Ethylene Biosynthesis by 1-Aminocyclopropane-1-Carboxylic Acid Oxidase: A DFT Study

2006 ◽  
Vol 12 (34) ◽  
pp. 8835-8846 ◽  
Author(s):  
Arianna Bassan ◽  
Tomasz Borowski ◽  
Christopher J. Schofield ◽  
Per E. M. Siegbahn
Keyword(s):  
Carboxylic Acid ◽  
Ethylene Biosynthesis ◽  
Dft Study ◽  
Acid Oxidase

scholarly journals Expression Analysis of 1-aminocyclopropane-1-carboxylic Acid Oxidase Genes in Chitosan-Coated Banana

2018 ◽  
Vol 25 (1) ◽  
pp. 18
Author(s):  
Kana Yamamoto ◽  
Annisa Amalia ◽  
Sastia P. Putri ◽  
Eiichiro Fukusaki ◽  
Fenny M. Dwivany
Keyword(s):  
Carboxylic Acid ◽  
Expression Patterns ◽  
Ethylene Biosynthesis ◽  
The Other ◽  
Acid Oxidase ◽  
Biosynthesis Pathway ◽  
Chitosan Coating ◽  
Climacteric Fruit ◽  
Similar Expression Pattern ◽  
Fruit Shelf Life

Banana is a climacteric fruit in which ethylene plays an important role in the regulation of the ripening process. Though it is the most produced fruit in Indonesia, the current post-harvest technologies for exporting this fruit are not economically friendly. Chitosan is one of economical biopolymer for edible coating which can extend fruit shelf-life. However, little study focused on the effect of chitosan coating has been done on gene expression level. In this study, the expression levels of several 1-aminocyclopropan-1-carboxylic acid oxidase (ACO) genes, which is an enzyme to convert 1-aminocyclopropan-1-carboxylic acid to ethylene in banana were analyzed on day 0, 1, 3, 5, 7, and 9 after ethylene treatment. As a result, one gene (ID: Ma01_t11540.1) had a similar expression pattern in both control and chitosan-coated bananas while the other genes (ID: Ma03_t02700.1, Ma05_t09360.1, Ma06_t02600.1, Ma10_t01130.1) showed different expression patterns. Among these genes, two genes (ID: Ma05_t09360.1, Ma10_t01130.1) were expressed higher than the other genes and the peak was observed on day 3. It was indicated that chitosan coating might activate the ethylene biosynthesis pathway in banana while it delayed fruit ripening.

scholarly journals The NAM/ATAF1/2/CUC2 transcription factor PpNAC.A59 enhances PpERF.A16 expression to promote ethylene biosynthesis during peach fruit ripening

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Zhi-Hua Guo ◽  
You-Jia Zhang ◽  
Jia-Long Yao ◽  
Zhi-Hua Xie ◽  
Yu-Yan Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Carboxylic Acid ◽  
Fruit Ripening ◽  
Genetic Network ◽  
Ethylene Biosynthesis ◽  
Ethylene Response Factor ◽  
Acid Oxidase ◽  
Peach Fruit ◽  
Climacteric Fruit ◽  
Exogenous Ethylene

AbstractPeach is a typical climacteric fruit that releases ethylene during fruit ripening. Several studies have been conducted on the transcriptional regulation of ethylene biosynthesis in peach fruit. Herein, an ethylene response factor, PpERF.A16, which was induced by exogenous ethylene, could enhance ethylene biosynthesis by directly inducing the expression of 1-aminocyclopropane-1-carboxylic acid synthase (PpACS1) and 1-aminocyclopropane-1-carboxylic acid oxidase (PpACO1) genes. Moreover, the NAM/ATAF1/2/CUC2 (NAC) transcription factor (TF) PpNAC.A59 was coexpressed with PpERF.A16 in all tested peach cultivars. Interestingly, PpNAC.A59 can directly interact with the promoter of PpERF.A16 to induce its expression but not enhance LUC activity driven by any promoter of PpACS1 or PpACO1. Thus, PpNAC.A59 can indirectly mediate ethylene biosynthesis via the NAC-ERF signaling cascade to induce the expression of both PpACS1 and PpACO1. These results enrich the genetic network of fruit ripening in peach and provide new insight into the ripening mechanism of other perennial fruits.

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