An alternative treatment strategy for large vessel coronary perforations

2018 ◽  
Vol 93 (4) ◽  
pp. 635-638 ◽  
Author(s):  
Iosif Xenogiannis ◽  
Peter Tajti ◽  
M. Nicholas Burke ◽  
Emmanouil S. Brilakis
Keyword(s):  
Alternative Treatment ◽  
Treatment Strategy ◽  
Large Vessel ◽  
Alternative Treatment Strategy

Prasugrel as alternative treatment strategy in a case with a hypersensitivity reaction to clopidogrel

Platelets ◽  
2010 ◽  
Vol 22 (1) ◽  
pp. 75-76 ◽  
Author(s):  
Jochem W. van Werkum ◽  
Thijs L. Braber ◽  
Peter W. H. M. Verheggen ◽  
Simone M. Van Der Have-Roeffel
Keyword(s):  
Hypersensitivity Reaction ◽  
Alternative Treatment ◽  
Treatment Strategy ◽  
Alternative Treatment Strategy

An Alternative Treatment Strategy for Pump Thrombus in the DeBakey VAD Child: Use of Clopidogrel as a Thrombolytic Agent

2006 ◽  
Vol 25 (7) ◽  
pp. 857-861 ◽  
Author(s):  
Matthew A. Studer ◽  
Curtis E. Kennedy ◽  
William J. Dreyer ◽  
Jack F. Price ◽  
Brady S. Moffett ◽  
...  
Keyword(s):  
Alternative Treatment ◽  
Treatment Strategy ◽  
Thrombolytic Agent ◽  
Alternative Treatment Strategy

scholarly journals Fahotyp and Sensitivity to Medications Phage Film-Forming Strains Staphylococcus aureus, Isolated from Respiratory Tract

2016 ◽  
Vol 51 ◽  
pp. 1-5
Author(s):  
Olga S. Voronkova ◽  
Albert I. Vinnikov
Keyword(s):  
Staphylococcus Aureus ◽  
Respiratory Tract ◽  
Alternative Treatment ◽  
Treatment Strategy ◽  
Phage Type ◽  
Biological Properties ◽  
Diagnostic Service ◽  
Alternative Treatment Strategy ◽  
Film Forming ◽  
Nose And Throat

The study of biological properties of filmforming Staphylococcus aureus strains, including its sensitivity to bacteriophages, is one of the important tasks of laboratory diagnostic service that allows, if it necessary, choose an alternative treatment strategy and to establish the sources of strains. In the study of material from the nose and throat of 26 persons-volunteers the strains of S. aureus were selected. Among them 16 (61.5%) were able to form biofilms. Analysis of resistance to phages drugs of filmformation strains of S. aureus showed that 87.5% strains were sensitive the phages drugs "Piobacteriophage" and "Bacteriophage staphylococcal liquid". Phage type was identified for 9 (56.3%) strains. 64.3% of these strains were sensitive to the 1 phage from set, 21.4% – to 2 and 14.3% – 3 phages. It was determined that 77.8% strains were typed by phages from third group. For 1 strain detected simultaneous sensitivity to phages of 2 and 3 groups. With phage 81 (out of group phage) interacted 4 strains: 2 showed sensitivity only to him and another 2 were also susceptible to phage of third group.

Cross-genus Boot-up of Synthetic Bacteriophage in Staphylococcus aureus Using a New and Efficient DNA Transformation Method

2021 ◽  
Author(s):  
Nacyra Assad-Garcia ◽  
Roshan D’Souza ◽  
Rachel Buzzeo ◽  
Arti Tripathi ◽  
Lauren M. Oldfield ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Alternative Treatment ◽  
Treatment Strategy ◽  
Bacterial Resistance ◽  
Rapid Development ◽  
Transformation Method ◽  
Dna Transformation ◽  
Efficient Transformation ◽  
Alternative Treatment Strategy ◽  
Wide Range

Staphylococcus aureus is an opportunistic pathogen causing a wide range of infections and food poisoning in humans with antibiotic resistance, specifically to methicillin, compounding the problem. Bacteriophages (phages) provide an alternative treatment strategy, but only infect a limited number of circulating strains and may quickly become ineffective due to bacterial resistance. To overcome these obstacles, engineered phages have been proposed, but methods are needed for efficient transformation of large DNA molecules into S. aureus to boot-up (i.e., rescue) infectious phages. We present a new, efficient and reproducible DNA transformation method, NEST (Non-Electroporation Staphylococcus Transformation), for S. aureus to boot-up of purified phage genomic DNA (at least 150 kb in length tested) and whole yeast-assembled synthetic phage genomes. This method is a powerful new tool for transformation of DNA in S. aureus and will enable the rapid development of engineered therapeutic phages and phage cocktails against Gram-positive pathogens. Importance The continued emergence of antibiotic resistant bacterial pathogens has heightened the urgency for alternative antibacterial strategies. Phages provide an alternative treatment strategy, but are difficult to optimize. Synthetic biology approaches have been successfully used to construct and rescue genomes of model phages, but only in a limited number of highly transformable host species. In this study, we used a new, reproducible, and efficient transformation method to reconstitute a functional non-model Siphophage from a constructed synthetic genome. This method will facilitate not only the engineering of Staphylococcus and Enterococcus phages for therapeutic applications but also the engineering of Staphylococcus strains by enabling transformation of higher molecular weight DNA to introduce more complex modifications.

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