Comparative evaluation of amino acid composition and protein profile of gliadin from different extraction protocols

2021 ◽  
Author(s):  
Monika Rani ◽  
Raashid Ahmad Siddiqi ◽  
Dalbir Singh Sogi ◽  
Balmeet Singh Gill
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Comparative Evaluation ◽  
Protein Profile

scholarly journals Studies on the structure of sphingomyelinase. Amino acid composition and heterogeneity on isoelectric focusing

1983 ◽  
Vol 209 (2) ◽  
pp. 291-297 ◽  
Author(s):  
C S Jones ◽  
P Shankaran ◽  
D J Davidson ◽  
A Poulos ◽  
J W Callahan
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Focusing ◽  
Protein Profile ◽  
Minor Component ◽  
Intact Protein ◽  
Amino Acid Residues ◽  
Apparent Homogeneity ◽  
A Minor

Sphingomyelinase, purified to apparent homogeneity from human placenta, is an acidic protein, as judged from its amino acid composition and by isoelectric focusing of the carboxymethylated protein. The amino acid composition is characterized by an approximately equal content of hydrophobic and polar amino acid residues. The reduced-alkylated polypeptides were separated into two groups. Most of the polypeptides were heterogeneous with pI values of 4.4-5.0, but an additional more minor component was observed at pI 5.4. Liquid isoelectric focusing resolved the purified enzyme into a single major component (pI 4.7-4.8), a minor component (pI 5.0-5.4) and a plateau region of activity (pI 6-7). On thin-layer isoelectric focusing, the protein profile obtained from each of these regions was the same. In addition, the substrate specificity, Km values and effect of inhibitory substances were identical. We conclude that sphingomyelinase is an acidic, microheterogeneous protein that likely exists as a holopolymer of a single major polypeptide chain. the heterogeneity of the intact protein on isoelectric focusing appears to reflect this microheterogeneity, which is influenced by a tendency to associate with itself and with detergents such as Triton X-100.

Effect of maternal diet on the amino acid composition of human uterine fluid

2014 ◽  
Author(s):  
Alexandra Jayne Kermack ◽  
Ying Cheong ◽  
Nick Brook ◽  
Nick Macklon ◽  
Franchesca D Houghton
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Maternal Diet ◽  
Uterine Fluid
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