A Highly Selective Fluorescent Probe for Detection of Hydrogen Sulfide in Living Systems: In Vitro and in Vivo Applications

2017 ◽  
Vol 23 (41) ◽  
pp. 9872-9878 ◽  
Author(s):  
Shahi Imam Reja ◽  
Neetu Sharma ◽  
Muskan Gupta ◽  
Payal Bajaj ◽  
Vandana Bhalla ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Living Systems

Ultrafast response fluorescent probe with red-emission for monitoring hydrogen sulfide in vivo and in vitro

2019 ◽  
Vol 382 ◽  
pp. 111974 ◽  
Author(s):  
Han Zhang ◽  
Mei Zhu ◽  
Dao-Yong Jiang ◽  
Xing-Ying Xue ◽  
Jiao Zhang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Red Emission

Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Cell Model ◽  
Site Directed Mutagenesis ◽  
Critical Event ◽  
Hypertensive Rats ◽  
Pulmonary Artery Endothelial Cell

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.

scholarly journals H2S in acute lung injury: a therapeutic dead end(?)

2020 ◽  
Vol 8 (S1) ◽  
Author(s):  
Tamara Merz ◽  
Nicole Denoix ◽  
Martin Wepler ◽  
Holger Gäßler ◽  
David A. C. Messerer ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Circulatory Shock ◽  
Therapeutic Window ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Clinical Reality ◽  
Dead End

AbstractThis review addresses the plausibility of hydrogen sulfide (H2S) therapy for acute lung injury (ALI) and circulatory shock, by contrasting the promising preclinical results to the present clinical reality. The review discusses how the narrow therapeutic window and width, and potentially toxic effects, the route, dosing, and timing of administration all have to be balanced out very carefully. The development of standardized methods to determine in vitro and in vivo H2S concentrations, and the pharmacokinetics and pharmacodynamics of H2S-releasing compounds is a necessity to facilitate the safety of H2S-based therapies. We suggest the potential of exploiting already clinically approved compounds, which are known or unknown H2S donors, as a surrogate strategy.

scholarly journals Hydrogen Sulfide–Releasing Aspirin Derivative ACS14 Exerts Strong Antithrombotic Effects In Vitro and In Vivo

2012 ◽  
Vol 32 (12) ◽  
pp. 2884-2891 ◽  
Author(s):  
Joachim Pircher ◽  
Franziska Fochler ◽  
Thomas Czermak ◽  
Hanna Mannell ◽  
Bjoern F. Kraemer ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Antithrombotic Effects

A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (104) ◽  
pp. 85957-85963 ◽  
Author(s):  
Peng Wang ◽  
Ke Wang ◽  
Dan Chen ◽  
Yibo Mao ◽  
Yueqing Gu
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Near Infrared

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H2O2.

A thiazolo[4,5-b]pyridine-based fluorescent probe for detection of zinc ions and application for in vitro and in vivo bioimaging

Talanta ◽  
2018 ◽  
Vol 185 ◽  
pp. 396-404 ◽  
Author(s):  
Jiao Gong ◽  
Yuan-heng Li ◽  
Chan-juan Zhang ◽  
Jian Huang ◽  
Qi Sun
Keyword(s):  
Fluorescent Probe ◽  
Zinc Ions
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