Multiply Nitrated High-Energy Dense Oxidizers Derived from the Simple Amino Acid Glycine

2013 ◽  
Vol 19 (46) ◽  
pp. 15627-15638 ◽  
Author(s):  
Andreas Baumann ◽  
Anna Erbacher ◽  
Camilla Evangelisti ◽  
Thomas M. Klapötke ◽  
Burkhard Krumm ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Energy ◽  
Simple Amino Acid ◽  
Amino Acid Glycine

scholarly journals B0AT1 amino acid transporter complexed with SARS-CoV-2 receptor ACE2 forms a heterodimer functional unit: in situ conformation using radiation inactivation analysis

Function ◽  
2021 ◽  
Author(s):  
Bruce R Stevens ◽  
J Clive Ellory ◽  
Robert L Preston
Keyword(s):  
Amino Acid ◽  
Membrane Trafficking ◽  
Functional Unit ◽  
Membrane Vesicles ◽  
High Energy ◽  
Amino Acid Transporter ◽  
Neutral Amino Acid ◽  
Transport Activity ◽  
Acid Transporter

Abstract The SARS-CoV-2 receptor, Angiotensin Converting Enzyme-2 (ACE2), is expressed at levels of greatest magnitude in the small intestine as compared to all other human tissues. Enterocyte ACE2 is co-expressed as the apical membrane trafficking partner obligatory for expression and activity of the B0AT1 sodium-dependent neutral amino acid transporter. These components are assembled as an [ACE2: B0AT1]2 dimer-of-heterodimers quaternary complex that putatively steers SARS-CoV-2 tropism in the gastrointestinal (GI) tract. GI clinical symptomology is reported in about half of COVID-19 patients, and can be accompanied by gut shedding of virion particles. We hypothesized that within this 4-mer structural complex, each [ACE2: B0AT1] heterodimer pair constitutes a physiological “functional unit.” This was confirmed experimentally by employing purified lyophilized enterocyte brush border membrane vesicles that were exposed to increasing doses of high-energy electron radiation from a 16 MeV linear accelerator. Based on established target theory, the results indicated the presence of Na+-dependent neutral amino acid influx transport activity functional unit with target size mw = 183.7 ± 16.8 kDa in situ in intact apical membranes. Each thermodynamically stabilized [ACE2: B0AT1] heterodimer functional unit manifests the transport activity within the whole ∼345 kDa [ACE2: B0AT1]2 dimer-of-heterodimers quaternary structural complex. The results are consistent with our prior molecular docking modeling and gut-lung axis approaches to understanding COVID-19. These findings advance the understanding of the physiology of B0AT1 interaction with ACE2 in the gut, and thereby potentially contribute to translational developments designed to treat or mitigate COVID-19 variant outbreaks and/or GI symptom persistence in long-haul Post-Acute Sequelae of SARS-CoV-2 (PASC).

Stratigraphy and palaeoenvironments of the Red Crag and Norwich Crag formations between Aldeburgh and Sizewell, Suffolk, England

1988 ◽  
Vol 322 (1210) ◽  
pp. 221-272 ◽  
Keyword(s):  
Amino Acid ◽  
High Energy ◽  
Coarse Grained ◽  
Core Material ◽  
Stage System ◽  
Pollen And Spores ◽  
Shallow Marine Sediments ◽  
Amino Acid Chronology ◽  
Tectonic Origin ◽  
Borehole Core

