Highly Fluorescent Conjugated Pyrenes in Nucleic Acid Probes: (Phenylethynyl)pyrenecarbonyl-Functionalized Locked Nucleic Acids

2008 ◽  
Vol 14 (35) ◽  
pp. 11010-11026 ◽  
Author(s):  
Irina V. Astakhova ◽  
Vladimir A. Korshun ◽  
Jesper Wengel
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Probes ◽  
Locked Nucleic Acids

scholarly journals Bridged Nucleic Acids Reloaded

2019 ◽  
Author(s):  
Alfonso Soler-Bistué ◽  
Angeles Zorreguieta ◽  
Marcelo E. Tolmasky
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
First Generation ◽  
Second Generation ◽  
Nucleotide Analogs ◽  
Great Hope ◽  
Locked Nucleic Acids ◽  
Near Future

Oligonucleotides are key compounds widely used for research, diagnostics, and therapeutics. The rapid increase in oligonucleotide-based applications, together with the progress in nucleic acids research, led to the design of nucleotide analogs that when being part of these oligomers enhance their efficiency, bioavailability, or stability. One of the most useful nucleotide analogs are the first-generation bridge nucleic acids (BNA), also known as locked nucleic acids (LNA), which were used in combination with ribonucleotides, deoxyribonucleotides, or other analogs to construct oligomers with diverse applications. However, there is still room to improve their efficiency, bioavailability, stability, and, importantly, toxicity. A second generation BNA, BNANC (2'-O,4'-aminoethylene bridged nucleic acid), has been recently made available. Oligomers containing these analogs not only showed less toxicity when compared to LNA-containing compounds but in some cases also exhibited higher specificity. Although there are still few applications where BNANC-containing compounds were researched, the results are very promising warranting more efforts in incorporating these analogs for other applications. Furthermore, newer BNA compounds will be introduced in the near future offering great hope to oligonucleotide-based fields of research and applications.

scholarly journals Bridged Nucleic Acids Reloaded

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2297 ◽  
Author(s):  
Alfonso Soler-Bistué ◽  
Angeles Zorreguieta ◽  
Marcelo E. Tolmasky
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
First Generation ◽  
Second Generation ◽  
Nucleotide Analogs ◽  
Great Hope ◽  
Locked Nucleic Acids ◽  
Near Future

Oligonucleotides are key compounds widely used for research, diagnostics, and therapeutics. The rapid increase in oligonucleotide-based applications, together with the progress in nucleic acids research, has led to the design of nucleotide analogs that, when part of these oligomers, enhance their efficiency, bioavailability, or stability. One of the most useful nucleotide analogs is the first-generation bridged nucleic acids (BNA), also known as locked nucleic acids (LNA), which were used in combination with ribonucleotides, deoxyribonucleotides, or other analogs to construct oligomers with diverse applications. However, there is still room to improve their efficiency, bioavailability, stability, and, importantly, toxicity. A second-generation BNA, BNANC (2′-O,4′-aminoethylene bridged nucleic acid), has been recently made available. Oligomers containing these analogs not only showed less toxicity when compared to LNA-containing compounds but, in some cases, also exhibited higher specificity. Although there are still few applications where BNANC-containing compounds have been researched, the promising results warrant more effort in incorporating these analogs for other applications. Furthermore, newer BNA compounds will be introduced in the near future, offering great hope to oligonucleotide-based fields of research and applications.

scholarly journals Strand displacement and duplex invasion into double-stranded DNA by pyrrolidinyl peptide nucleic acids

2015 ◽  
Vol 13 (35) ◽  
pp. 9223-9230 ◽  
Author(s):  
Peggy R. Bohländer ◽  
Tirayut Vilaivan ◽  
Hans-Achim Wagenknecht
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acids ◽  
Dna Duplexes ◽  
Strand Displacement ◽  
Nucleic Acid Probes ◽  
Double Stranded Dna

Strand displacement and duplex invasion of DNA duplexes by pyrrolidinyl peptide nucleic acid are demonstrated using the concept of wavelength-shifting nucleic acid probes.

scholarly journals Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids

2019 ◽  
Vol 49 (1) ◽  
Author(s):  
Akimitsu Okamoto
Keyword(s):  
Gene Expression ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Fluorescence Imaging ◽  
Cell Function ◽  
Microscopic Analysis ◽  
Next Generation ◽  
Nucleic Acid Probes ◽  
Specific Fluorescence ◽  
Methylated Dna

AbstractFluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated DNA using fluorescent nucleic acid probes. A couple of functional fluorescent nucleic acid probes developed by our laboratory are introduced and the examples of their application to fluorescence imaging of intracellular nucleic acids are described.

LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition

1998 ◽  
pp. 455-456 ◽  
Author(s):  
Sanjay K. Singh ◽  
Alexei A. Koshkin ◽  
Jesper Wengel ◽  
Poul Nielsen
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
High Affinity ◽  
Locked Nucleic Acids

LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition

Tetrahedron ◽  
1998 ◽  
Vol 54 (14) ◽  
pp. 3607-3630 ◽  
Author(s):  
Alexei A. Koshkin ◽  
Sanjay K. Singh ◽  
Poul Nielsen ◽  
Vivek K. Rajwanshi ◽  
Ravindra Kumar ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Locked Nucleic Acids

ChemInform Abstract: LNA (Locked Nucleic Acids): Synthesis and High-Affinity Nucleic Acid Recognition.

ChemInform ◽  
2010 ◽  
Vol 29 (26) ◽  
pp. no-no
Author(s):  
S. K. SINGH ◽  
P. NIELSEN ◽  
A. A. KOSHKIN ◽  
J. WENGEL
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
High Affinity ◽  
Locked Nucleic Acids
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