scholarly journals Enzyme Immobilization on Synthesized Nanoporous Silica Particles and their Application in a Bi‐enzymatic Reaction

ChemCatChem ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2245-2252 ◽  
Author(s):  
Claudia Engelmann ◽  
Narendhiran Ekambaram ◽  
Jens Johannsen ◽  
Oliver Fellechner ◽  
Thomas Waluga ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Enzymatic Reaction ◽  
Silica Particles ◽  
Nanoporous Silica

Ultrahigh Surface Area Nanoporous Silica Particles via an Aero-Sol−Gel Process

Langmuir ◽  
2004 ◽  
Vol 20 (7) ◽  
pp. 2523-2526 ◽  
Author(s):  
S. H. Kim ◽  
B. Y. H. Liu ◽  
M. R. Zachariah
Keyword(s):  
Surface Area ◽  
Sol Gel ◽  
Silica Particles ◽  
Nanoporous Silica ◽  
Sol Gel Process

scholarly journals Biocatalytic Reduction of Formaldehyde to Methanol: Effect of pH on Enzyme Immobilization and Reactive Membrane Performance

2021 ◽  
Vol 16 (3) ◽  
pp. 472-480
Author(s):  
Norhayati Abdul Rahman ◽  
Fauziah Marpani ◽  
Nur Hidayati Othman ◽  
Nur Hashimah Alias ◽  
Junaidah Jai ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Enzymatic Reaction ◽  
Feed Solution ◽  
Permeate Flux ◽  
High Concentration ◽  
Enzyme Loading ◽  
Reaction Conversion ◽  
Observed Rejection ◽  
Pes Membrane ◽  
Reactive Membrane

Thermodynamic stabled CO2 molecules can be biocatalytically reduced to methanol via three cascade dehydrogenases (formate, formaldehyde and alcohol) with the aid of cofactor as the electron donor. In this study, Alcohol dehydrogenase (EC 1.1.1.1), the third step of the cascade enzymatic reaction which catalyzed formaldehyde (CHOH) to methanol (CH3OH) will be immobilized in an ultrafiltration membrane. The enzyme will be immobilized in the support layer of a poly(ether)sulfone (PES) membrane via a technique called fouling induced enzyme immobilization. The objective of this study is to evaluate the effect of varying pH (acid (pH 5), neutral (pH 7) and alkaline (pH 9)) of the feed solution during immobilization process of ADH in the membrane in terms of permeate flux, observed rejection, enzyme loading and fouling mechanism. The experiment was conducted in a pressure driven, dead-end stirred filtration cell. Reaction conversion and biocatalytic productivity will be also evaluated. The results showed that permeate flux for acid solution were the lowest during immobilization. High concentration polarization and fouling resistance cause lower observed rejection for pH 7 and 9. Enzyme loading for pH 5 give 73.8% loading rate which is the highest compared to 62.4% at pH 7 and 70.1% at pH 9. Meanwhile, the conversion rate during the reaction shows that reaction on fouled membrane showed more than 90% conversion for pH 5 and 7. The fouling model predicted that irreversible fouling occurs during enzyme immobilization at pH 7 with standard blocking mechanism while reversible fouling occurs at pH 5 and 9 with intermediate and complete blocking, respectively. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 

Relating Mobility of dsRNA in Nanoporous Silica Particles to Loading and Release Behavior

2021 ◽  
Author(s):  
Shanshan Zhou ◽  
Emily A. Nadeau ◽  
M. Arif Khan ◽  
Bruce A. Webb ◽  
Stephen E. Rankin ◽  
...  
Keyword(s):  
Silica Particles ◽  
Nanoporous Silica ◽  
Release Behavior

Preparation of submicrometer monodispersed magnetic silica particles using a novel water in oil microemulsion: properties and application for enzyme immobilization

2013 ◽  
Vol 35 (10) ◽  
pp. 1571-1577 ◽  
Author(s):  
Maria Victoria Tuttolomondo ◽  
Maria Emilia Villanueva ◽  
Gisela Solange Alvarez ◽  
Martín Federico Desimone ◽  
Luis Eduardo Díaz
Keyword(s):  
Enzyme Immobilization ◽  
Silica Particles ◽  
Magnetic Silica

