Switchable Enzymatic Accessibility for Precision Cell‐Selective Surface Glycan Remodeling

2019 ◽  
Vol 25 (44) ◽  
pp. 10505-10510 ◽  
Author(s):  
Peiwen Zhang ◽  
Yiran Li ◽  
Xiaofei Yu ◽  
Huangxian Ju ◽  
Lin Ding
Keyword(s):  
Enzymatic Accessibility

scholarly journals Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 507
Author(s):  
Eduardo Troncoso-Ortega ◽  
Rosario del P. Castillo ◽  
Pablo Reyes-Contreras ◽  
Patricia Castaño-Rivera ◽  
Regis Teixeira Mendonça ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Eucalyptus Globulus ◽  
Steam Explosion ◽  
Laser Scanning ◽  
Structural Changes ◽  
Micro Particles ◽  
Cellulose Accessibility ◽  
Ft Ir ◽  
Enzymatic Accessibility ◽  
Physical And Chemical

The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

scholarly journals Unrevealing model compounds of soil conditioners impacts on the wheat straw autohydrolysis efficiency and enzymatic hydrolysis

2020 ◽  
Author(s):  
Xinxing Wu ◽  
Wei Tang ◽  
Chen Huang ◽  
Caoxing Huang ◽  
Chenhuan Lai ◽  
...  
Keyword(s):  
Organic Matter ◽  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Sodium Phosphate ◽  
Soluble Salts ◽  
Model Compounds ◽  
Enzymatic Digestibility ◽  
Sodium Humate ◽  
Soil Conditioners ◽  
Enzymatic Accessibility

Abstract Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS autohydrolysis and enzymatic hydrolysis.

Enzymatic accessibility of xylans in lignocellulosic materials

1994 ◽  
Vol 41 (1) ◽  
pp. 124-129 ◽  
Author(s):  
Liisa Viikari ◽  
Anne Kantelinen ◽  
Johanna Buchert ◽  
J�rgen Puls
Keyword(s):  
Lignocellulosic Materials ◽  
Enzymatic Accessibility

Enzymatic accessibility of xylans in lignocellulosic materials

1994 ◽  
Vol 41 (1) ◽  
pp. 124-129 ◽  
Author(s):  
Liisa Viikari ◽  
Anne Kantelinen ◽  
Johanna Buchert ◽  
J�rgen Puls
Keyword(s):  
Lignocellulosic Materials ◽  
Enzymatic Accessibility
Sign in / Sign up

Export Citation Format

Share Document