scholarly journals Structure‐Guided Design of a Group B Streptococcus Type III Synthetic Glycan–Conjugate Vaccine

2020 ◽  
Vol 26 (31) ◽  
pp. 6944-6944 ◽  
Author(s):  
Davide Oldrini ◽  
Linda Bino ◽  
Ana Arda ◽  
Filippo Carboni ◽  
Pedro Henriques ◽  
...  
Keyword(s):  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Type Iii ◽  
Group B

scholarly journals Estimation of Group B Streptococcus Type III Polysaccharide-Specific Antibody Concentrations in Human Sera Is Antigen Dependent

1998 ◽  
Vol 66 (12) ◽  
pp. 5848-5853 ◽  
Author(s):  
Reva Bhushan ◽  
Bascom F. Anthony ◽  
Carl E. Frasch
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Antigenic Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type Iii ◽  
Human Sera ◽  
Group B

ABSTRACT The presence of immunoglobulin G (IgG) antibodies against group B streptococcus (GBS) type III polysaccharide (PS) has been correlated with protection against GBS disease. The GBS type III PS is structurally similar to the pneumococcal type 14 PS, differing only in the presence of sialic acid residues. Four different preparations of GBS type III PS were evaluated for their specificity in enzyme-linked immunosorbent assay (ELISA): free PS, free PS mixed with methylated human serum albumin (mHSA), PS conjugated to biotin and PS conjugated to human serum albumin. Three groups of human sera were used to evaluate these PS preparations: sera from recipients of a GBS PS vaccine, sera from women receiving a GBS type III PS-tetanus toxoid conjugate vaccine, and sera from nonimmunized healthy women of childbearing age. Estimated antibody concentrations were different depending on the PS preparation used. Using any of the four preparations, we were able to measure ≤0.05 μg of IgG antibody to the GBS type III PS per ml. The specificity of the assay was determined by competitive inhibition with homologous and heterologous PS. The pneumococcal type 14 PS did not inhibit binding of antibody to the native GBS type III PS in sera from adults receiving the GBS PS vaccine or in sera from nonimmunized adults (except serum G9). The pneumococcal type 14 PS inhibited 50% in sera from recipients of GBS type III conjugate vaccine and in serum G9 when GBS type III PS conjugated to biotin or to HSA was used as antigen in ELISA. These data show that free GBS type III PS or PS mixed with mHSA is a sensitive and specific antigen for ELISA and that conjugation can alter the antigenic specificity of a PS.

scholarly journals Immunogenicity in animals of a polysaccharide-protein conjugate vaccine against type III group B Streptococcus.

1990 ◽  
Vol 86 (5) ◽  
pp. 1428-1433 ◽  
Author(s):  
M R Wessels ◽  
L C Paoletti ◽  
D L Kasper ◽  
J L DiFabio ◽  
F Michon ◽  
...  
Keyword(s):  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Type Iii ◽  
Protein Conjugate ◽  
Group B

scholarly journals Structure‐Guided Design of a Group B Streptococcus Type III Synthetic Glycan–Conjugate Vaccine

2020 ◽  
Vol 26 (31) ◽  
pp. 7018-7025 ◽  
Author(s):  
Davide Oldrini ◽  
Linda Bino ◽  
Ana Arda ◽  
Filippo Carboni ◽  
Pedro Henriques ◽  
...  
Keyword(s):  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Type Iii ◽  
Group B

scholarly journals An oligosaccharide-tetanus toxoid conjugate vaccine against type III group B Streptococcus.

1990 ◽  
Vol 265 (30) ◽  
pp. 18278-18283
Author(s):  
L C Paoletti ◽  
D L Kasper ◽  
F Michon ◽  
J DiFabio ◽  
K Holme ◽  
...  
Keyword(s):  
Conjugate Vaccine ◽  
Tetanus Toxoid ◽  
Group B Streptococcus ◽  
Type Iii ◽  
Group B

scholarly journals Front Cover: Structure‐Guided Design of a Group B Streptococcus Type III Synthetic Glycan–Conjugate Vaccine (Chem. Eur. J. 31/2020)

2020 ◽  
Vol 26 (31) ◽  
pp. 6940-6940
Author(s):  
Davide Oldrini ◽  
Linda Bino ◽  
Ana Arda ◽  
Filippo Carboni ◽  
Pedro Henriques ◽  
...  
Keyword(s):  
Conjugate Vaccine ◽  
Group B Streptococcus ◽  
Front Cover ◽  
Type Iii ◽  
Group B

Group B Streptococcal and Pneumococcal Sepsis in Newborn Infants: The Role of Pneumococcal Antibody

PEDIATRICS ◽  
1978 ◽  
Vol 62 (4) ◽  
pp. 620-621
Author(s):  
Gerald W. Fischer ◽  
James W. Bass ◽  
George H. Lowell ◽  
Martin H. Crumrine
Keyword(s):  
Streptococcus Pneumoniae ◽  
Hydrochloric Acid ◽  
Group B Streptococcus ◽  
Newborn Infants ◽  
Polysaccharide Antigen ◽  
Type Iii ◽  
Pneumococcal Sepsis ◽  
Group B

The article by Bortolussi et al. on pneumococcal septicemia and meningitis in the neonat (Pediatrics 60:352, September 1977) was of great interest to us, since we have been analyzing the effect of antibody directed against Streptococcus pneumoniae on group B Streptococcus type III. We have recently shown (unpublished data) that antibody directed against S. pneumoniae type 14 precipitates the hot hydrochloric acid-extracted polysaccharide antigen of group B Streptococcus type III. Further studies have shown that this antibody is opsonic for group B Streptococcus type III in an in vitro bactericidal assay and protective in a suckling rat model of group B Streptococcus type III sepsis.1

Colonization With Group B Streptococci in Girls Under 16 Years of Age

PEDIATRICS ◽  
1977 ◽  
Vol 60 (4) ◽  
pp. 473-476 ◽  
Author(s):  
Margaret R. Hammerschlag ◽  
Carol J. Baker ◽  
Susan Alpert ◽  
Dennis L. Kasper ◽  
Ingrid Rosner ◽  
...  
Keyword(s):  
Anal Canal ◽  
Serum Antibody ◽  
Group B Streptococcus ◽  
Group B Streptococci ◽  
Capsular Antigen ◽  
Type Iii ◽  
Group B

Cultures from the vagina, pharynx, and anal canal of 100 healthy girls, 2 months through 15 years of age, were examined for the presence of group B streptococci. Of the 100 participants, 20% were colonized at one or more of these three sites. Pharyngeal colonization was detected in 15% of the girls under 11 years of age and in 5% of those over 11 years of age. Colonization at anogenital sites was observed in 19% of participants under 3 years of age, in 25% of those 11 years of age and older, and in only 4% of those between the ages of 3 and 10 years (P < .025). The concentration of serum antibody directed against the polysaccharide capsular antigen isolated from type III, group B Streptococcus appeared, in part, to be related to increasing age.

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