Clofazimine encapsulation in nanoporous silica particles for the oral treatment of antibiotic-resistant Mycobacterium tuberculosis infections

Nanomedicine ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. 831-844 ◽  
Author(s):  
Sabrina Valetti ◽  
Xin Xia ◽  
Joana Costa-Gouveia ◽  
Priscille Brodin ◽  
Marie-Françoise Bernet-Camard ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Oral Treatment ◽  
Silica Particles ◽  
Nanoporous Silica ◽  
Antibiotic Resistant

Selective binding and enrichment for low-molecular weight biomarker molecules in human plasma after exposure to nanoporous silica particles

PROTEOMICS ◽  
2006 ◽  
Vol 6 (11) ◽  
pp. 3243-3250 ◽  
Author(s):  
Rosa Terracciano ◽  
Marco Gaspari ◽  
Flaviano Testa ◽  
Luigi Pasqua ◽  
Pierosandro Tagliaferri ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Plasma ◽  
Silica Particles ◽  
Nanoporous Silica ◽  
Low Molecular Weight ◽  
Selective Binding ◽  
Biomarker Molecules

Deposition of a titania layer on spherical porous silica particles and their nanostructure-induced vapor sensing properties

Nanoscale ◽  
2017 ◽  
Vol 9 (43) ◽  
pp. 16791-16799 ◽  
Author(s):  
K. Shiba ◽  
T. Takei ◽  
G. Yoshikawa ◽  
M. Ogawa
Keyword(s):  
Porous Silica ◽  
Silica Particles ◽  
Spherical Particles ◽  
Nanoporous Silica ◽  
Sensing Properties ◽  
Vapor Sensing ◽  
Porous Titania ◽  
Titania Layer

A porous titania layer with different nanostructures was formed on monodispersed nanoporous silica spherical particles to demonstrate unique sensitive/selective vapor sensing properties.

Comparison of the performance of pyridine-functionalized nanoporous silica particles for the extraction of gold(III) from natural samples

2012 ◽  
Vol 178 (3-4) ◽  
pp. 367-372 ◽  
Author(s):  
Elahe Moazzen ◽  
Nasser Daei ◽  
Seyyedeh Mahdieh Hosseini ◽  
Homeira Ebrahimzadeh ◽  
Azam Monfared ◽  
...  
Keyword(s):  
Silica Particles ◽  
Nanoporous Silica

Increased fracture toughness in nanoporous silica–polyimide matrix composites

2001 ◽  
Vol 16 (7) ◽  
pp. 1975-1981 ◽  
Author(s):  
Shiling Ruan ◽  
John J. Lannutti ◽  
Stan Prybyla ◽  
Robert R. Seghi
Keyword(s):  
Fracture Toughness ◽  
Sol Gel ◽  
Porous Silica ◽  
Silica Particles ◽  
Nanoporous Silica ◽  
Matrix Composites ◽  
Silica Powder ◽  
Crack Pinning ◽  
Cure Temperature ◽  
Polyimide Matrix

Silica–polyimide nanocomposites were prepared by hot-pressing mixtures of polyimide and highly porous silica powder. The silica powder was produced using a sol-gel process that generates pores as small as 15 Å. The effects of loading, cure, and post-cure temperature on fracture toughness were investigated. The addition of silica particles improved the fracture toughness from 0.5 to a maximum of 1.9 MPa m0.5. However, fracture toughness dropped at silica weight percentages ≥30%. The cure and post-curing temperatures have a strong influence on toughness; post-curing exposure ≥400 °C reduced toughness. Transmission electron microscopy examination of the fracture surfaces indicated that the toughness improvements may occur at the nanometer scale due to crack pinning and branching induced by the nanoporous silica particles.

EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME

2018 ◽  
Vol 80 (3) ◽  
Author(s):  
Suhaily Suhaimi ◽  
Rohaida Che Man ◽  
Natassha Jamil ◽  
Zatul Iffah Mohd Arshad ◽  
Shalyda Md Shaarani ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Hollow Fiber ◽  
Immobilized Enzyme ◽  
Hollow Fiber Membrane ◽  
Potato Starch ◽  
Enzymatic Reaction ◽  
Cyclodextrin Glucanotransferase ◽  
Fiber Membrane ◽  
Reaction Conditions ◽  
Agricultural Industries

The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.

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