This study uses a variety of criteria to examine short-range correlation within the Crag deposits in order to assess the validity of longer-range correlations within the British Pleistocene stage system. To this end, six rotary cored boreholes spaced at 0.5-1.0 km intervals were drilled along a north-south-aligned traverse between Aldeburgh and Sizewell, Suffolk. These show that the thick Red/Norwich Crag sequence is confined to a deep, sharply bounded basin, which is of probable erosional rather than tectonic origin. The undisturbed borehole core material enabled an assessment of the limits of stratigraphic resolution within these dominantly high-energy, shallow marine sediments to be made. Subdivision of the sequence was done on the basis of lithostratigraphical and biostratigraphical (foraminifera, pollen and spores, dinoflagellate cysts, and molluscs) criteria; chronostratigraphical methods (palaeomagnetism and amino acid chronology) were also applied. The various subdivisions indicated by each of these disciplines were in large part consistent, demonstrating that valid stratigraphic units had been identified. Only amino acid chronology did not indicate any obvious subdivision of the sequence. Three lithostratigraphical units were recognized within the thick Crag sequence. The lowest unit (AS-Lith 1) consists of coarse shelly sands interbedded with thinly laminated muds and fine sands. The middle unit (AS-Lith 2) consists of fine- to coarse-grained shelly sands arranged in two coarsening-upwards cycles. Units AS-Lith 1 and AS-Lith 2 are correlated on a lithostratigraphical basis with the Red Crag Formation of the adjacent Aldeburgh-Orford area to the south and are named the Sizewell Member and the Thorpeness Member respectively. The uppermost unit (AS-Lith 3) comprises fine- to medium-grained, well-sorted sands; it correlates with the Chillesford Sand Member of the Norwich Crag Formation of the adjacent Aldeburgh-Orford area. The Sizewell Member of the Red Crag Formation is normally magnetized and palaeontologically distinctive. The pollen, foraminifera and dinoflagellate assemblages firmly establish it as Pre-Ludhamian in age, and probably equivalent to an interval within the Reuverian C to Praetiglian Stages of the Netherlands. The Thorpeness Member of the Red Crag Formation is less easy to place within the British Pleistocene stage system. It is reverse magnetized, at least in part, and foraminifera assemblages suggest possible correlation with the Ludhamian Stage. No identifiable pollen or dinoflagellate assemblages were obtained. The Chillesford Sand Member of the Norwich Crag Formation is largely unfossiliferous but the borehole material has yielded a single pollen spectrum that suggests correlation with the Bramertonian Stage.

Plasma essential amino acids concentrations of early lactating Holstein dairy cows fed diet containing raw or roasted Iranian soybean

2007 ◽  
Vol 2007 ◽  
pp. 182-182
Author(s):  
Forouzan Tabatabaie ◽  
Hassan Fathi ◽  
Mohsen Danesh
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dairy Cows ◽  
Physical Method ◽  
Essential Amino Acids ◽  
High Energy ◽  
Early Lactation ◽  
High Amino Acid ◽  
Amino Acid Requirements ◽  
Physical Treatments

Whole soybean has 40-42 percent CP and used as high energy-protein supplement for early lactation dairy cows. However, the protein is highly degradable, so small amounts of amino acids can be reached to small intestine to meet high amino acid requirements of early lactating cows. Therefore, various chemical and physical treatments have been suggested to decrease ruminal protein degradability of soybeans. The practical use and application of any one method to lower ruminal feed degradability is dependent not only on its efficacy but also on its cost effectiveness, safety and ease of application. For these reasons, heat treatment is the most commonly used physical method (Plegge et al., 1985). The purpose of this study was to determine how roasting of soybeans affect plasma essential amino acid concentrations in early lactation cows.

Effect of amino group protonation on the carboxyl group in aqueous glycine observed by O 1s X-ray emission spectroscopy

2018 ◽  
Vol 20 (36) ◽  
pp. 23214-23221 ◽  
Author(s):  
Y. Horikawa ◽  
T. Tokushima ◽  
O. Takahashi ◽  
Y. Harada ◽  
A. Hiraya ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Amino Acid ◽  
Electronic Structures ◽  
Emission Spectroscopy ◽  
Selective Excitation ◽  
Carboxyl Group ◽  
Amino Group ◽  
X Ray ◽  
Excitation Conditions ◽  
Amino Acid Glycine

