with various phenotypes of asthma in adults from Crimea

Allergy and Asthma Proceedings ◽

10.2500/aap.2019.40.190007 ◽

2020 ◽

Vol 41 (2) ◽

pp. 134-140

Author(s):

Yuriy Bisyuk ◽

Andrew Dubovyi ◽

Ilona DuBuske ◽

Viktor Litus ◽

Lawrence M. DuBuske

Keyword(s):

Late Onset ◽

Adult Population ◽

Additive Models ◽

Atopic Asthma ◽

Control Group ◽

Nucleotide Polymorphisms ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Single Nucleotide ◽

Gender And Age

Background: This study assessed gene polymorphisms of the CD14 receptor (C-159T) and Toll-like receptor 4 (Asp299Gly) in a patient population in Crimea, Ukraine, stratified by clinical (early versus late onset; frequent versus occasional relapses; fixed versus reversible obstruction) and immunologic (atopic versus nonatopic; eosinophilic; neutrophilic or paucigranulocytic inflammation) subtype. Methods: Two polymorphisms, CD14 C-159T and TLR4 Asp299Gly, were assessed in 331 patients with asthma. The control group included 285 volunteers who were nonatopic. The single nucleotide polymorphisms were studied by using polymerase chain reaction with electrophoretic detection. Results: There were increased odds of asthma development in patients with the Asp299Gly TLR4 mutation compared with the general population underdominant odds ratio (OR) 1.52 [95% confidence interval (CI), 1.00‐2.32] and overdominant (OR 1.55 [95% CI, 1.01‐2.38]) models after adjustment for gender and age. In addition, mutations in this gene decreased the odds of nonatopic asthma in underdominant (OR 0.26 [95% CI, 0.07‐0.93]; p = 0.027), overdominant (OR 0.27 [95% CI, 0.07‐0.96]; p = 0.033), and log-additive models (OR 0.26 [95% CI, 0.07‐0.93]; p = 0.026) compared with the atopic subgroup after adjustment for gender, age, number of exacerbations, and type of airway inflammation. Allele frequencies for CD14 and TLR4 polymorphisms did not show statistical differences between the patients with asthma and the control subjects. Conclusion: CD14 C-159T polymorphisms were not associated with asthma in the adult population in Crimea. TLR4 Asp299Gly polymorphisms were associated with asthma and with decreased odds of nonatopic asthma compared with atopic asthma in the adult population in Crimea.

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