Investigation of the effects of fermented wheat bran extract containing beta‐glucanase on beta‐glucan of cereals used in animal feed

2021 ◽  
Author(s):  
Masoumeh Heidary Vinche ◽  
Morteza Khanahmadi ◽  
Seyed Ahmad Ataei ◽  
Firoozeh Danafar
Keyword(s):  
Wheat Bran ◽  
Animal Feed ◽  
Beta Glucan ◽  
Beta Glucanase ◽  
Wheat Bran Extract

scholarly journals WHEAT BRAN EXTRACT MIMICS STATIN LIKE ACTION IN HYPERLIPIDEMIC RABBITS: AN EXPERIMENTAL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 402 ◽  
Author(s):  
Rashi Chadha ◽  
Ashok Purohit
Keyword(s):  
Lipid Profile ◽  
Wheat Bran ◽  
Serum Lipid ◽  
Ethanolic Extract ◽  
Serum Lipid Profile ◽  
Histopathological Study ◽  
Standard Drug ◽  
Reducing Ability ◽  
Atheromatous Plaques ◽  
Wheat Bran Extract

 Objective: The study was aimed to evaluate the hypolipidemic and antioxidant effect of Triticum aestivum (wheat) bran extraction cholesterol-fed rabbits.Methods: The induced hyperlipidemic rabbits were treated with 70% ethanolic extract of wheat bran at a dose of 500 mg/kg body weight/day. The experimental protocol was designed for control, hyperlipidemic, wheat bran extract, and statin-treated groups for 60 days. The estimations for serum lipid profile, lipid peroxidation (LPO), and ferric reducing ability of plasma (FRAP) were done, and histopathological study of thoracic aorta was carried out.Results: Oral administration of wheat bran extract caused significant reductions in the serum lipid profile by 94.30% (total cholesterol), 96.73% (low-density lipoprotein cholesterol), and 58.28% (triglycerides). It significantly decreased the LPO and enhanced the antioxidant capacity (FRAP) activity as compared to the hyperlipidemic rabbits. Histopathology revealed that wheat bran extract treatment prevented the accumulation of cholesterol and regressed atheromatous plaques significantly in the aorta as compared to the hyperlipidemic group, and the results were comparable to that of the standard drug statin.Conclusion: The study exhibited that wheat bran extract controls atherosclerosis just like statin and can be used as a regular dietary supplement to manage cholesterol levels and prevent cardiovascular problems.

scholarly journals Potential methodologies and strategies for the rapid assessment of feed-grain quality

1999 ◽  
Vol 50 (5) ◽  
pp. 789 ◽  
Author(s):  
C. W. Wrigley
Keyword(s):  
Grain Quality ◽  
Near Infrared ◽  
Animal Feed ◽  
Rapid Assessment ◽  
Cost Effective ◽  
Beta Glucan ◽  
Site Analysis ◽  
Direct Indication ◽  
Systematic Strategy

The efficient use of grains for animal feed requires the use of analytical methods that can provide rapid indications of the suitability of the grain for animal nutrition. Ideally, these methods need to be applied at the site of grain receival within the tight time and cost confines of grain delivery. In addition, methods are needed in plant breeding to efficiently screen for target aspects of feed-grain quality to facilitate the development of genotypes with improved nutritional quality. This review describes a range of techniques that can fulfil these analytical requirements. These include visual examination of grain samples for species identification and for recognition of defects and contaminants. This long-standing approach is rapid, but it is subjective and dependent on the expertise of the operator. The newer technology of image analysis offers the prospect of providing similar information automatically and quantitatively, without the risk of operator bias. Near-infrared (NIR) analysis is already in general use for grain analysis at many receival depots in wheat-growing countries, mainly for the determination of moisture and protein content. There are promising indications that NIR can be extended to the determination of many other aspects of grain composition, both the positive aspects that contribute to feed quality, as well as components such as beta-glucan content that have negative contributions for non-ruminants. Furthermore, NIR is being developed to provide a direct indication of metabolisable energy for a range of grain types. Whereas NIR is primarily suited to the determination of quantitatively major components of the grain, without the requirement of significant sample preparation, immunological analyses are appropriate for the determination of specific and minor components, such as mycotoxins, lectins, alkaloids, and pesticide residues. These and other methods, suited for on-site analysis, need to be combined with effective sampling to ensure that the results of testing are representative of the whole of the grain consignment, and also integrated into a systematic strategy to ensure cost-effective testing.

