Metal-Ion-Triggered Exonuclease III Activity for the Construction of DNA Colorimetric Logic Gates

2015 ◽  
Vol 21 (43) ◽  
pp. 15272-15279 ◽  
Author(s):  
Wan Gao ◽  
Li Zhang ◽  
Ru-Ping Liang ◽  
Jian-Ding Qiu
Keyword(s):  
Metal Ion ◽  
Logic Gates ◽  
Exonuclease Iii

scholarly journals Modular logic gates: cascading independent logic gates via metal ion signals

2014 ◽  
Vol 43 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Esra Tanriverdi Ecik ◽  
Ahmet Atilgan ◽  
Ruslan Guliyev ◽  
T. Bilal Uyar ◽  
Aysegul Gumus ◽  
...  
Keyword(s):  
Metal Ion ◽  
Logic Gates ◽  
Modular Logic

Aggregation-induced emission logic gates based on metal ion sensing of phenanthroline–tetraphenylethene conjugates

2013 ◽  
Vol 1 (45) ◽  
pp. 7519 ◽  
Author(s):  
Wen-Liang Gong ◽  
Matthew P. Aldred ◽  
Guo-Feng Zhang ◽  
Chong Li ◽  
Ming-Qiang Zhu
Keyword(s):  
Metal Ion ◽  
Logic Gates ◽  
Aggregation Induced Emission ◽  
Ion Sensing ◽  
Metal Ion Sensing

scholarly journals Highly Sensitive and Selective Copper (II)-Catalyzed Dual-DNAzyme Colorimetric Biosensor Based on Exonuclease III-Mediated Cyclical Assembly

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1352
Author(s):  
Baiqiang Zhai ◽  
Kunlun Huang ◽  
Hongtao Wang ◽  
Dongmin Su ◽  
Yuancong Xu
Keyword(s):  
Metal Ion ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
High Sensitivity ◽  
General Purpose ◽  
Exonuclease Iii ◽  
Dna Cleaving ◽  
Transition Sequence ◽  
Highly Sensitive ◽  
Exo Iii

“Cu-DNAzyme” and “G4-DNAzyme” were used to develop a “turn-off” dual-DNAzyme colorimetric biosensor, which could be used to detect Cu2+ by employing exonuclease III-mediated cyclical assembly (EMCA). EMCA was based on the cleavage activity of Cu2+ to transfer the linkage sequences of the substrate strand and enzyme strand into the transition sequence. The horseradish peroxidase (HRP)-mimicking activity of the G4-DNAzyme was lost after binding with the complementary transition sequence and was hydrolyzed by Exo III. These results demonstrate that the proposed colorimetric biosensor was an effective method for ultradetection of trace metals in a high original signal background. Due to the high sensitivity of the biosensor, the limit of detection (LOD) of Cu2+ is 0.16 nM. This design offers a general purpose platform that could be applied for the detection of any metal ion target through adjustment of metal-dependent DNA-cleaving DNAzymes, which is of great significance for the rapid determination of food safety.

DNA-bound metal ions: recent developments

2014 ◽  
Vol 5 (5) ◽  
pp. 397-407 ◽  
Author(s):  
Daniel L. Morris
Keyword(s):  
Dna Damage ◽  
Metal Ions ◽  
Recent Work ◽  
Conformational Changes ◽  
Metal Ion ◽  
Oxidative Dna Damage ◽  
Logic Gates ◽  
Base Pairing ◽  
Ion Interactions ◽  
Metal Ion Interactions

AbstractThe affinity of metal ions for DNA is logical considering that the structure of DNA includes a phosphate backbone with a net-negative charge, a deoxyribose sugar with O atoms, and purine and pyrimidine bases that contain O and N atoms. DNA-metal ion interactions encompass a large area of research that ranges from the most fundamental characterization of DNA-metal ion binding to the role of DNA-bound metal ions in disease and human health. Alternative DNA base pairing mediated by metal binding is also being investigated and manipulated for applications in logic gates, molecular machines, and nanotechnology. This review highlights recent work aimed at understanding interactions of redox-active metal ions with DNA that provides a better understanding of the mechanisms by which various types of oxidative DNA damage (strand breakage and base modifications) occur. Antioxidants that mitigate oxidative DNA damage by coordinating metal ions that produce reactive oxygen species are addressed, as well as recent work on the effect of DNA-metal ion interactions and the efficacy of quinolone-based antibacterial drugs. Recent advances in metal-mediated base pairing that triggers conformational changes in DNA structure for use as selective metal ion sensors and novel nanotechnology applications are also included.

