Sequence-Specific DNA Alkylation Targeting for Kras Codon 13 Mutation by Pyrrole-Imidazole Polyamideseco-CBI Conjugates

2013 ◽  
Vol 20 (5) ◽  
pp. 1310-1317 ◽  
Author(s):  
Rhys Dylan Taylor ◽  
Sefan Asamitsu ◽  
Tomohiro Takenaka ◽  
Makoto Yamamoto ◽  
Kaori Hashiya ◽  
...  
Keyword(s):  
Dna Alkylation ◽  
Kras Codon

Mustards-Derived Terpyridine–Platinum Complexes as Anticancer Agents: DNA Alkylation vs Coordination

2021 ◽  
Vol 60 (4) ◽  
pp. 2414-2424
Author(s):  
Muneebah Adams ◽  
Matthew P. Sullivan ◽  
Kelvin K. H. Tong ◽  
David C. Goldstone ◽  
Muhammad Hanif ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Platinum Complexes ◽  
Dna Alkylation

scholarly journals Inhibition of KRAS codon 12 mutants using a novel DNA-alkylating pyrrole–imidazole polyamide conjugate

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Kiriko Hiraoka ◽  
Takahiro Inoue ◽  
Rhys Dylan Taylor ◽  
Takayoshi Watanabe ◽  
Nobuko Koshikawa ◽  
...  
Keyword(s):  
Kras Codon

scholarly journals Colorectal carcinomas with KRAS codon 12 mutation are associated with more advanced tumor stages

BMC Cancer ◽  
2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Wenbin Li ◽  
Tian Qiu ◽  
Wenxue Zhi ◽  
Susheng Shi ◽  
Shuangmei Zou ◽  
...  
Keyword(s):  
Colorectal Carcinomas ◽  
Advanced Tumor ◽  
Tumor Stages ◽  
Kras Codon

A new synthetic analogue of the bracken ultimate carcinogen: Elevation of stability and alteration of DNA alkylation site selectivity

1997 ◽  
Vol 38 (18) ◽  
pp. 3235-3238 ◽  
Author(s):  
Hideo Kigoshi ◽  
Yuusi Kitamura ◽  
Tatsuya Fujita ◽  
Hidekazu Ohashi ◽  
Toshiyuki Atsumi ◽  
...  
Keyword(s):  
Dna Alkylation ◽  
Synthetic Analogue ◽  
Site Selectivity

scholarly journals Genotype-Specific Signal Generation Based on Digestion of 3-Way DNA Junctions: Application to KRAS Variation Detection

2006 ◽  
Vol 52 (10) ◽  
pp. 1855-1863 ◽  
Author(s):  
Giulia Amicarelli ◽  
Daniel Adlerstein ◽  
Erlet Shehi ◽  
Fengfei Wang ◽  
G Mike Makrigiorgos
Keyword(s):  
Nucleic Acid ◽  
Concordance Rate ◽  
Signal Generation ◽  
Sequence Variants ◽  
Wild Type ◽  
Specific Signal ◽  
Novel Technology ◽  
Dna Junctions ◽  
Wide Range ◽  
Kras Codon

Abstract Background: Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-acid (PNA)-based variant enrichment to detect and simultaneously genotype v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) codon 12 sequence variants in human tissue specimens. Materials and Methods: We analyzed KRAS codon 12 sequence variants in 106 lung cancer surgical specimens. We conducted a PNA-PCR reaction that suppresses wild-type KRAS amplification and genotyped the product with a set of OCEAN reactions carried out in fluorescence microplate format. The isothermal OCEAN assay enabled a 3-way DNA junction to form between the specific target nucleic acid, a fluorescently labeled “amplifier”, and an “anchor”. The amplifier-anchor contact contains the recognition site for a restriction enzyme. Digestion produces a cleaved amplifier and generation of a fluorescent signal. The cleaved amplifier dissociates from the 3-way DNA junction, allowing a new amplifier to bind and propagate the reaction. Results: The system detected and genotyped KRAS sequence variants down to ∼0.3% variant-to-wild-type alleles. PNA-PCR/OCEAN had a concordance rate with PNA-PCR/sequencing of 93% to 98%, depending on the exact implementation. Concordance rate with restriction endonuclease-mediated selective-PCR/sequencing was 89%. Conclusion: OCEAN is a practical and low-cost novel technology for sequence-specific signal generation. Reliable analysis of KRAS sequence alterations in human specimens circumvents the requirement for sequencing. Application is expected in genotyping KRAS codon 12 sequence variants in surgical specimens or in bodily fluids, as well as single-base variations and sequence alterations in other genes.

Combination of porphyrins and DNA-alkylation agents: Synthesis and tumor cell apoptosis induction

2006 ◽  
Vol 14 (4) ◽  
pp. 1068-1077 ◽  
Author(s):  
Hanping He ◽  
Yan Zhou ◽  
Feng Liang ◽  
Dongqing Li ◽  
Juanjuan Wu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Apoptosis ◽  
Dna Alkylation ◽  
Apoptosis Induction ◽  
Tumor Cell Apoptosis
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