Whole-Cell Biocatalysis in Deep-Eutectic-Solvents/Aqueous Mixtures

ChemCatChem ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 1535-1537 ◽  
Author(s):  
Zaira Maugeri ◽  
Pablo Domínguez de María
Keyword(s):  
Deep Eutectic Solvents ◽  
Aqueous Mixtures ◽  
Whole Cell ◽  
Whole Cell Biocatalysis

Improving Whole-Cell Biocatalysis by Addition of Deep Eutectic Solvents and Natural Deep Eutectic Solvents

2017 ◽  
Vol 5 (7) ◽  
pp. 5713-5722 ◽  
Author(s):  
Tao-Xiang Yang ◽  
Li-Qing Zhao ◽  
Juan Wang ◽  
Guo-Li Song ◽  
Hai-Min Liu ◽  
...  
Keyword(s):  
Deep Eutectic Solvents ◽  
Whole Cell ◽  
Whole Cell Biocatalysis ◽  
Natural Deep Eutectic Solvents

scholarly journals In Vivo Assembly of Artificial Metalloenzymes and Application in Whole‐Cell Biocatalysis**

2021 ◽  
Author(s):  
Shreyans Chordia ◽  
Siddarth Narasimhan ◽  
Alessandra Lucini Paioni ◽  
Marc Baldus ◽  
Gerard Roelfes
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalysis ◽  
Artificial Metalloenzymes

Enantiocomplementary decarboxylative hydroxylation combining photocatalysis and whole-cell biocatalysis in a one-pot cascade process

2019 ◽  
Vol 21 (8) ◽  
pp. 1907-1911 ◽  
Author(s):  
Jian Xu ◽  
Mamatjan Arkin ◽  
Yongzhen Peng ◽  
Weihua Xu ◽  
Huilei Yu ◽  
...  
Keyword(s):  
Cascade Process ◽  
One Pot ◽  
Whole Cell ◽  
Whole Cell Biocatalysis

The first demonstration of photochemo-enzymatic whole-cell one-pot enantiocomplementary decarboxylative hydroxylation.

Whole‐cell biocatalysis: Advancements toward the biosynthesis of fuels

2021 ◽  
Author(s):  
Tanushree Baldeo Madavi ◽  
Sushma Chauhan ◽  
Anushri Keshri ◽  
Hemasundar Alavilli ◽  
Kwon‐Young Choi ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalysis

scholarly journals Whole-Cell Biocatalysis for 1-Naphthol Production in Liquid-Liquid Biphasic Systems

2009 ◽  
Vol 75 (20) ◽  
pp. 6545-6552 ◽  
Author(s):  
S. V. B. Janardhan Garikipati ◽  
Angela M. McIver ◽  
Tonya L. Peeples
Keyword(s):  
Organic Phase ◽  
Calcium Alginate ◽  
Immobilized Cells ◽  
Aqueous Media ◽  
Dry Weight ◽  
Dioctyl Phthalate ◽  
Whole Cell ◽  
Whole Cell Biocatalysis ◽  
Naphthalene Concentration ◽  
Biphasic Systems

ABSTRACT Whole-cell biocatalysis to oxidize naphthalene to 1-naphthol in liquid-liquid biphasic systems was performed. Escherichia coli expressing TOM-Green, a variant of toluene ortho-monooxygenase (TOM), was used for this oxidation. Three different solvents, dodecane, dioctyl phthalate, and lauryl acetate, were screened for biotransformations in biphasic media. Of the solvents tested, lauryl acetate gave the best results, producing 0.72 ± 0.03 g/liter 1-naphthol with a productivity of 0.46 ± 0.02 g/g (dry weight) cells after 48 h. The effects of the organic phase ratio and the naphthalene concentration in the organic phase were investigated. The highest 1-naphthol concentration (1.43 g/liter) and the highest 1-naphthol productivity (0.55 g/g [dry weight] cells) were achieved by optimization of the organic phase. The ability to recycle both free cells and cells immobilized in calcium alginate was tested. Both free and immobilized cells lost more than ∼60% of their activity after the first run, which could be attributed to product toxicity. On a constant-volume basis, an eightfold improvement in 1-naphthol production was achieved using biphasic media compared to biotransformation in aqueous media.

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