Enzymatic Hydrolysis of Corn Stover after Pretreatment with Dilute Sulfuric Acid

2007 ◽  
Vol 30 (7) ◽  
pp. 938-944 ◽  
Author(s):  
X. B. Lu ◽  
Y. M. Zhang ◽  
J. Yang ◽  
Y. Liang
Keyword(s):  
Sulfuric Acid ◽  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Dilute Sulfuric Acid ◽  
Hydrolysis Of

Study on Dilute Sulfuric Acid Hydrolysis of Corn Stover

2011 ◽  
Vol 236-238 ◽  
pp. 313-316
Author(s):  
Jun Ping Zhu ◽  
Jian Lu Ma ◽  
Xiao Chen Liu ◽  
Biao Zhang ◽  
Wei Jie Xu ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Sulfuric Acid ◽  
Corn Stover ◽  
Optimal Conditions ◽  
Liquid Ratio ◽  
Factors Influencing ◽  
Dilute Sulfuric Acid ◽  
Hydrolysis Temperature ◽  
Solid Liquid ◽  
Hydrolysis Of

The object of this research is to investigate the factors influencing the hydrolysis of corn stover by dilute sulfuric acid and the types of sugars existed in hydrolysate. Experiment results showed that the optimal conditions were hydrolysis temperature 120°C, stover size 20-40 mesh, soak time 36h, reaction time 100 min, H2SO4concentration 1.5% and solid-liquid ratio 1:8. Sugars gained in the hydrolysate were xylose 3.0%, glucose 0.71%, mannose 0.12% and galactose 0.32% determined by capillary electrophoresis.

Dilute Sulfuric Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover into Fermentable Sugars

2012 ◽  
Vol 535-537 ◽  
pp. 2462-2468 ◽  
Author(s):  
Shang Xing Chen ◽  
Qiang Yong ◽  
Yong Xu ◽  
Shi Yuan Yu
Keyword(s):  
Sulfuric Acid ◽  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Acid Pretreatment ◽  
Pretreatment Condition ◽  
Structure And Property ◽  
Dilute Sulfuric Acid ◽  
Feature Structure ◽  
Dilute Sulfuric Acid Pretreatment ◽  
Sulfuric Acid Pretreatment

The pretreatment of corn stover with dilute sulfuric acid has been investigated by varying the acid concentration (0.5%-1.25%(w/w)) and the temperature (130-160°C). The pretreatment is aimed at improving enzymatic hydrolysis and increasing the fermentability of the biomass. Given the overall sugar yield, the most favourable pretreatment condition was performed with 0.75% sulfuric acid at 150°C for 30min and then with an enzyme loading of cellulase 15 FPU per gram of cellulose, and it resulted in a total of 49.74g glucose and xylose from 100g dry corn stover. The fiber physical feature, structure and property of pretreated residues were studied with Scanning Electron Microscope (SEM) and Fourier transform infrared spectroscopy. The SEM pictures indicated that the biomass structure was deformed and its fibers were exposed by the pretreatment. FTIR study showed that lignin and hemicellulose were partially removed during the diluted sulfuric acid pretreatment.

scholarly journals Effect of dilute sulfuric acid pretreatment on the physicochemical properties and enzymatic hydrolysis of coffee cut-stems

Energy ◽  
2020 ◽  
Vol 195 ◽  
pp. 116986 ◽  
Author(s):  
Juan Camilo Solarte-Toro ◽  
Yessica Chacón-Pérez ◽  
Sara Piedrahita-Rodríguez ◽  
Jhonny Alejandro Poveda Giraldo ◽  
José António Teixeira ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Enzymatic Hydrolysis ◽  
Physicochemical Properties ◽  
Acid Pretreatment ◽  
Dilute Sulfuric Acid ◽  
Dilute Sulfuric Acid Pretreatment ◽  
Sulfuric Acid Pretreatment ◽  
Hydrolysis Of

scholarly journals THF co-solvent pretreatment prevents lignin redeposition from interfering with enzymes yielding prolonged cellulase activity

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Abhishek S. Patri ◽  
Ramya Mohan ◽  
Yunqiao Pu ◽  
Chang G. Yoo ◽  
Arthur J. Ragauskas ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Sulfuric Acid ◽  
Enzymatic Hydrolysis ◽  
Cellulase Activity ◽  
Biofuel Production ◽  
Scanning Electron Micrographs ◽  
Enzyme Deactivation ◽  
Dilute Sulfuric Acid ◽  
Nmr Characterization ◽  
Hydrolysis Of

Abstract Background Conventional aqueous dilute sulfuric acid (DSA) pretreatment of lignocellulosic biomass facilitates hemicellulose solubilization and can improve subsequent enzymatic digestibility of cellulose to fermentable glucose. However, much of the lignin after DSA pretreatment either remains intact within the cell wall or readily redeposits back onto the biomass surface. This redeposited lignin has been shown to reduce enzyme activity and contribute to rapid enzyme deactivation, thus, necessitating significantly higher enzyme loadings than deemed economical for biofuel production from biomass. Results In this study, we demonstrate how detrimental lignin redeposition on biomass surface after pretreatment can be prevented by employing Co-solvent Enhanced Lignocellulosic Fractionation (CELF) pretreatment that uses THF–water co-solvents with dilute sulfuric acid to solubilize lignin and overcome limitations of DSA pretreatment. We first find that enzymatic hydrolysis of CELF-pretreated switchgrass can sustain a high enzyme activity over incubation periods as long as 5 weeks with enzyme doses as low as 2 mg protein/g glucan to achieve 90% yield to glucose. A modified Ninhydrin-based protein assay revealed that the free-enzyme concentration in the hydrolysate liquor, related to enzyme activity, remained unchanged over long hydrolysis times. DSA-pretreated switchgrass, by contrast, had a 40% drop in free enzymes in solution during incubation, providing evidence of enzyme deactivation. Furthermore, measurements of enzyme adsorption per gram of lignin suggested that CELF prevented lignin redeposition onto the biomass surface, and the little lignin left in the solids was mostly integral to the original lignin–carbohydrate complex (LCC). Scanning electron micrographs and NMR characterization of lignin supported this observation. Conclusions Enzymatic hydrolysis of solids from CELF pretreatment of switchgrass at low enzyme loadings was sustained for considerably longer times and reached higher conversions than for DSA solids. Analysis of solids following pretreatment and enzymatic hydrolysis showed that prolonged cellulase activity could be attributed to the limited lignin redeposition on the biomass surface making more enzymes available for hydrolysis of more accessible glucan.

scholarly journals Ethylene glycol based acid pretreatment of corn stover for cellulose enzymatic hydrolysis

RSC Advances ◽  
2021 ◽  
Vol 11 (23) ◽  
pp. 14140-14147
Author(s):  
Fengyang Xue ◽  
Wenzhi Li ◽  
Shengxin An ◽  
Cunshuo Li ◽  
Xu Li ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Enzymatic Hydrolysis ◽  
Ethylene Glycol ◽  
Corn Stover ◽  
Acid Pretreatment ◽  
Highly Efficient ◽  
Dilute Sulfuric Acid

A highly efficient pretreatment strategy using ethylene glycol with dilute sulfuric acid was developed for the fractionation of lignocellulose.

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