The valence electronic structures of the amino acid glycine in aqueous solution were investigated in detail through X-ray emission spectroscopy at O 1s excitation under selective excitation conditions of the CO site in the carboxyl group.

scholarly journals Radiocarbon Dating of Pleistocene Bone: Toward Criteria for the Selection of Samples

Radiocarbon ◽  
1980 ◽  
Vol 22 (3) ◽  
pp. 969-979 ◽  
Author(s):  
R E Taylor
Keyword(s):  
Amino Acid ◽  
Stable Isotope ◽  
Amino Acid Composition ◽  
Radiocarbon Dating ◽  
Detection System ◽  
High Energy ◽  
Mass Spectrometric ◽  
Stable Isotope Ratios ◽  
Sources Of Information ◽  
Selection Of

Amino acid composition data and stable isotope ratios (14N, D, and13C) are being evaluated as sources of information to indicate the presence of non-indigenous organics in bone samples intended for 14C analyses. The study is being conducted in the context of the planned measurement of Pleistocene bone samples by a high energy mass spectrometric 14C detection system.

scholarly journals Achiral amino acid glycine acts as an origin of homochirality in asymmetric autocatalysis

2019 ◽  
Vol 17 (17) ◽  
pp. 4200-4203 ◽  
Author(s):  
Arimasa Matsumoto ◽  
Hanae Ozaki ◽  
Sumeru Tsuchiya ◽  
Toru Asahi ◽  
Meir Lahav ◽  
...  
Keyword(s):  
Amino Acid ◽  
Asymmetric Autocatalysis ◽  
Origin Of Chirality ◽  
Origin Of Homochirality ◽  
Amino Acid Glycine

Achiral glycine acts as the origin of chirality in conjunction with asymmetric autocatalysis to afford highly enantioenriched compound.

Effect of compatible solutes and diluent composition on the post-thaw motility of ram sperm

1998 ◽  
Vol 10 (4) ◽  
pp. 347 ◽  
Author(s):  
L. G. Sánchez-Partida ◽  
B. P. Setchell ◽  
W. M. C. Maxwell
Keyword(s):  
Amino Acid ◽  
Glycine Betaine ◽  
Detrimental Effect ◽  
Compatible Solutes ◽  
Egg Yolk ◽  
Motile Sperm ◽  
Motility Of Sperm ◽  
Ram Sperm ◽  
Amino Acid Glycine

The effect of the compatible solutes proline, glycine betaine and trehalose in Tris-based diluents at varying pH, concentrations of egg yolk or glycerol on the post-thaw motility characteristics and fertility of ram sperm was examined. In addition, the amino acid glycine was compared with proline, glycine betaine and a standard Tris-based diluent. Post-thaw motility was assessed using a Hamilton–Thorn motility analyser. In the presence of glycerol and egg yolk, proline and glycine betaine improved the post-thaw motility characteristics of ram sperm. Regardless of the pH of the diluent at which semen was frozen, the percentage of motile sperm was higher when frozen in the presence of proline or glycine betaine than in their absence, whereas proline and glycine betaine only improved the progressive and rapid percentages of sperm for semen frozen in diluents at pH lower than 7.0. When semen was frozen in the absence of egg yolk or glycerol all the motility characteristics were reduced. Increasing the concentration of egg yolk in the diluent from 5% to 10, 15 or 20% had no effect on the post-thaw motility of sperm. The addition of 27 mM of proline or glycine betaine to the diluent also improved post-thaw motility. However, at a concentration of 81 mM, proline and glycine betaine had a detrimental effect on the percentage of motile sperm. Trehalose had no effect on the motility of sperm frozen in glycerol-containing diluents, but motility was lower after cryopreservation in glycine than in Tris-, proline- or glycine betaine-based diluents. There were no differences in the fertility of sperm frozen in Tris-, proline or glycine betaine diluents after cervical or laparo-scopic insemination of ewes.

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