Metal-Binding Properties of the Peptide APP170-188: A Model of the ZnII-Binding Site of Amyloid Precursor Protein (APP)

2005 ◽  
Vol 11 (3) ◽  
pp. 903-909 ◽  
Author(s):  
Eliza-Diana Ciuculescu ◽  
Yasmina Mekmouche ◽  
Peter Faller
Keyword(s):  
Amyloid Precursor Protein ◽  
Binding Site ◽  
Metal Binding ◽  
Precursor Protein ◽  
Binding Properties

scholarly journals Evaluation of Cu(i) binding to the E2 domain of the amyloid precursor protein – a lesson in quantification of metal binding to proteinsvialigand competition

Metallomics ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 108-119 ◽  
Author(s):  
Tessa R. Young ◽  
Anthony G. Wedd ◽  
Zhiguang Xiao
Keyword(s):  
Systematic Review ◽  
Amyloid Precursor Protein ◽  
Metal Binding ◽  
Protein A ◽  
Ternary Complexes ◽  
Precursor Protein ◽  
Physiological Functions ◽  
Protein Affinity ◽  
General Conditions

APP E2 forms stable Cu(i)-mediated ternary complexes with several competing ligands, but not upon addition of heparin. This allows a systematic review of general conditions for quantification of metal–protein affinity and new insights into the physiological functions of APP.

Identification of a novel high affinity copper binding site in the APP(145–155) fragment of amyloid precursor protein

2004 ◽  
pp. 16-22 ◽  
Author(s):  
Daniela Valensin ◽  
Francesca Maria Mancini ◽  
Marek Łuczkowski ◽  
Anna Janicka ◽  
Kornelia Wiśniewska ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Binding Site ◽  
Precursor Protein ◽  
Copper Binding ◽  
High Affinity

scholarly journals Unravelling the Structure of the Tetrahedral Metal-Binding Site in METP3 through an Experimental and Computational Approach

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5221
Author(s):  
Salvatore La Gatta ◽  
Linda Leone ◽  
Ornella Maglio ◽  
Maria De Fenza ◽  
Flavia Nastri ◽  
...  
Keyword(s):  
Metal Ions ◽  
Binding Site ◽  
Metal Binding ◽  
Binding Sites ◽  
Metal Ion ◽  
Structural Determinants ◽  
Binding Properties ◽  
Tetrahedral Geometry ◽  
Design Synthesis ◽  
Metal Binding Sites

Understanding the structural determinants for metal ion coordination in metalloproteins is a fundamental issue for designing metal binding sites with predetermined geometry and activity. In order to achieve this, we report in this paper the design, synthesis and metal binding properties of METP3, a homodimer made up of a small peptide, which self assembles in the presence of tetrahedrally coordinating metal ions. METP3 was obtained through a redesign approach, starting from the previously developed METP molecule. The undecapeptide sequence of METP, which dimerizes to house a Cys4 tetrahedral binding site, was redesigned in order to accommodate a Cys2His2 site. The binding properties of METP3 were determined toward different metal ions. Successful assembly of METP3 with Co(II), Zn(II) and Cd(II), in the expected 2:1 stoichiometry and tetrahedral geometry was proven by UV-visible spectroscopy. CD measurements on both the free and metal-bound forms revealed that the metal coordination drives the peptide chain to fold into a turned conformation. Finally, NMR data of the Zn(II)-METP3 complex, together with a retrostructural analysis of the Cys-X-X-His motif in metalloproteins, allowed us to define the model structure. All the results establish the suitability of the short METP sequence for accommodating tetrahedral metal binding sites, regardless of the first coordination ligands.

scholarly journals Identification and Characterization of a κB/Rel Binding Site in the Regulatory Region of the Amyloid Precursor Protein Gene

1995 ◽  
Vol 270 (45) ◽  
pp. 26774-26777 ◽  
Author(s):  
Mariagrazia Grilli ◽  
Marina Ribola ◽  
Antonella Alberici ◽  
Alessandra Valerio ◽  
Maurizio Memo ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Binding Site ◽  
Protein Gene ◽  
Regulatory Region ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Gene ◽  
Identification And Characterization

scholarly journals Effect of Common Buffers and Heterocyclic Ligands on the Binding of Cu(II) at the Multimetal Binding Site in Human Serum Albumin

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Magdalena Sokołowska ◽  
Krystyna Pawlas ◽  
Wojciech Bal
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Site ◽  
Metal Binding ◽  
Visible Range ◽  
Heterocyclic Ligands ◽  
Binding Properties ◽  
Spectral Changes ◽  
Conformational Effects

Visible-range circular dichroism titrations were used to study Cu(II) binding properties of Multimetal Binding Site (MBS) of Human Serum Albumin (HSA). The formation of ternary MBS-Cu(II)-Buffer complexes at pH 7.4 was positively verified for sodium phosphate, Tris, and Hepes, the three most common biochemical buffers. The phosphate > Hepes > Tris order of affinities, together with strong spectral changes induced specifically by Tris, indicates the presence of both Buffer-Cu(II) and Buffer-HSA interactions. All complexes are strong enough to yield a nearly 100% ternary complex formation in 0.5 mM HSA dissolved in 100 mM solutions of respective buffers. The effects of warfarin and ibuprofen, specific ligands of hydrophobic pockets I and II in HSA on the Cu(II) binding to MBS were also investigated. The effects of ibuprofen were negligible, but warfarin diminished the MBS affinity for Cu(II) by a factor of 20, as a result of indirect conformational effects. These results indicate that metal binding properties of MBS can be modulated directly and indirectly by small molecules.

Bacterial and cell-free production of APP671-726 containing amyloid precursor protein transmembrane and metal-binding domains

2013 ◽  
Vol 78 (11) ◽  
pp. 1263-1271 ◽  
Author(s):  
O. V. Bocharova ◽  
A. S. Urban ◽  
K. D. Nadezhdin ◽  
E. V. Bocharov ◽  
A. S. Arseniev
Keyword(s):  
Amyloid Precursor Protein ◽  
Metal Binding ◽  
Precursor Protein ◽  
Binding Domains

scholarly journals Metal Binding Dictates Conformation and Function of the Amyloid Precursor Protein (APP) E2 Domain

2012 ◽  
Vol 416 (3) ◽  
pp. 438-452 ◽  
Author(s):  
Sven O. Dahms ◽  
Ina Könnig ◽  
Dirk Roeser ◽  
Karl-Heinz Gührs ◽  
Magnus C. Mayer ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Metal Binding ◽  
Precursor Protein ◽  
And Function
Sign in / Sign up

Export Citation Format

Share Document