DNA Electronic Logic Gates Based on Metal-Ion-Dependent Induction of Oligonucleotide Structural Motifs

2013 ◽  
Vol 19 (22) ◽  
pp. 6961-6965 ◽  
Author(s):  
Yun-Mei Zhang ◽  
Li Zhang ◽  
Ru-Ping Liang ◽  
Jian-Ding Qiu
Keyword(s):  
Metal Ion ◽  
Logic Gates ◽  
Structural Motifs ◽  
Electronic Logic

scholarly journals A dipodal molecular probe for naked eye detection of trivalent cations (Al3+, Fe3+and Cr3+) in aqueous medium and its applications in real sample analysis and molecular logic gates

RSC Advances ◽  
2018 ◽  
Vol 8 (63) ◽  
pp. 35946-35958 ◽  
Author(s):  
Rukmani Chandra ◽  
Amit Kumar Manna ◽  
Kalyani Rout ◽  
Jahangir Mondal ◽  
Goutam K. Patra
Keyword(s):  
Metal Ion ◽  
Logic Gates ◽  
Molecular Probe ◽  
Practical Application ◽  
Trivalent Metal ◽  
Real Sample Analysis ◽  
Molecular Logic ◽  
Molecular Logic Gates ◽  
Trivalent Cations ◽  
Naked Eye Detection

A dipodal reversible colorimetric trivalent metal ion chemosensor (L) has been designed and synthesized. The chemosensor L successfully detects Al3+, Fe3+ and Cr3+ based on binding site-signaling approach and it has practical application.

S,N-Co-doped carbon nanoparticles with high quantum yield for metal ion detection, IMP logic gates and bioimaging applications

2018 ◽  
Vol 42 (24) ◽  
pp. 20180-20189 ◽  
Author(s):  
Jinping Song ◽  
Qi Ma ◽  
Sufang Zhang ◽  
Huijun Liu ◽  
Yong Guo ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Carbon Nanoparticles ◽  
Metal Ion ◽  
Logic Gate ◽  
Logic Gates ◽  
High Quantum Yield ◽  
Ion Detection ◽  
High Quantum ◽  
Metal Ion Detection ◽  
Co Doped

S,N-CNPs with high quantum yield exhibited potential multiple applications including metal ion detection, IMP logic gate fabrication and bioimaging.

Electron microscopic assays of restriction endonuclease cutting, exonuclease digestion, and hybridization

1984 ◽  
Vol 42 ◽  
pp. 686-687
Author(s):  
Mark Hannibal ◽  
Jacob Varkey ◽  
Michael Beer
Keyword(s):  
Electron Microscope ◽  
Low Temperature ◽  
Restriction Endonuclease ◽  
Double Helix ◽  
Test Material ◽  
Electron Microscopic ◽  
Exonuclease Iii ◽  
Dna Molecule ◽  
Linear Molecules ◽  
Exonuclease Digestion

Workman and Langmore have recently proposed a procedure for isolating particular chromatin fragments. The method requires restriction endonuclease cutting of the chromatin and a probe, their digestion with two exonucleases which leave complimentary single strand termini and low temperature hybridization of these. We here report simple electron microscopic monitoring of the four reactions involved.Our test material was ϕX-174 RF DNA which is cut once by restriction endonuclease Xho I. The conversion of circles to linear molecules was followed in Kleinschmidt spreads. Plate I shows a circular and a linear DNA molecule. The rate of cutting is shown in Figure 1.After completion of the endonuclease cutting, one portion of the DNA was treated with exonuclease III, an enzyme known to digest the 3' terminals of double helical DNA. Aliquots when examined in the electron microscope reveal a decreasing length of double helix and increasing bushes at the ends.

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