A Way to Probe the Microenvironment of Free Sulfhydryls in Intact Proteins with a Series of Monofunctional Organic Mercurials

2012 ◽  
Vol 18 (44) ◽  
pp. 13989-13993 ◽  
Author(s):  
Ming Xu ◽  
Limin Yang ◽  
Qiuquan Wang
Keyword(s):  
Intact Proteins ◽  
Organic Mercurials

scholarly journals Identification and Characterization of Novel Antioxidant Protein Hydrolysates from Kiwicha (Amaranthus caudatus L.)

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 645
Author(s):  
Sergio Montserrat-de la Paz ◽  
Alicia Martinez-Lopez ◽  
Alvaro Villanueva-Lazo ◽  
Justo Pedroche ◽  
Francisco Millan ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Reducing Power ◽  
Protein Isolate ◽  
Protein Hydrolysates ◽  
Antioxidant Peptides ◽  
Amaranthus Caudatus ◽  
Intact Proteins ◽  
Identification And Characterization

Kiwicha (Amaranthus caudatus) is considered one of the few multipurpose pseudocereals for its potential use not only as a source of nutrients and fiber but also for its bioactive compounds. In recent years, antioxidant peptides are commonly used as functional ingredient of food. Herein, a kiwicha protein isolate (KPI), obtained from kiwicha defatted flour (KDF), was hydrolyzed by Bioprotease LA 660, a food-grade endoprotease, under specific conditions. The resulting kiwicha protein hydrolysates (KPHs) were chemically characterized and their digestibility and antioxidant capacity were evaluated by in vitro cell-free experiments owing to their measure of capacity to sequester DPPH free radical and reducing power. KPHs showed higher digestibility and antioxidant capacity than intact proteins into KPI. Therefore, the results shown in this study indicate that KPHs could serve as an adequate source of antioxidant peptides, representing an effective alternative to the generation of functional food.

scholarly journals Novel sheathless CE-MS interface as an original and powerful infusion platform for nanoESI study: from intact proteins to high molecular mass noncovalent complexes

2013 ◽  
Vol 406 (4) ◽  
pp. 1029-1038 ◽  
Author(s):  
Rabah Gahoual ◽  
Jean-Marc Busnel ◽  
Philippe Wolff ◽  
Yannis Nicolas François ◽  
Emmanuelle Leize-Wagner
Keyword(s):  
Molecular Mass ◽  
High Molecular Mass ◽  
Noncovalent Complexes ◽  
Intact Proteins

The effect of sample salt additives on capillary electrophoresis analysis of intact proteins using surface modified capillaries

2009 ◽  
Vol 1216 (17) ◽  
pp. 3613-3620 ◽  
Author(s):  
Anisa Elhamili ◽  
Magnus Wetterhall ◽  
Angel Puerta ◽  
Douglas Westerlund ◽  
Jonas Bergquist
Keyword(s):  
Capillary Electrophoresis ◽  
Intact Proteins ◽  
Surface Modified

A setup for simultaneous measurement of infrared spectra and light scattering signals: Watching amyloid fibrils grow from intact proteins

2014 ◽  
Vol 85 (8) ◽  
pp. 084302 ◽  
Author(s):  
Yang Li ◽  
Jürgen Maurer ◽  
Andreas Roth ◽  
Vitali Vogel ◽  
Ernst Winter ◽  
...  
Keyword(s):  
Light Scattering ◽  
Infrared Spectra ◽  
Simultaneous Measurement ◽  
Amyloid Fibrils ◽  
Intact Proteins

Direct fluorimetric assay for proteolytic activity against intact proteins

1975 ◽  
Vol 64 (2) ◽  
pp. 556-566 ◽  
Author(s):  
Patricia W. Spencer ◽  
John S. Titus ◽  
Richard D. Spencer
Keyword(s):  
Proteolytic Activity ◽  
Intact Proteins ◽  
Fluorimetric Assay
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