Biodiesel Production: Effects of the Feeding Process on Biomass and Lipid Productivity

2016 ◽  
Vol 39 (8) ◽  
pp. 1468-1474 ◽  
Author(s):  
Seyed M. Fatmei ◽  
Rasoul Khalilzadeh ◽  
Hamid R. Azizi ◽  
Saba Alipouryan
Keyword(s):  
Lipid Productivity ◽  
Biodiesel Production ◽  
Feeding Process ◽  
Production Effects

Evaluation of microalgal strains and microalgal consortium for higher lipid productivity and rich fatty acid profile towards sustainable biodiesel production

2021 ◽  
pp. 125524
Author(s):  
Chitirai Arutselvan ◽  
Ganeshan Narchonai ◽  
Arivalagan Pugazhendhi ◽  
Felix Lewis Oscar ◽  
Nooruddin Thajuddin
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Lipid Productivity ◽  
Biodiesel Production

Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production

2014 ◽  
Vol 153 ◽  
pp. 230-235 ◽  
Author(s):  
Ayumi Tanimura ◽  
Masako Takashima ◽  
Takashi Sugita ◽  
Rikiya Endoh ◽  
Minako Kikukawa ◽  
...  
Keyword(s):  
Lipid Productivity ◽  
Biodiesel Production ◽  
Oleaginous Yeasts ◽  
High Lipid ◽  
Selection Of

scholarly journals Coupling of Nutrient Removal and Biodiesel Production By Asterarcys quadricellulare Microalga Grown in Municipal Wastewater

2021 ◽  
Author(s):  
Hanaa Morsi ◽  
Hamed Eladel ◽  
Ayah Maher
Keyword(s):  
Municipal Wastewater ◽  
Basal Medium ◽  
Biomass Productivity ◽  
International Standards ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Fatty Acids Profile ◽  
High Lipid ◽  
Biodiesel Feedstock ◽  
Profile Estimation

Abstract The present study focused on the feasibility of using municipal wastewater (WW) as culture medium for cultivation of microalgae. The study aimed to assess the efficiency of microalgae in nutrients removing capacity from wastewater and its biomass and lipid productivity for using as biodiesel feedstock. Based on that, the green microalga Asterarcys quadricellulare was isolated and grown for 24 days in Bold’s Basal Medium as a control and at different concentration of secondary treated municipal wastewater (WW) diluted with distilled water (25%, 50%, 75% and 100%WW). Results of 75%WW treatment recorded 96.6%, 98.4%, and 89.9% removal efficiency for, nitrate, ammonia and total phosphorus, respectively. Also, it revealed high biomass productivity and biomass content, where it recorded 69.0 mgL-1 day-1, and 1.44 g/L, respectively. Likewise, high lipid productivity 17.2 mg L−1 day−1 and 360.6 mg/L lipid content. Consequently, Asterarcys quadricellulare fatty acids profile estimation revealed an increase in Oleic, Palmitic and Linoleic acids levels. Most properties of biodiesel derived from the studied microalga meet with values established by the ASTM D6751 and EN 14214 biodiesel standards. According to this analysis, A. quadricellulare microalga could be used for wastewater bioremediation and biomass production with a suitable content of lipids proper as biodiesel feedstock. The predictive biodiesel properties pointed that it has a good quality compared with international standards.

scholarly journals Assessment of microalgal ACCase and rbcl gene expression as a function of nutrient and metal stress

2017 ◽  
Author(s):  
◽  
Poonam Singh
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Biomass Productivity ◽  
Photosynthetic Performance ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Rbcl Gene ◽  
Limited Condition ◽  
Bg11 Medium ◽  
Microalgal Biodiesel