scholarly journals Optimizing Laccase Production in Auricularia Cornea by Submerged Fermentation With Wheat Bran Extract: Applications in Decolorization of Malachite Green Dye

2021 ◽  
Author(s):  
Li Meng ◽  
Xiaoran Bai ◽  
Qingji Wang ◽  
Xiaobo Li ◽  
Shaoyan Zhang ◽  
...  
Keyword(s):  
Malachite Green ◽  
Wheat Bran ◽  
Submerged Fermentation ◽  
High Performance ◽  
Laccase Activity ◽  
Degradation Products ◽  
Laccase Production ◽  
Malachite Green Dye ◽  
Chromosome Bridge ◽  
Wheat Bran Extract

Abstract Wheat bran extract may enhance laccase activity of Auricularia cornea in submerged fermentation. The laccase activity of A. cornea was maximal (768.78 U/mL) at 26.9 ℃, pH 5, a time point of 6 d, 22 h, and an inducer concentration of 10%. Laccase from A. cornea AC5 decolorized 83.27% of 75 mg/L malachite green within 12 h. High performance liquid chromatography (HPLC) analysis of the extracted metabolites suggested that the decolorization occurred through biodegradation. Malachite green induced cytotoxic damage including formation of a micronucleus and chromosome bridge at anaphase. Degradation products of malachite green proved to be less toxic and had negligible effects on chromosomal aberrations.

Nano Polyphenols and Nano Tocopherols from Wheat Bran Extract

2019 ◽  
Vol 10 (1) ◽  
pp. 16-22
Author(s):  
Abdalla S. M. Ammar ◽  
Sobhy M. Mohsen ◽  
Hoda G. El-Amry ◽  
Reham S. Abd El-Salam
Keyword(s):  
Wheat Bran ◽  
Wheat Bran Extract

Cloning and characterization of KNR4, a yeast gene involved in (1,3)-beta-glucan synthesis

1994 ◽  
Vol 14 (2) ◽  
pp. 1017-1025
Author(s):  
Z Hong ◽  
P Mann ◽  
N H Brown ◽  
L E Tran ◽  
K J Shaw ◽  
...  
Keyword(s):  
Cell Wall ◽  
Killer Toxin ◽  
Structural Defects ◽  
Beta Glucan ◽  
Calculated Molecular Mass ◽  
Beta Glucanase ◽  
Reading Frame ◽  
Glucan Synthesis ◽  
The Right

k9 killer toxin from Hansenula mrakii was used to select a number of resistant mutants from Saccharomyces cerevisiae. Preliminary biochemical and genetic studies showed that some of them acquired structural defects in the cell wall. One of these mutants, the knr4-1 mutant, displays a number of cell wall defects, including osmotic sensitivity; sensitivity to cercosporamide, a known antifungal agent; and resistance to Zymolyase, a (1,3)-beta-glucanase. We report here the isolation and analysis of the KNR4 gene. DNA sequence analysis revealed an uninterrupted open reading frame which contains five potential start codons. The longest coding template encodes a protein of 505 amino acids with a calculated molecular mass of 57,044 Da. A data base search revealed 100% identity with a nuclear protein, SMI1p. Disruption of the KNR4 locus does not result in cell death; however, it leads to reduced levels of both (1,3)-beta-glucan synthase activity and (1,3)-beta-glucan content in the cell wall. The gene was mapped to the right arm of chromosome VII.

scholarly journals A Simulation Model for the Control of beta-Glucanase Activity and beta-Glucan Degradation During Germination in Malting

2004 ◽  
Vol 110 (4) ◽  
pp. 309-319 ◽  
Author(s):  
Pirkko Kuusela ◽  
Jari J. Hämäläinen ◽  
Pekka Reinikainen ◽  
Juhani Olkku
Keyword(s):  
Simulation Model ◽  
Beta Glucan ◽  
Glucanase Activity ◽  
Beta Glucanase
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