Novel Probes Showing Specific Fluorescence Enhancement on Binding to a Hexahistidine Tag

2008 ◽  
Vol 14 (26) ◽  
pp. 8004-8012 ◽  
Author(s):  
Mie Kamoto ◽  
Naoki Umezawa ◽  
Nobuki Kato ◽  
Tsunehiko Higuchi
Keyword(s):  
Fluorescence Enhancement ◽  
Specific Fluorescence ◽  
Hexahistidine Tag

TQPHEN (N,N,N′,N′-tetrakis(2-quinolylmethyl)-1,2-phenylenediamine) derivatives as highly selective fluorescent probes for Cd2+

2015 ◽  
Vol 44 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Yuji Mikata ◽  
Asako Kizu ◽  
Hideo Konno
Keyword(s):  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Specific Fluorescence

The tetrakisquinoline derivatives with a 1,2-phenylenediamine (PHEN) scaffold exhibit a Cd2+-specific fluorescence enhancement, in contrast to the Zn2+-specific trans-1,2-diaminocyclohexane (DACH) counterpart.

Methoxy-substituted tetrakisquinoline analogs of EGTA and BAPTA for fluorescence detection of Cd2+

2019 ◽  
Vol 48 (12) ◽  
pp. 3840-3852 ◽  
Author(s):  
Yuji Mikata ◽  
Minori Kaneda ◽  
Hideo Konno ◽  
Arimasa Matsumoto ◽  
Shin-ichiro Sato ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Enhancement ◽  
Tetraacetic Acid ◽  
Specific Fluorescence ◽  
Octadentate Ligand ◽  
Acid Structure

A 5,6,7-trimethoxyquinoline-based octadentate ligand with a BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid) structure exhibits Cd2+-specific fluorescence enhancement.

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

The Difference in Sensing Properties Towards Anions between Two Pyridinium Amide-Based Receptors with a Subtle Change of H-Bond Position

2020 ◽  
Vol 17 (6) ◽  
pp. 472-478
Author(s):  
Wei-tao Gong ◽  
Wei-dong Qu ◽  
Guiling Ning
Keyword(s):  
Fluorescence Enhancement ◽  
Small Difference ◽  
Fluorescence Emission ◽  
Subtle Change ◽  
Sensing Properties ◽  
Naked Eye ◽  
Recognition Ability ◽  
The Difference ◽  
L1 And L2

Two pyridinium amide-based receptors L1 and L2 with a small difference of H-bond position of the amide have been synthesized and characterized. Interestingly, they exhibited a huge difference in sensing towards AcO- and H2PO4 -, respectively. Receptor L1 was found to be ‘naked-eye’ selective for AcO- anions, while receptor L2 showed clear fluorescence enhancement selective to H2PO4 - anion. The recognition ability has been established by fluorescence emission, UV-vis spectra, and 1HNMR titration.

Semiautomated microfluorometric instrument and reagent system for monitoring disease-related immunosuppression.

1982 ◽  
Vol 28 (9) ◽  
pp. 1887-1893 ◽  
Author(s):  
D F Ranney ◽  
A J Quattrone
Keyword(s):  
Immune Response ◽  
Dna Content ◽  
Peripheral Blood ◽  
Fluorescence Enhancement ◽  
Photon Counting ◽  
Peripheral Blood Lymphocytes ◽  
Response Modulation ◽  
Blood Lymphocytes ◽  
Complex Test ◽  
Immune Response Modulation

Abstract Several common metabolites and drugs in the serum in of patients with inflammatory, infectious, autoimmune, immunodeficient, neoplastic, and toxicant-induced diseases can produce artifactual suppression of the [methyl-3H]-thymidine assay, which is widely used to evaluate lymphocyte responsiveness. We have developed a sensitive, semiautomated, fluorescence-enhancement assay in which true immunosuppressors are measured in the presence of absence of such interfering substances. Peripheral blood lymphocytes are activated with mitogens in standard microtiter culture trays. Changes in lymphocyte DNA content are quantified with a reagent formulation containing mithramycin, the fluorescence of which is enhanced on binding to DNA in the presence of MgCl2. We solubilize cells within the intact microtiter tray by using an automated, inverted "Array Sonicator," and measure fluorescence with an automated, photon-counting fluorometer. With this system, immune response modulation can be accurately assessed in the presence of patients' sera and other complex test substances (e.g., supernates from hybridomas, fermentation vats, viral preparations, and macrophage cultures.

Controlled Fluorescence Enhancement of DNA-Binding Dye Through Chain Length Match between Oligoguanine and TOTO

2021 ◽  
Vol 125 (2) ◽  
pp. 518-527
Author(s):  
Fangjia Fu ◽  
Kang Liao ◽  
Ziteng Liu ◽  
Daocheng Hong ◽  
Haitang Yang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Chain Length ◽  
Fluorescence Enhancement

Sensitive and specific detection of peroxynitrite and in-vivo imaging of inflammation by a "simple" AIE bioprobe

2021 ◽  
Author(s):  
Huilin Xie ◽  
Jingtian Zhang ◽  
Chao Chen ◽  
Feiyi Sun ◽  
Haixiang Liu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescence Enhancement ◽  
Specific Detection ◽  
Aggregation Induced Emission

A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO−) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage...

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