Microalgae are considered to be a potential feedstock for biodiesel production. However, the main concern with regard to the large scale microalgal biodiesel production process is its competence and economic viability. The commercial realization of microalgal biodiesel production requires substantial impetus towards development of efficient strategies to improve lipid yields upstream. Nitrogen (N) and phosphorus (P) stress during cultivation are the widely used lipid accumulation strategies for microalgae. However, these individual nutrient stress strategies are associated with compromised biomass productivity which hampers overall lipid productivity. Lipid enhancement strategies based on light, temperature and CO2 are associated with technological barriers for scale up and incur additional cost. Thus, the main aim of this study was to develop an integrated, easily applicable and scalable lipid enhancement strategy based on nutrients and metals such as N, P, iron (Fe), magnesium (Mg), calcium (Ca) and EDTA stress for selected indigenous microalgal strains. The effect of metal concentrations individually and in combination on microalgal lipids and biomass production is a scarcely exploited area. In this study, a novel approach involving individual as well as combined metals and EDTA stress under N and P limited conditions for lipid enhancement in microalgae was investigated. Microalgal growth physiology, photosynthetic performance, biochemical composition (lipid, carbohydrate and protein) and expression of selected key genes involved in photosynthesis (rbcL) and fatty acid biosynthesis (accD) were studied both under selected individual and combined stress conditions. Out of seven microalgal isolates obtained during the initial isolation and screening process, two strains were selected for lipid enhancement study based on their growth rates, biomass yields, lipid content and lipid productivities. The strains were later identified as Acutodesmus obliquus and Chlorella sorokiniana based on both morphological characteristics and phylogenetical analysis. The selected strains were thereafter subjected to different cultivation conditions involving varying metal, EDTA and nutrient stress conditions. A significant increase in lipid productivity was observed when the concentrations of Fe, Mg and EDTA were increased and Ca was decreased to degree in the N and P stress BG11 medium. For A. obliquus, a highest lipid productivity of 80.23 mgL-1d-1 was achieved with the developed strategy under limited N (750 mg L-1) condition which was 2.18 fold higher than BG11 medium and 1.89 fold higher than N limited condition alone. Similarly, for C. sorokiniana, highest lipid productivity of 77.03 mgL-1d-1 was achieved with the developed strategy under limited N (500 mgL-1) and P (10 mgL-1) which was 2.67 fold higher than BG11 medium and 2.35 fold higher than N and P limited condition alone. For both the microalgal strains, Fe was the most significant trace metal affecting their lipid productivity. These above observations were further confirmed through photosynthetic performance analysis and gene expression studies. At mid log phase, 6.38 and 5.15 fold increases in the expression levels of rbcL gene were observed under combined stress (OCMS+OE) as compared to the control (BG11) condition in A. obliquus and C. sorokiniana respectively. This also resulted in an increased expression level of accD gene involved in lipid biosynthesis to 10.25 fold and 9.79 fold in A. obliquus and C. sorokiniana respectively at late log phase. The results from expression studies of rbcL and accD genes were in compliance with biomass yields, photosynthetic performance, protein yield and lipid productivities for both the strains under different cultivating conditions. The universal applicability of the above strategy was confirmed by applying it to five other microalgae strains isolated in this study which resulted in considerable increase in their overall lipid productivity under optimized conditions. Attempts were made to scale up the lab scale study to open circular pond (3000L) cultivation for A. obliquus. Results showed a 2.08 fold increase in lipid productivity under optimized conditions compared to the control, which emphasizes the scalability of the developed strategy even under uncontrolled conditions. In conclusion, the developed combined metal and EDTA stress strategy not only assisted in alleviating the biomass productivity but also enhanced the lipid accumulation which resulted in overall increased lipid productivity under N and P limited condition. Furthermore, the improved carbohydrate and protein productivities observed with the developed lipid enhancement strategy make it suitable for biorefinery approach with multiple products. An improvement in lipid profile and high biodiesel conversion were also observed with this universally applicable and scalable lipid enhancement strategy confirming their potential applicability during large scale cultivation for biodiesel production.

scholarly journals Investigation of Growth, Lipid Productivity, and Fatty Acid Profiles in Marine Bloom-Forming Dinoflagellates as Potential Feedstock for Biodiesel

2020 ◽  
Vol 8 (6) ◽  
pp. 381
Author(s):  
Steven Jingliang Xu ◽  
Kam-Chau Wu ◽  
Sophie Cheuk-Yan Chan ◽  
Yiu-Hung Yau ◽  
Kin-Ka Chan ◽  
...  
Keyword(s):  
Growth Rate ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Green Alga ◽  
Algal Bloom ◽  
Harmful Algal Bloom ◽  
Total Lipid Content ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Significant Difference

Microalgae-based biodiesel is increasingly recognized as an alternative to crop-based biodiesel. In this study, 10 local strains of dinoflagellates collected from Hong Kong waters, including a monoculture and field sample of Scrippsiella sp. isolated from an algal bloom, were evaluated against the performance of green alga Tetraselmis suecica. The specific growth rate, biomass production, lipid productivity, and fatty acid profile were investigated. The total lipid content of isolated strains ranged from 16.2% to 32.2% of the total dry biomass, whereas palmitic acid (C16:0) and docosahexaenoic acid (DHA, C22:6n3) were dominant in the fatty acid profile. Scrippsiella sp. has a high lipid productivity (47.3 mg/L/day) and fatty acid methyl esters (FAME) content (55.2–73 mg/g dry weight (dw)), which were comparable to that in green alga T. suecica. Further, monoculture and field sampled blooming Scrippsiella sp. showed no significant difference in most parameters, suggesting the possibility of harvesting a natural algal bloom population as a mitigation strategy to harmful algal bloom and to use as biodiesel feedstock. Overall, dinoflagellate species showed a slower growth rate (0.04–0.57 day−1) than most compared species (0.07–1.34 day−1), likely due to a large genome size and low chlorophyll to carbon ratio. Notably, most investigated dinoflagellates were not ideal for mass biodiesel production due to the low growth rate and lipid productivity. However, a high level of polyunsaturated fatty acids (PUFA) in dinoflagellates are prospective for further studies in other biotechnological applications. Though effectively harvesting algal blooming biomass can be complex, it can be further explored as a strategy for algal bloom mitigation and potentially creating values at the advantage of natural bloom when applying harvested biomass for biodiesel and bioactive compounds extraction.

Light intensity control as a strategy to improve lipid productivity in Chlorella sp. HS2 for biodiesel production

2019 ◽  
Vol 126 ◽  
pp. 211-219 ◽  
Author(s):  
Dong Woo Kim ◽  
Won-Sub Shin ◽  
Min-Gyu Sung ◽  
Bongsoo Lee ◽  
Yong Keun Chang
Keyword(s):  
Light Intensity ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Chlorella Sp ◽  
Intensity Control
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