scholarly journals Frontispiece: Light‐Driven Active Ion Transport

2020 ◽  
Vol 26 (61) ◽  
Author(s):  
Jinlei Yang ◽  
Pengchao Liu ◽  
Lianshan Li ◽  
Zhiyong Tang
Keyword(s):  
Ion Transport ◽  
Active Ion Transport

Basolateral Cl− uptake mechanisms in Xenopus laevis lung epithelium

2010 ◽  
Vol 299 (1) ◽  
pp. R92-R100 ◽  
Author(s):  
Jens Berger ◽  
Martin Hardt ◽  
Wolfgang G. Clauss ◽  
Martin Fronius
Keyword(s):  
Xenopus Laevis ◽  
Ion Transport ◽  
Anion Exchanger ◽  
Short Circuit ◽  
Ussing Chamber ◽  
Short Circuit Current ◽  
Transport Processes ◽  
Lung Epithelium ◽  
Active Ion Transport ◽  
Uptake Mechanisms

A thin liquid layer covers the lungs of air-breathing vertebrates. Active ion transport processes via the pulmonary epithelial cells regulate the maintenance of this layer. This study focuses on basolateral Cl− uptake mechanisms in native lungs of Xenopus laevis and the involvement of the Na+/K+/2 Cl− cotransporter (NKCC) and HCO3−/Cl− anion exchanger (AE), in particular. Western blot analysis and immunofluorescence staining revealed the expression of the NKCC protein in the Xenopus lung. Ussing chamber experiments demonstrated that the NKCC inhibitors (bumetanide and furosemide) were ineffective at blocking the cotransporter under basal conditions, as well as under pharmacologically stimulated Cl−-secreting conditions (forskolin and chlorzoxazone application). However, functional evidence for the NKCC was detected by generating a transepithelial Cl− gradient. Further, we were interested in the involvement of the HCO3−/Cl− anion exchanger to transepithelial ion transport processes. Basolateral application of DIDS, an inhibitor of the AE, resulted in a significantly decreased the short-circuit current (ISC). The effect of DIDS was diminished by acetazolamide and reduced by increased external HCO3− concentrations. Cl− secretion induced by forskolin was decreased by DIDS, but this effect was abolished in the presence of HCO3−. These experiments indicate that the AE at least partially contributes to Cl− secretion. Taken together, our data show that in Xenopus lung epithelia, the AE, rather than the NKCC, is involved in basolateral Cl− uptake, which contrasts with the common model for Cl− secretion in pulmonary epithelia.

Membrane dynamics in insect malpighian tubules

1989 ◽  
Vol 257 (5) ◽  
pp. R967-R972
Author(s):  
T. J. Bradley
Keyword(s):  
Ion Transport ◽  
Driving Force ◽  
Fluid Secretion ◽  
Membrane Dynamics ◽  
Malpighian Tubules ◽  
Membrane Insertion ◽  
Cation Pump ◽  
Active Ion Transport ◽  
Urine Formation ◽  
Apical Membranes

Urine formation in insects occurs in the Malpighian tubules by means of active ion transport and osmotically coupled water flow. The rates of urine formation can vary with time and can be modulated by diuretic hormones, developmental events, and intracellular parasitism. This paper reviews a number of recent studies in which it has been demonstrated that variations in transport rate are associated with substantial changes in tubule ultrastructure in the form of membrane insertion into and deletion from the apical microvilli. The principal driving force for fluid movement in Malpighian tubules is thought to be a common cation pump located in the apical membranes. It is proposed that modulation of the apical microvillar membrane may reflect regulation by the cells of the number of common cation pump units involved in fluid secretion.

Active Ion Transport by Mitochondria

1967 ◽  
pp. 90-94
Author(s):  
Carlo S. Rossi ◽  
Ernesto Carafoli ◽  
Albert L. Lehninger
Keyword(s):  
Ion Transport ◽  
Active Ion Transport

Photodriven Active Ion Transport Through a Janus Microporous Membrane

2020 ◽  
Vol 59 (15) ◽  
pp. 6244-6248 ◽  
Author(s):  
Jinlei Yang ◽  
Pengchao Liu ◽  
Xiao He ◽  
Junjun Hou ◽  
Yaping Feng ◽  
...  
Keyword(s):  
Ion Transport ◽  
Microporous Membrane ◽  
Active Ion Transport

Photodriven Active Ion Transport Through a Janus Microporous Membrane

2020 ◽  
Vol 132 (15) ◽  
pp. 6303-6307 ◽  
Author(s):  
Jinlei Yang ◽  
Pengchao Liu ◽  
Xiao He ◽  
Junjun Hou ◽  
Yaping Feng ◽  
...  
Keyword(s):  
Ion Transport ◽  
Microporous Membrane ◽  
Active Ion Transport
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